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Volumn 21, Issue 1, 2010, Pages 144-153

Ion Trap Collision-Induced Dissociation of Locked Nucleic Acids

Author keywords

[No Author keywords available]

Indexed keywords

CHARGE STATE; COLLISION-INDUCED DISSOCIATION; DIFFERENT PRECURSORS; DISSOCIATION CHANNELS; DNA NUCLEOTIDES; FRAGMENT IONS; GAS-PHASE DISSOCIATION; GLYCOSIDIC BOND; ION CHARGE STATE; ION TRAPS; LOCKED NUCLEIC ACID; RELATIVE CONTRIBUTION; SEQUENCE CHARACTERIZATION; TANDEM MASS SPECTROMETRY;

EID: 73449139069     PISSN: 10440305     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jasms.2009.09.020     Document Type: Article
Times cited : (22)

References (48)
  • 1
    • 3242677639 scopus 로고    scopus 로고
    • SiRNAs: Applications in Functional Genomics and Potential as Therapeutics
    • Dorsett Y., and Tuschl T. SiRNAs: Applications in Functional Genomics and Potential as Therapeutics. Nat. Rev. Drug Discov 3 (2004) 318-329
    • (2004) Nat. Rev. Drug Discov , vol.3 , pp. 318-329
    • Dorsett, Y.1    Tuschl, T.2
  • 2
    • 60149088848 scopus 로고    scopus 로고
    • Origins and Mechanisms of miRNAs and siRNAs
    • Carthew R.W., and Sontheimer E.J. Origins and Mechanisms of miRNAs and siRNAs. Cell 136 (2009) 642-655
    • (2009) Cell , vol.136 , pp. 642-655
    • Carthew, R.W.1    Sontheimer, E.J.2
  • 3
    • 33747876113 scopus 로고    scopus 로고
    • MicroRNA-Cancer Connection: The Beginning of a New Tale
    • Calin G.A., and Croce C.M. MicroRNA-Cancer Connection: The Beginning of a New Tale. Cancer Res 66 (2006) 7390-7394
    • (2006) Cancer Res , vol.66 , pp. 7390-7394
    • Calin, G.A.1    Croce, C.M.2
  • 4
    • 60149084560 scopus 로고    scopus 로고
    • MicroRNAs and Cancer: Short RNAs Go a Long Way
    • Ventura A., and Jacks T. MicroRNAs and Cancer: Short RNAs Go a Long Way. Cell 136 (2009) 586-591
    • (2009) Cell , vol.136 , pp. 586-591
    • Ventura, A.1    Jacks, T.2
  • 6
    • 0035195942 scopus 로고    scopus 로고
    • Noncoding RNAs: The Architects of Eukaryotic Complexity
    • Mattick J.S. Noncoding RNAs: The Architects of Eukaryotic Complexity. EMBO Rep 2 (2001) 986-991
    • (2001) EMBO Rep , vol.2 , pp. 986-991
    • Mattick, J.S.1
  • 7
    • 24644443227 scopus 로고    scopus 로고
    • The Functional Genomics of Noncoding RNA
    • Mattick J.S. The Functional Genomics of Noncoding RNA. Science 309 (2005) 1527-1528
    • (2005) Science , vol.309 , pp. 1527-1528
    • Mattick, J.S.1
  • 9
    • 33747706117 scopus 로고    scopus 로고
    • Noncoding RNAs in the Nervous System
    • Mehler M.F., and Mattick J.S. Noncoding RNAs in the Nervous System. J. Physiol 575 (2006) 333-341
    • (2006) J. Physiol , vol.575 , pp. 333-341
    • Mehler, M.F.1    Mattick, J.S.2
  • 10
    • 36749037521 scopus 로고    scopus 로고
    • The Piwi-piRNA Pathway Provides an Adaptive Defense in the Transposon Arms Race
    • Aravin A.A., Hannon G.J., and Brennecke J. The Piwi-piRNA Pathway Provides an Adaptive Defense in the Transposon Arms Race. Science 318 (2007) 761-764
    • (2007) Science , vol.318 , pp. 761-764
    • Aravin, A.A.1    Hannon, G.J.2    Brennecke, J.3
  • 11
    • 38649102786 scopus 로고    scopus 로고
    • Behind the Scenes of a Small RNA Gene-Silencing Pathway
    • Ku G., and McManus M.Y. Behind the Scenes of a Small RNA Gene-Silencing Pathway. Hum. Gene Ther 19 (2008) 17-26
    • (2008) Hum. Gene Ther , vol.19 , pp. 17-26
    • Ku, G.1    McManus, M.Y.2
  • 12
    • 0041331496 scopus 로고    scopus 로고
    • SiRNA Function in RNAi: A Chemical Modification Analysis
    • Chiu Y.L., and Rana T.M. SiRNA Function in RNAi: A Chemical Modification Analysis. RNA 9 (2003) 1034-1048
    • (2003) RNA , vol.9 , pp. 1034-1048
    • Chiu, Y.L.1    Rana, T.M.2
  • 13
    • 8844262560 scopus 로고    scopus 로고
    • RNA Interference and Chemically Modified Small Interfering RNAs
    • Manoharan M. RNA Interference and Chemically Modified Small Interfering RNAs. Curr. Opin. Chem. Biol 8 (2004) 570-579
    • (2004) Curr. Opin. Chem. Biol , vol.8 , pp. 570-579
    • Manoharan, M.1
  • 14
    • 0343065617 scopus 로고    scopus 로고
    • LNA (Locked Nucleic Acids): Synthesis and High-Affinity Nucleic Acid Recognition
