Cyclic GMP and protein kinase G control a src-containing mechanosome in osteoblasts

Science Signaling

Volumn 3, Issue 153, 2010, Pages

  Rangaswami, Hema ^a   Schwappacher, Raphaela ^a   Marathe, Nisha ^a   Zhuang, Shunhui ^a   Casteel, Darren E ^a   Haas, Bodo ^b   Chen, Yong ^b   Pfeifer, Alexander ^b   Kato, Hisashi ^a   Shattil, Sanford ^a   Boss, Gerry R ^a   Pilz, Renate B ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BETA3 INTEGRIN; CYCLIC GMP; CYCLIC GMP DEPENDENT PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN KINASE G TYPE 2; PROTEIN TYROSINE KINASE; PROTEIN TYROSINE PHOSPHATASE SHP 1; PROTEIN TYROSINE PHOSPHATASE SHP 2; UNCLASSIFIED DRUG; BETA INTEGRIN; BROXURIDINE; NITRIC OXIDE;

ANIMAL CELL; ARTICLE; BONE; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; GENE EXPRESSION; MECHANOTRANSDUCTION; MOUSE; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; SHEAR STRESS; ANALYSIS OF VARIANCE; ANIMAL; BIOLOGICAL MODEL; BIOMECHANICS; CELL FRACTIONATION; CELL LINE; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; IMMUNOBLOTTING; IMMUNOPRECIPITATION; MECHANICAL STRESS; METABOLISM; MOUSE MUTANT; PHOSPHORYLATION; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SECOND MESSENGER;

MUS;

ANALYSIS OF VARIANCE; ANIMALS; BIOMECHANICS; BROMODEOXYURIDINE; CELL FRACTIONATION; CELL LINE; CELL PROLIFERATION; CYCLIC GMP; CYCLIC GMP-DEPENDENT PROTEIN KINASES; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOBLOTTING; IMMUNOPRECIPITATION; INTEGRIN BETA CHAINS; MECHANOTRANSDUCTION, CELLULAR; MICE; MICE, KNOCKOUT; MODELS, BIOLOGICAL; NITRIC OXIDE; OSTEOBLASTS; PHOSPHORYLATION; PROTEIN TYROSINE PHOSPHATASE, NON-RECEPTOR TYPE 11; PROTEIN TYROSINE PHOSPHATASE, NON-RECEPTOR TYPE 6; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SECOND MESSENGER SYSTEMS; SRC-FAMILY KINASES; STRESS, MECHANICAL;

EID: 78650674716     PISSN: 19450877     EISSN: 19379145     Source Type: Journal    
DOI: 10.1126/scisignal.2001423     Document Type: Article

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47. Acknowledgments: We are grateful to T. Diep, A. Fridman, and J. Meerloo for technical support, and to S. Ball, G. Firestein, and D. Boyle for providing operative bone specimens. Funding: This work was supported by NIH grants R01AR051300 (to R.B.P.), T32HL007261 (to N.M.), and P30NS047101 (UCSD Neuroscience Microscopy Shared Facility). Author contributions: H.R. performed BrdU uptake, fluid shear- and cGMP-induced Src/ERK phosphorylation, siRNA and viral reconstitution experiments, and with N.M. isolated hPOBs and performed RT-PCR. R.S. carried out the interaction and colocalization studies. S.Z. and D.E.C. performed site-directed mutagenesis, PTP activity assays, and phosphorylation studies. B.H., Y.C., and A.P. generated PKGIIdeficient mice and isolated mPOBs. H.K. prepared b3 integrin virus. R.B.P., H.R., R.S., G.R.B., and S.S. designed the experiments, analyzed the data, and wrote the manuscript. Competing interests: The authors declare that they have no competing interests. According to university policy, a materials transfer agreement (MTA) is required from R.B.P. for SHP-1, PKGI, or PKGII constructs and from A.P. for any materials related to the Prkg2-/- mice.