Homology modeling of the structure of bacterial acetohydroxy acid synthase and examination of the active site by site-directed mutagenesis

Biochemistry

Volumn 35, Issue 50, 1996, Pages 16282-16291

  Ibdah, Muhammad ^a   Bar Ilan, Ahuva ^a   Livnah, Oded ^b,d   Schloss, John V ^c   Barak, Ze'ev ^a   Chipman, David M ^a  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^c UNIVERSITY OF KANSAS   (United States)

^d SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 OXOBUTYRIC ACID; ACETOLACTATE SYNTHASE;

ARTICLE; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; MODEL; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

EID: 0030468649     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi961588i     Document Type: Article

References (52)

1. Arjunan, P., Umland, T., Dyda, F., Swaminathan, S., Furey, W., Sax, M., Farrenkopf, B., Gao, Y., Zhang, D., & Jordan, F. (1996) J. Mol. Biol. 256, 590-600.
2. Barak, Z., Chipman, D. M., & Gollop, N. (1987) J. Bacteriol. 169, 3750-3756.
3. Bernasconi, P., Woodworth, A. R., Rosen, B. A., Subramanian, M. V., & Siehl, D. L. (1995) J. Biol Chem. 270, 17381-17385.
4. Bowie, J. U., Lüthy, R., & Eisenberg, D. (1991) Science 253, 164-170.
5. Breslow, R. (1958) J. Am. Chem. Soc. 80, 3719-3726.
6. Chang, Y.-Y., & Cronan, J. E., Jr. (1988) J. Bacteriol. 170, 3937-3945.
7. Chang, Y.-Y., Wang, A. Y., & Cronan, J. E., Jr. (1993) J. Biol. Chem. 268, 3911-3919.
8. Cohen-Fix, O., & Livneh, Z. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 3300-3304.
9. Conway, T., Osman, Y., Konnan, J., Hoffmann, E., & Ingram, L. O. (1987) J. Bacterial. 169, 949-954.
10. Eoyang, L., & Silverman, P. M. (1986) J. Bacteriol. 166, 901-904.
11. Falco, S. C., Dumas, K. S., & Livak, K. J. (1985) Nucleic Acids Res. 13, 4011-4027.
12. Fothergill-Gilmore, L. A., & Watson, H. C. (1989) Adv. Enzymol. Relat. Areas Mol. Biol. 62, 227-313.
13. Friden, P., Donegan, J., Mullen, J., Tsui, P., Freundlich, M., Eoyang, L., Weber, R., & Silverman, P. M. (1985) Nucleic Acids Res. 13, 3979-3993.
14. Genetics Computer Group (1991) Program manual for the GCG package, Version 7, Genetics Computer Group, Madison, WI.
15. Godon, J.-J., Chopin, M.-C. M., & Ehrlich, D. S. (1992) J. Bacteriol. 174, 6580-6589.
16. Gollop, N., Damri, B., Barak, Z., & Chipman, D. M. (1989) Biochemistry 28, 6310-6317.
17. Gollop, N., Damri, B., Chipman, D. M., & Barak, Z. (1990) J. Bacteriol. 172, 3444-3449.
18. Grabau, C., & Cronan, J. E., Jr. (1986) Nucleic Acids Res. 14, 5449-5460.
19. Green, J. B. (1989) FEBS Lett. 246, 1-5.
20. Harms, E., Hsu, J. H., Subrahmanayam, C. S., & Umbarger, H. E. (1985) J. Bacteriol. 164, 207-216.
21. Hattori, J., Brown, D., Mourad, G., Labbe, H., Quellet, T., Sunohara, G., Rutledge, R., King, J., & Miki, B. (1995) Mol. Gen. Genet. 246, 419-425.
22. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K., & Pease, L. R. (1989) Gent 77, 51-59.
