Valine 375 and phenylalanine 109 confer affinity and specificity for pyruvate as donor substrate in acetohydroxy acid synthase isozyme II from escherichia coli

Biochemistry

Volumn 49, Issue 25, 2010, Pages 5188-5199

  Steinmetz, Andrea ^a   Vyazmensky, Maria ^b   Meyer, Danilo ^a   Barak, Zeev ^b   Golbik, Ralph ^c   Chipman, David M ^b   Tittmann, Kai ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

^c MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACETOHYDROXY ACID SYNTHASE; COFACTORS; DONOR SUBSTRATES; HYDROPHOBIC INTERACTIONS; IN-PLANTS; MOLECULAR DETERMINANTS; MULTIPLE TRANSITIONS; PYRUVATES; SEQUENCE ALIGNMENTS; SIDE CHAINS; STEADY STATE; STERIC CONSTRAINT; STOPPED-FLOW KINETICS; STRUCTURAL STUDIES; SUBSTRATE AFFINITY; SUBSTRATE BINDING; TETRAHEDRAL INTERMEDIATES; THERMODYNAMIC STUDIES; THIAMIN DIPHOSPHATE-DEPENDENT ENZYMES; UNIMOLECULAR; WILD TYPES;

ALIGNMENT; AMINO ACIDS; BIOCHEMISTRY; CIRCULAR DICHROISM SPECTROSCOPY; DICHROISM; ENZYMES; ESCHERICHIA COLI; HYDROPHOBICITY; ORGANIC ACIDS;

SUBSTRATES;

ACETOLACTATE SYNTHASE; ENZYME VARIANT; ISOENZYME; PHENYLALANINE; PYRUVIC ACID; VALINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; ENZYME KINETICS; ESCHERICHIA COLI; HUMAN; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PIR-INTERNATIONAL PROTEIN SEQUENCE DATABASE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN STRUCTURE;

ACETOLACTATE SYNTHASE; AMINO ACID SEQUENCE; CIRCULAR DICHROISM; ESCHERICHIA COLI; ISOENZYMES; KINETICS; MOLECULAR SEQUENCE DATA; PHENYLALANINE; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; THERMODYNAMICS; VALINE;

ALGAE; ESCHERICHIA COLI; FUNGI;

EID: 77953880857     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi100555q     Document Type: Article

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