Physical instability of a therapeutic Fc fusion protein: Domain contributions to conformational and colloidal stability

Biochemistry

Volumn 48, Issue 49, 2009, Pages 11724-11736

  Fast, Jonas L ^a,c   Cordes, Amanda A ^a   Carpenter, John F ^b   Randolph, Theodore W ^a  

^a University of Colorado at Boulder   (United States)

^b UNIVERSITY OF COLORADO   (United States)

^c F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ACCELERATED DEGRADATION; AGGREGATION KINETICS; BIOPHARMACEUTICALS; CHAOTROPES; CIRCULAR DICHROISM; COLLOIDAL STABILITY; CONFORMATIONAL INSTABILITY; CONFORMATIONAL STABILITIES; ELECTROSTATIC POTENTIALS; EXPERIMENTAL TECHNIQUES; FUSION PROTEINS; INTRINSIC FLUORESCENCE; MOLTEN GLOBULE; PH-DEPENDENT; PROTEIN DOMAINS; PROTEIN THERAPEUTICS; REACTION PATHWAYS; SECOND VIRIAL COEFFICIENTS; T-CELL RECEPTORS;

CELL MEMBRANES; CHROMATOGRAPHIC ANALYSIS; DICHROISM; DIFFERENTIAL SCANNING CALORIMETRY; EQUATIONS OF STATE; SIZE EXCLUSION CHROMATOGRAPHY; STABILITY; ZETA POTENTIAL;

PROTEINS;

ABATACEPT; CYTOTOXIC T LYMPHOCYTE ANTIGEN 4; HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT;

ARTICLE; CHEMICAL REACTION; CIRCULAR DICHROISM; COLLOID; DIFFERENTIAL SCANNING CALORIMETRY; DRUG CONFORMATION; DRUG STABILITY; ELECTRIC POTENTIAL; FLUORESCENCE; GEL PERMEATION CHROMATOGRAPHY; LUMRY EYRING REACTION; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN STABILITY; TEMPERATURE MEASUREMENT; ZETA POTENTIAL;

AMINO ACID SEQUENCE; COLLOIDS; CRYSTALLOGRAPHY, X-RAY; HUMANS; IMMUNOCONJUGATES; IMMUNOGLOBULIN FC FRAGMENTS; LIGHT; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SCATTERING, RADIATION;

EID: 71549161144     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi900853v     Document Type: Article

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