Effect of sodium butyrate on the production, heterogeneity and biological activity of human thrombopoietin by recombinant Chinese hamster ovary cells

Journal of Biotechnology

Volumn 112, Issue 3, 2004, Pages 323-335

  Sung, Yun Hee ^a   Song, Yeon Jung ^b   Lim, Seung Wook ^b   Chung, Joo Young ^b   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Daewoong Pharmaceutical Co Ltd   (South Korea)

Author keywords

Apoptosis; Biological activity; CHO cells; Human thrombopoietin; Salic acid; Sodium butyrate

Indexed keywords

BIOTECHNOLOGY; CELL CULTURE; CELLS; CONCENTRATION (PROCESS);

GLYCOPROTEINS; ISOFORMS;

PROTEINS;

BUTYRIC ACID; THROMBOPOIETIN;

ANIMAL CELL; ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; CELL CULTURE; CELL GROWTH; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; DRUG ACTIVITY; DRUG SYNTHESIS; FEMALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SIALYLATION; VOLUMETRY;

ANIMALS; APOPTOSIS; BUTYRIC ACIDS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION REGULATION; HUMANS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SODIUM; THROMBOPOIETIN;

CRICETINAE; CRICETULUS GRISEUS;

EID: 4143145162     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2004.05.003     Document Type: Article

References (37)

