Crystal structure of yeast acetohydroxyacid synthase: A target for herbicidal inhibitors

Journal of Molecular Biology

Volumn 317, Issue 2, 2002, Pages 249-262

  Pang, Siew Siew ^a   Duggleby, Ronald G ^a   Guddat, Luke W ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Acetohydroxyacid synthase; Acetolactate synthase; FAD; Herbicide inhibition; Thiamin diphosphate

Indexed keywords

ACETOLACTATE SYNTHASE; DIMER; FLAVINE ADENINE NUCLEOTIDE; HERBICIDE;

ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME CONFORMATION; ENZYME STRUCTURE; ENZYME SUBUNIT; NONHUMAN; PRIORITY JOURNAL; YEAST;

EID: 0036295208     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.5419     Document Type: Article

References (50)

1. Biosynthesis of 2-aceto-2-hydroxy acids: Acetolactate synthases and acetohydroxyacid synthases
2. Acetohydroxyacid synthase
3. Structure of the thiamine- and flavin-dependent enzyme pyruvate oxidase
4. Catalytic centers in the thiamin diphosphate dependent enzyme pyruvate decarboxylase at 2.4-Å resolution
5. Crystal structure of the thiamin diphosphate-dependent enzyme pyruvate decarboxylase from yeast Saccharromyces cerevisiae at 2.3 Å resolution
6. High resolution crystal structure of pyruvate decarboxylase from Zymomonas mobilis. Implications for substrate activation in pyruvate decarboxylases
7. The crystal structure of benzoylformate decarboxylase at 1.6 Å resolution: Diversity of catalytic residues in thiamin diphosphate-dependent enzymes
8. Common ancestry of Escherichia coli pyruvate oxidase and the acetohydroxy acid synthases of the branched-chain amino acid biosynthetic pathway
9. Acetohydroxyacid synthase inhibitors
10. Imidazolinones: Potent inhibitors of acetohydroxyacid synthase
11. Origin of the herbicide binding site of acetolactate synthase
12. New aspects on inhibition of plant acetolactate synthase by chlorsulfuron and imazaquin
13. Purification of Escherichia coli acetohydroxyacid synthase isozyme II and reconstitution of active enzyme from its individual pure subunits
14. Expression, purification and characterization of Arabidopsis thaliana acetohydroxyacid synthase
15. Homology modeling of the structure of bacterial acetohydroxy acid synthase and examination of the active site by site-directed mutagenesis
16. Rational molecular design and genetic engineering of herbicide resistant crops by structure modeling and site-directed mutagenesis of acetohydroxyacid synthase
17. Expression, purification, characterization and reconstitution of the large and small subunits of yeast acetohydroxyacid synthase
18. Regulation of yeast acetohydroxyacid synthase by valine and ATP
19. Crystallization of the catalytic subunit of Saccharo-myces cerevisiae acetohydroxyacid synthase
20. Three-dimensional structure of transketolase, a thiamine diphosphate dependent enzyme, at 2.5 Å resolution
21. Binding and activation of thiamin diphosphate in acetohydroxyacid synthase
22. Analysis of an invariant cofactor-protein interaction in thiamin diphosphate-dependent enzymes by site-directed mutagenesis - Glutamic-acid-418 in transketolase is essential for catalysis
23. The role of residues glutamate-50 and phenylalanine-496 in Zymomonas mobilis pyruvate decarboxylase
24. Role of Glu51 for cofactor binding and catalytic activity in pyruvate decarboxylase from yeast studied by site-directed mutagenesis
25. Identification of the catalytic glutamate in the E1 component of human pyruvate dehydrogenase
26. Effects of deletions at the carboxyl terminus of Zymomonas mobilis pyruvate decarboxylase on the kinetic properties and substrate specificity
27. Purification and properties of Saccharomyces cerevisiae acetolactate synthase from recombinant Escherichia coli
28. The refined structures of a stabilized mutant and of wild-type pyruvate oxidase from Lactobacillus plantarum
29. Flavoenzymes: Diverse catalysts with recurrent features
30. Acetolactate synthase and ketol-acid reductoisomerase: A search for a reason and a reason for a search
31. Stability and reconstitution of pyruvate oxidase from Lactobacillus plantarum: Dissection of the stabilizing effects of coenzyme binding and subunit interactions
32. Role of flavin in acetoin production by two bacterial pyruvate oxidases
33. Activation of thiamin diphosphate and FAD in the phosphate-dependent pyruvate oxidase from Lactobacillus plantarum
34. The pH 6 acetolactate-forming enzyme from Aerobacter aerogenes. II. Evidence that it is not a flavoprotein
35. Engineering herbicide-resistant acetolactate synthase
36. The development of herbicide resistant crops
37. Development and validation of a genetic algorithm for flexible docking
38. Aspartate-27 and glutamate-473 are involved in catalysis by Zymomonas mobilis pyruvate decarboxylase
39. Site-directed mutagenesis of the ionizable groups in the active site of Zymomonas mobilis pyruvate decarboxylase: Effect on activity and pH dependence
40. Catalytic acid-base groups in yeast pyruvate decarboxylase. 2. Insights into the specific roles of D28 and E477 from the rates and stereospecificity of formation of carboligase side products
41. Processing of X-ray diffraction data collected in oscillation mode
42. AMoRe: An automated package for molecular replacement
43. Crystallography and NMR System (CNS): A new software system for macromolecular structure determination
44. Improved methods for building protein models in electron density maps and the location of errors in these models
45. LIGPLOT: A program to generate schematic diagrams of protein-ligand interactions
46. SETOR: Hardware lighted three dimensional solid model representations of macromolecules
47. MOLSCRIPT - A program to produce both detailed and schematic plots of protein structures
48. Raster3D version 2.01: A program for photorealistic molecular graphics
49. ESPript: Analysis of multiple sequence alignments in PostScript
50. A common structural motif in thiamin pyrophosphate-binding enzymes