23. Inui, M., Vertes, A. A., Kobayashi, M., Kurusu, Y., & Yukawa, H. (1993) DNA Sequence 3, 303-310.
24. Jones, T. A. (1978) J. Appl. Crystallogr. 11, 268-272.
25. Jones, T. A., Zhou, J.-Y., & Cowan, S. W. (1991) Acta Crystallogr. A47, 110-119.
26. Kepner, E. S. (1993) FEBS Lett. 326, 4-10.
27. Kluger, R. (1987) Chem. Rev. 87, 863-876.
28. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950.
29. Lawther, R. P., Calhoun, D. H., Adams, C. W., Hauser, C. A., Gray, J., & Hatfield, G. W. (1981) Proc, Natl. Acad. Sci. U.S.A. 78, 922-925.
30. Lee, K. Y., Townsend, J., Tepperman, J., Black, M., Chui, C. F., Mazur, B., Dunsmuir, P., & Bedbrook, J. (1988) EMBO J. 7, 1241-1248.
31. Lindqvist, Y., Schneider, G., Ermler, U., & Sundstrom, M. (1992) EMBO J. 11, 2373-2379.
32. Lorimer, G. H., Chen, Y.-R., & Hartman, F. C. (1993) Biochemistry 32, 9018-9024.
33. Lu, M. F., & Umbarger, H. E. (1987) J. Bacteriol. 169, 600-604.
34. Mazur, B., & Falco, S. C. (1989) Annu. Rev. Plant Physiol. 40, 441-470.
35. Muller, Y. A., & Schulz, G. E. (1993) Science 259, 965-967.
36. Muller, Y. A., Lindqvist, Y., Furey, W., Schulz, G. E., Jordan, F., & Schneider, G. (1993) Structure 1, 95-103.
37. Muller, Y. A., Schumacher, G., Rudolph, R., & Schulz, G. E. (1994) J. Mol. Biol. 237, 315-335.
38. Pompliano, D. L., Peyman, A., & Knowles, J. R. (1990) Biochemistry 29, 3186-3194.
39. Reith, M., & Munholland, J. (1993) Curr. Genet. 23, 59-65.
40. Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989) Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Press, Plainview, NY.
41. Sathasivan, K., Haughn, G. W., & Murai, N. (1990) Nucleic Acids Res. 18, 2188.
42. Sathasivan, K., Haughn, G. W., & Murai, N. (1991) Plant Physiol. 97, 1044-1050.
43. Schloss, J. V., & Aulabaugh, A. (1990) in Biosynthesis of Branched Chain Amino Acids (Barak, Z., Chipman, D. M., & Schloss, J. V., Eds.) pp 329-356, VCH, Weinheim, Germany.
44. Schloss, J. V., Ciskanik, L. M., & VanDyk, D. E. (1988) Nature 331, 360-362.
45. Sella, C., Weinstock, O., Chipman, D. M., & Barak, Z. (1993) J. Bacteriol. 175, 5339-5343.
46. Squires, C. H., DeFelice, M., Devereux, J., & Calvo, J. M. (1983) Nucleic Acids Res. 11, 5299-5313.
47. Tse, J. M. T., & Schloss, J. V. (1993) Biochemistry 32, 10398-10403.
48. Umbarger, H. E. (1987) in Escherichia coli and Salmonella typhimurium. Cellular and molecular biology (Neidhardt, F. C., Ingraham, J. L., Low, B. L., Magasanik, B., Schaechter, M., & Umbarger, H. E., Eds.) pp 352-367, American Society for Microbiology, Washington, DC.
49. Vyazmensky, M., Sella, C., Barak, Z., & Chipman, D. M. (1996) Biochemistry 35, 10339-10346.
50. Weinstock, O., Sella, C., Chipman, D. M., & Barak, Z. (1992) J. Bacteriol. 174, 5560-5566.
51. Winn, S. I., Watson, H. C., Harkins, R. N., & Fothergill, L. A. (1981) Philos. Trans. R. Soc. London B293, 121-130.
52. Yadav, N., McDevitt, R., Benard, S., & Falco, S. C. (1986) Proc. Nail. Acad. Sci. U.S.A. 83, 4418-4422.