1. Ashwell G., Harford J. Carbohydrate-specific receptors of the liver. Annu. Rev. Biochem. 51:1982;531-554
2. Bartley T.D., Bogenberger J., Hunt P., Li Y.S., Lu H.S., Martin F., Chang M.S., Samal B., Nichol J.L., Swift S., Johnson M.J., Hsu R.Y., Parker V.P., Suggs S., Skrine J.D., Merewether L.A., Clogston C., Hsu E., Hokom M.M., Hornkohl A., Choi E., Pangelinan M., Sun Y., Mar V., McNinch J., Simonet L., Jacobsen F., Xie C., Shutter J., Chute H., Basu R., Selander L., Trollinger D., Sieu L., Padilla D., Trail G., Elliott G., Izumi R., Covey T., Crouse J., Garcia A., Xu W., Del Castillo J., Biron J., Cole S., Hu M.C.T., Pacifici R., Ponting I., Saris C., Wen D., Yung Y.P., Lin H., Bosselman R.A. Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor Mpl. Cell. 77:1994;1117-1124
3. Chang K.H., Kim K.S., Kim J.H. N-Acetylcysteine increases the biosynthesis of recombinant EPO in apoptotic Chinese hamster ovary cells. Free Radic. Res. 30:1999;85-91
4. Chotigeat W., Watanapokasin Y., Mahler S., Gray P.P. Role of environmental conditions on the expression levels, glycoform pattern and levels of sialyltransferase for hFSH produced by recombinant CHO cells. Cytotechnology. 15:1994;217-221
5. Chung B.S., Jeong Y.T., Chang K.H., Kim J.S., Kim J.H. Effect of sodium butyrate on glycosylation of recombinant erythropoietin. J. Microbiol. Biotechnol. 11:2001;1087-1092
6. Chung J.Y., Ahn H.K., Lim S.W., Sung Y.H., Koh Y.W., Park S.K., Lee G.M. Development of recombinant Chinese hamster ovary cell lines producing human thrombopoietin or its analog. J. Microbiol. Biotechnol. 13:2003;759-766
7. Cockett M.I., Bebbington C.R., Yarranton G.T. High-level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification. Biotechnology. 8:1990;662-667
8. Datti A., Dennis J.W. Regulation of UDP-GlcNAc:Gal(1-3GalNAc-R (1-6-N-acetylglucosaminyltransferase (GlcNAc to GalNAc) in Chinese hamster ovary cells. J. Biol. Chem. 268:1993;5409-5416
9. Gorman C.M., Howard B.H., Reeves R. Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate. Nucleic Acids Res. 11:1983;7631-7648
10. Gramer M.J., Goochee C.F. Glycosidase activities in Chinese hamster ovary cell lysate and cell culture supernatant. Biotechnol. Prog. 9:1993;366-373
11. Gramer M.J., Goochee C.F., Chock V.Y., Brousseau D.T., Sliwkowski M.B. Removal of sialic acid from a glycoprotein in CHO cell culture supernatant by action of an extracellular CHO cell sialidase. Biotechnology. 13:1995;692-698
12. Hall C.W., Robbins A.R., Krag S.S. Preliminary characterization of a Chinese hamster ovary cell glycosylation mutant isolated by screening for low intracellular lysosomal enzyme activity. Mol. Cell. Biochem. 72:1986;35-45
13. Hasilik A., Neufeld E.F. Biosynthesis of lysosomal enzymes in fibroblasts. J. Biol. Chem. 255:1980;4937-4945
14. Hendrick V., Winnepenninckx P., Abdelkafi C., Vandeputte O., Cherlet M., Marique T., Renemann G., Loa A., Kretzmer G., Werenne J. Increased productivity of recombinant tissular plasminogen activator (t-PA) by butyrate and shift of temperature: a cell cycle phases analysis. Cytotechnology. 36:2001;71-83
15. Hoffman R.C., Andersen H., Walker K., Krakover J.D., Patel S., Stamm M.R., Osborn S.G. Peptide, disulfide, and glycosylation mapping of recombinant human thrombopoietin from Ser1 to Arg246. Biochemistry. 35:1996;14849-14861
16. Hokom M.M., Lacey D., Kinstler O.B., Choi E., Kaufman S., Faust J., Rowan C., Dwyer E., Nichol J.L., Grasel T., Wilson J., Steinbrink R., Hecht R., Winters D., Boone T., Hunt P. Pegylated megakaryocyte growth and development factor abrogates the lethal thrombocytopenia associated with carboplatin and irradiation in mice. Blood. 86:1995;4486-4492
17. Hunt L., Batard P., Jordan M., Wurm F.M. Fluorescent proteins in animal cells for process development: optimization of sodium butyrate treatment as an example. Biotechnol. Bioeng. 77:2002;528-537
18. Kato T., Matsumoto A., Ogami K., Tahara T., Morita H., Miyazaki H. Native thrombopoietin: structure and function. Stem Cells. 16:1998;322-328
19. Kim N.S., Lee G.M. Overexpression of bcl-2 inhibits sodium butyrate-induced apoptosis in Chinese hamster ovary cells resulting in enhanced humanized antibody production. Biotechnol. Bioeng. 71:2001;184-193
20. Kim N.S., Lee G.M. Inhibition of sodium butyrate-induced apoptosis in recombinant Chinese hamster ovary cells by constitutively expressing antisense RNA of caspase-3. Biotechnol. Bioeng. 78:2002;217-228
21. Laubach V.E., Garvey E.P., Sherman P.A. High-level expression of human inducible nitric oxide synthase in Chinese hamster ovary cells and characterization of the purified enzyme. Biochem. Biophys. Res. Commun. 218:1996;802-807
22. Lee E.U., Roth J., Paulson J.C. Alteration of terminal glycosylation sequences on N-linked oligosaccharides of Chinese hamster ovary cells by expression of β-galactoside α2,6-sialyltransferase. J. Biol. Chem. 264:1989;13848-13855
23. Mercille S., Massie B. Apoptosis-resistant E1B-19K-expressing NS/0 myeloma cells exhibit increased viability and chimeric antibody productivity under perfusion culture conditions. Biotechnol. Bioeng. 63:1999;529-543
24. Mimura Y., Lund J., Church S., Dong S., Li J., Goodall M., Jefferis R. Butyrate increases production of human chimeric IgG in CHO-K1 cells whilst maintaining function and glycoform profile. J. Immunol. Methods. 247:2001;205-216
25. Moore A., Mercer J., Dutina G., Donahue C.J., Bauer K.D., Mather J.P., Etcheverry T., Ryll T. Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultures. Cytotechnology. 23:1997;47-54
26. Morimoto K., Tsuda E., Said A.A., Uchida E., Hatakeyama S., Ueda M., Hayakawa T. Biological and physicochemical characterization of recombinant human erythropoietins fractionated by Mono Q column chromatography and their modification with sialytransferase. Glycoconj. J. 13:1996;1013-1020
27. Mulders J.W.M., Derksen M., Swolfs A., Maris F. Prediction of the in vivo biological activity of human recombinant follicle stimulating hormone using quantitative isoelectric focusing. Biologicals. 25:1997;269-281
28. Munzert E., Muthing J., Buntemeyer H., Lehmann J. Sialidase activity in culture fluid of Chinese hamster ovary cells during batch culture and its effect on recombinant human antithrombin III integrity. Biotechnol. Prog. 12:1996;559-563
29. Oster T., Thioudellet C., Chevalot I., Masson C., Wellman M., Marc A., Siest G. Induction of recombinant human gamma-glutamyl transferase by sodium butyrate in transfected V79 and CHO Chinese hamster cells. Biochem. Biophys. Res. Commun. 193:1993;406-412
30. Palermo D.P., DeGraaf M.E., Marotti K.R., Rehberg E., Post L.E. Production of analytical quantities of recombinant proteins in Chinese hamster ovary cells using sodium butyrate to elevate gene expression. J. Biotechnol. 19:1991;35-48
31. Renard J.M., Spagnoli R., Mazier C., Salles M.F., Mandine E. Evidence that monoclonal antibody production kinetics is related to the integral of viable cells in batch systems. Biotechnol. Lett. 10:1988;91-96
32. Robbins A.R. Isolation of lysosomal α-mannosidase mutants of Chinese hamster ovary cells. Proc. Natl. Acad. Sci. U.S.A. 76:1979;1911-1915
33. Santell L., Ryll T., Etcheverry T., Santoris M., Dutina G., Wang A., Gunson J., Warner T.G. Aberrant metabolic sialylation of recombinant proteins expressed in Chinese hamster ovary cells in high productivity cultures. Biochem. Biophys. Res. Commun. 258:1999;132-137
34. Shah S., Lance P., Smith T.J., Berenson C.S., Cohen S.A., Horvath P.J., Lau J.T.Y., Baumann H. n-Butyrate reduces the expression of β-galactoside α2,6-sialyltransferase in Hep G2 cells. J. Biol. Chem. 267:1992;10652-10658
35. Sung Y.H., Lim S.W., Chung J.Y., Lee G.M. Yeast hydrolysate as a low-cost additive to serum-free medium for the production of human thrombopoietin in suspension cultures of Chinese hamster ovary cells. Appl. Microbiol. Biotechnol. 63:2004;527-536
36. Traving C., Schauer R. Structure, function and metabolism of sialic acids. Cell. Mol. Life Sci. 54:1998;1330-1349
37. Ulich T.R., del Castillo J., Yin S., Swift S., Padilla D., Senaldi G., Bennett L., Shutter J., Bogenberger J., Sun D., Samal B., Shimamoto G., Lee R., Steinbrink R., Boone T., Sheridan W.T., Hunt P. Megakaryocyte growth and development factor ameliorates carboplatin-induced thrombocytopenia in mice. Blood. 86:1995;971-976