The use of native cation-exchange chromatography to study aggregation and phase separation of monoclonal antibodies

Protein Science

Volumn 19, Issue 6, 2010, Pages 1191-1204

  Chen, Shuang ^a,b   Lau, Hollis ^a   Brodsky, Yan ^a   Kleemann, Gerd R ^a   Latypov, Ramil F ^a  

^a AMGEN INC   (United States)

^b PURDUE UNIVERSITY   (United States)

Author keywords

Antibody aggregation; Antibody phase separation; Cation exchange chromatography; Fab and Fc fragments; Limited proteolysis; Monoclonal antibody

Indexed keywords

FATTY ACID; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G1 ANTIBODY; IMMUNOGLOBULIN G2; MONOCLONAL ANTIBODY;

ANTIBODY ENGINEERING; ARTICLE; GEL PERMEATION CHROMATOGRAPHY; HIGH TEMPERATURE; ION EXCHANGE CHROMATOGRAPHY; PH; PHASE SEPARATION; PHYSICAL STRESS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN PROTEIN INTERACTION;

ANTIBODIES, MONOCLONAL; CALORIMETRY, DIFFERENTIAL SCANNING; CATIONS; CHROMATOGRAPHY, ION EXCHANGE; FATTY ACIDS; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; PROTEIN MULTIMERIZATION; TEMPERATURE;

EID: 77952684807     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.396     Document Type: Article

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