Improvement of catalytic properties of Escherichia coli penicillin G acylase immobilized on glyoxyl agarose by addition of a six-amino-acid tag

Applied and Environmental Microbiology

Volumn 71, Issue 12, 2005, Pages 8937-8940

  Scaramozzino, Francesca ^a,b   Estruch, Ilona ^a   Rossolillo, Paola ^a   Terreni, Marco ^a   Albertini, Alessandra M ^a  

^a UNIVERSITY OF PAVIA   (Italy)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; DERIVATIVES; ENZYME IMMOBILIZATION;

GLYCINES; IMMOBILIZATION EFFICIENCY; POSTTRANSLATIONAL STEPS; PROTEIN MATURATION;

ESCHERICHIA COLI;

AGAROSE; AMIDASE; AMINO ACID; GLYCINE; GLYOXYL AGAROSE; LYSINE; PENICILLIN G; PENICILLIN G ACYLASE; UNCLASSIFIED DRUG;

BIOTECHNOLOGY;

ARTICLE; BETA CHAIN; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CHEMICAL LABELING; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME MODIFICATION; GENE OVEREXPRESSION; NONHUMAN; PROTEIN PROCESSING;

BASE SEQUENCE; CATALYSIS; CLONING, MOLECULAR; DNA PRIMERS; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; INDICATORS AND REAGENTS; KINETICS; MUTAGENESIS, SITE-DIRECTED; OLIGOPEPTIDES; PENICILLIN AMIDASE; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 29144501578     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.12.8937-8940.2005     Document Type: Article

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