Characterization and study of the orientation of immobilized enzymes by tryptic digestion and HPLC-MS: Design of an efficient catalyst for the synthesis of cephalosporins

Biomacromolecules

Volumn 11, Issue 6, 2010, Pages 1623-1632

  Temporini, Caterina ^a,b   Bonomi, Paolo ^a,b   Serra, Immacolata ^a,b   Tagliani, Auro ^b,c   Bavaro, Teodora ^a,b   Ubiali, Daniela ^a,b   Massolini, Gabriella ^a,b   Terreni, Marco ^a,b  

^a UNIVERSITY OF PAVIA   (Italy)

^b Italian Biocatalysis Center   (Italy)

^c ACS Dobfar SpA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ALDEHYDES; CATALYSTS; ENZYMES; ESCHERICHIA COLI; FUNCTIONAL POLYMERS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MECHANICAL STABILITY; PEPTIDES; RESINS; SEWAGE TREATMENT PLANTS; SYNTHESIS (CHEMICAL);

ACTIVE SITE; AGAROSE; BINDING POCKETS; CATALYTIC PROPERTIES; CONTROLLED SYNTHESIS; EFFICIENT CATALYSTS; FUNCTIONALIZED; GLYOXYL; HPLC-MS; HPLC-MS ANALYSIS; HYDROPHOBIC RESIN; IMMOBILIZED ENZYME; INNOVATIVE APPROACHES; PENICILLIN G ACYLASE; REACTION MEDIUM; SOLID SUPPORTS; TRYPTIC DIGESTION;

DRUG PRODUCTS;

ACRYLIC ACID RESIN; ALDEHYDE DERIVATIVE; CEFACLOR; CEFAMANDOLE; CEFAZOLIN; CEFONICID; CEFPROZIL; CEPHALOSPORIN DERIVATIVE; EPOXY RESIN; EUPERGIT C; GLYOXYL AGAROSE; IMMOBILIZED ENZYME; LYSINE; MANDELYL 7 ACA; PENICILLIN AMIDASE; PROTEIN; RESIN; UNCLASSIFIED DRUG; GLYOXYLIC ACID DERIVATIVE; POLYMER; SEPHAROSE;

ARTICLE; BINDING AFFINITY; CATALYSIS; CATALYST; CONTROLLED STUDY; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; ENZYME KINETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; HYDROPHILICITY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN LOCALIZATION; TRYPSIN DIGESTION; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; ISOLATION AND PURIFICATION; KINETICS; POROSITY; SYNTHESIS; TANDEM MASS SPECTROMETRY;

CATALYSIS; CEPHALOSPORINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; GLYOXYLATES; KINETICS; MOLECULAR STRUCTURE; PENICILLIN AMIDASE; POLYMERS; POROSITY; SEPHAROSE; TANDEM MASS SPECTROMETRY;

EID: 77954585606     PISSN: 15257797     EISSN: 15264602     Source Type: Journal    
DOI: 10.1021/bm100259a     Document Type: Article

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