A thiamin-bound, pre-decarboxylation reaction intermediate analogue in the pyruvate dehydrogenase E1 subunit induces large scale disorder-to-order transformations in the enzyme and reveals novel structural features in the covalently bound adduct

Journal of Biological Chemistry

Volumn 281, Issue 22, 2006, Pages 15296-15303

  Arjunan, Palaniappa ^a,b   Sax, Martin ^a   Brunskill, Andrew ^a,b   Chandrasekhar, Krishnamoorthy ^a,b   Nemeria, Natalia ^c   Zhang, Sheng ^c   Jordan, Frank ^c   Furey, William ^a,b  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^c RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEXATION; CRYSTAL STRUCTURE; ENZYME KINETICS; HYDROGEN BONDS; MOLECULAR STRUCTURE; REACTION KINETICS;

DISORDER-TO-ORDER TRANSFORMATIONS; PYRUVATE DEHYDROGENASE; STRUCTURAL FEATURES; THIAMIN;

ENZYMES;

PYROPHOSPHATE; PYRUVATE DEHYDROGENASE; PYRUVATE DEHYDROGENASE E1 ENZYME; THIAMINE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; DECARBOXYLATION; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME MECHANISM; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION;

AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; ELECTROSTATICS; ESCHERICHIA COLI; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, QUATERNARY; PROTEIN SUBUNITS; PYRUVATE DEHYDROGENASE COMPLEX; THIAMINE;

ESCHERICHIA COLI;

EID: 33646859093     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M600656200     Document Type: Article

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