Impact of coexpression and coamplification of sICAM and antiapoptosis determinants bcl-2/bcl-xL on productivity, cell survival, and mitochondria number in CHO-DG44 grown in suspension and serum-free media

Biotechnology and Bioengineering

Volumn 80, Issue 6, 2002, Pages 706-716

  Meents, Heiko ^a,b   Enenkel, Barbara ^b   Eppenberger, Hans M ^a   Werner, Rolf G ^b   Fussenegger, Martin ^a  

^a ETH ZURICH   (Switzerland)

^b BOEHRINGER INGELHEIM PHARMA GMBH AND CO KG   (Germany)

Author keywords

Apoptosis; Bcl 2; Bcl xL; Dhfr; Mitochondria content; Serum free

Indexed keywords

ADHESION; CELL CULTURE; VIRUSES;

CELL LINES;

BIOTECHNOLOGY;

DIHYDROFOLATE REDUCTASE; INTERCELLULAR ADHESION MOLECULE 1; PROTEIN BCL 2; PROTEIN BCL XL;

ADAPTATION; ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CULTURE; CELL DEATH; CELL GROWTH; CELL PROTECTION; CELL SURVIVAL; CHO CELL; CISTRON; COMMON COLD; CONTROLLED STUDY; CULTURE MEDIUM; ENCEPHALOMYOCARDITIS VIRUS; GENE AMPLIFICATION; GENE OVEREXPRESSION; GENETIC TRANSFECTION; INTERNAL RIBOSOME ENTRY SITE; MITOCHONDRION; NONHUMAN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SUSPENSION; TRANSLATION INITIATION; ANIMAL; COMPARATIVE STUDY; CYTOLOGY; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETIC ENHANCEMENT; GENETICS; HAMSTER; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PHYSIOLOGY; REFERENCE VALUE; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; ULTRASTRUCTURE;

CRICETINAE; CRICETULUS GRISEUS; ENCEPHALOMYOCARDITIS VIRUS;

ANIMALS; APOPTOSIS; CELL SURVIVAL; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CULTURE MEDIA, SERUM-FREE; GENE AMPLIFICATION; GENE EXPRESSION REGULATION; GENETIC ENHANCEMENT; INTERCELLULAR ADHESION MOLECULE-1; MITOCHONDRIA; PROTO-ONCOGENE PROTEINS C-BCL-2; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; SUSPENSIONS; TRANSFECTION;

EID: 0037146823     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10449     Document Type: Article

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