Structural and kinetic studies on native intermediates and an intermediate analogue in benzoylformate decarboxylase reveal a least motion mechanism with an unprecedented short-lived predecarboxylation intermediate

Biochemistry

Volumn 48, Issue 15, 2009, Pages 3258-3268

  Bruning, Marc ^a   Berheide, Marco ^a   Meyer, Danilo ^b,c   Golbik, Ralph ^c   Bartunik, Hans ^d   Liese, Andreas ^a   Tittmann, Kai ^b,c  

^a HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

^c MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^d MAX PLANCK UNIT FOR STRUCTURAL MOLECULAR BIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE CENTERS; AMINO GROUPS; BINDING MECHANISMS; CARBONYL ADDITIONS; CD SPECTROSCOPIES; CHROMOPHORIC SUBSTRATES; CO CRYSTALLIZATIONS; COFACTOR; DECARBOXYLASE; FREE ACIDS; HYDROPHOBIC POCKETS; IN LINES; KINETIC ANALYSIS; KINETIC STUDIES; METHYL ESTERS; MOTION MECHANISMS; NON-COVALENT COMPLEXES; NON-OXIDATIVE; OH GROUPS; ORDER OF MAGNITUDES; OUT OF PLANES; PHENYL RINGS; PHOSPHONATE; PSEUDOMONAS PUTIDA; REACTION INTERMEDIATES; STEADY-STATE DISTRIBUTIONS; STOPPED-FLOW KINETICS; STRUCTURAL INFORMATIONS; STRUCTURAL REORGANIZATIONS; SUBSTRATE SATURATIONS; THIAMIN DIPHOSPHATE; X-RAY STRUCTURES;

ACIDS; ALDEHYDES; AMINES; CARBON DIOXIDE; CARBONYLATION; CARBOXYLATION; ENZYMES; ESTERIFICATION; ESTERS; HYDROGEN; HYDROGEN BONDS; SUBSTRATES;

RATE CONSTANTS;

BENZALDEHYDE; BENZOYLFORMATE DECARBOXYLASE; CARBONYL DERIVATIVE; COCARBOXYLASE; HISTIDINE DERIVATIVE; LEUCINE; MANDELIC ACID; PHENYLALANINE; PHENYLALANINE DERIVATIVE; PHENYLGLYOXYLIC ACID; PHOSPHONIC ACID DERIVATIVE; PHOSPHONIC ACID ESTER; SERINE; SERINE DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CIRCULAR DICHROISM; CONTROLLED STUDY; COVALENT BOND; CRYSTAL STRUCTURE; DECARBOXYLATION; ELIMINATION REACTION; ENZYME ASSAY; ENZYME BINDING; ENZYME CONFORMATION; ENZYME MECHANISM; ENZYME STRUCTURE; HYDROGEN BOND; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTON NUCLEAR MAGNETIC RESONANCE; PSEUDOMONAS PUTIDA; REACTION TIME; STEADY STATE; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

BACTERIAL PROTEINS; CARBOXY-LYASES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; DECARBOXYLATION; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; PSEUDOMONAS PUTIDA; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; THERMODYNAMICS; TIME FACTORS;

PSEUDOMONAS PUTIDA;

EID: 65249164076     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi801957d     Document Type: Article

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