    • Singh S.K., Nielsen P., Koshkin A.A., and Wengel J. LNA (Locked Nucleic Acids): Synthesis and High-Affinity Nucleic Acid Recognition. Chem. Commun 4 (1998) 455-456
    • (1998) Chem. Commun , vol.4 , pp. 455-456
    • Singh, S.K.1    Nielsen, P.2    Koshkin, A.A.3    Wengel, J.4
  • 15
    • 0032473890 scopus 로고    scopus 로고
    • LNA (Locked Nucleic Acids): Synthesis of the Adenine, Cytosine, Guanine, 5-Methylcytosine, Thymine, and Uracil Bicyclonucleoside Monomers, Oligomerization, and Unprecedented Nucleic Acid Recognition
    • Koshkin A.A., Singh S.K., Nielsen P., Rajwanshi V.K., Kumar R., Meldgaard M., Olsen C.E., and Wengel J. LNA (Locked Nucleic Acids): Synthesis of the Adenine, Cytosine, Guanine, 5-Methylcytosine, Thymine, and Uracil Bicyclonucleoside Monomers, Oligomerization, and Unprecedented Nucleic Acid Recognition. Tetrahedron 54 14 (1998) 3607-3630
    • (1998) Tetrahedron , vol.54 , Issue.14 , pp. 3607-3630
    • Koshkin, A.A.1    Singh, S.K.2    Nielsen, P.3    Rajwanshi, V.K.4    Kumar, R.5    Meldgaard, M.6    Olsen, C.E.7    Wengel, J.8
  • 17
    • 0036606669 scopus 로고    scopus 로고
    • Design and Characterization of Decoy Oligonucleotides Containing Locked Nucleic Acids
    • Crinelli R., Bianchi M., Gentilini L., and Magnani M. Design and Characterization of Decoy Oligonucleotides Containing Locked Nucleic Acids. Nucleic Acids Res 30 11 (2002) 2435-2443
    • (2002) Nucleic Acids Res , vol.30 , Issue.11 , pp. 2435-2443
    • Crinelli, R.1    Bianchi, M.2    Gentilini, L.3    Magnani, M.4
  • 18
    • 0037143606 scopus 로고    scopus 로고
    • Oligonucleotide Directed Misfolding of RNA Inhibits Candida Albicans Group I Intron Splicing
    • Childs J.L., Disney M.D., and Turner D.H. Oligonucleotide Directed Misfolding of RNA Inhibits Candida Albicans Group I Intron Splicing. Proc. Natl. Acad. Sci. U.S.A 99 17 (2002) 11091-11096
    • (2002) Proc. Natl. Acad. Sci. U.S.A , vol.99 , Issue.17 , pp. 11091-11096
    • Childs, J.L.1    Disney, M.D.2    Turner, D.H.3
  • 19
    • 42349107713 scopus 로고    scopus 로고
    • Nucleic Acid Beacons for Long-Term Real-Time Intracellular Monitoring
    • Wu Y.R., Yang C.J., Moroz L.L., and Tan W.H. Nucleic Acid Beacons for Long-Term Real-Time Intracellular Monitoring. Anal. Chem 80 8 (2008) 3025-3028
    • (2008) Anal. Chem , vol.80 , Issue.8 , pp. 3025-3028
    • Wu, Y.R.1    Yang, C.J.2    Moroz, L.L.3    Tan, W.H.4
  • 20
    • 66149123741 scopus 로고    scopus 로고
    • Direct Detection of Abortive RNA Transcripts In Vivo
    • Goldman S.R., Ebright R.H., and Nickels B.E. Direct Detection of Abortive RNA Transcripts In Vivo. Science 324 5929 (2009) 927-928
    • (2009) Science , vol.324 , Issue.5929 , pp. 927-928
    • Goldman, S.R.1    Ebright, R.H.2    Nickels, B.E.3
  • 21
    • 34147180058 scopus 로고    scopus 로고
    • Evaluation of Locked Nucleic Acid-Modified Small Interfering RNA In Vitro and In Vivo
    • Mook O.R., Baas F., de Wissel M.B., and Fluiter K. Evaluation of Locked Nucleic Acid-Modified Small Interfering RNA In Vitro and In Vivo. Mol. Cancer Ther 6 3 (2007) 833-843
    • (2007) Mol. Cancer Ther , vol.6 , Issue.3 , pp. 833-843
    • Mook, O.R.1    Baas, F.2    de Wissel, M.B.3    Fluiter, K.4
  • 22
    • 2942631142 scopus 로고    scopus 로고
    • LNA/DNA Chimeric Oligomers Mimic RNA Aptamers Targeted to the TAR RNA Element of HIV-1
    • Darfeuille F., Hansen J.B., Orum H., Di Primo C., and Toulme J.J. LNA/DNA Chimeric Oligomers Mimic RNA Aptamers Targeted to the TAR RNA Element of HIV-1. Nucleic Acids Res 32 10 (2004) 3101-3107
    • (2004) Nucleic Acids Res , vol.32 , Issue.10 , pp. 3101-3107
    • Darfeuille, F.1    Hansen, J.B.2    Orum, H.3    Di Primo, C.4    Toulme, J.J.5
  • 24
    • 33748323477 scopus 로고    scopus 로고
    • Antisense Locked Nucleic Acids Efficiently Suppress BCR/ABL and Induce Cell Growth Decline and Apoptosis in Leukemic Cells
    • Rapozzi V., Cogoi S., and Xodo L.E. Antisense Locked Nucleic Acids Efficiently Suppress BCR/ABL and Induce Cell Growth Decline and Apoptosis in Leukemic Cells. Mol. Cancer Ther 5 7 (2006) 1683-1692
    • (2006) Mol. Cancer Ther , vol.5 , Issue.7 , pp. 1683-1692
    • Rapozzi, V.1    Cogoi, S.2    Xodo, L.E.3
  • 25
    • 3342961932 scopus 로고    scopus 로고
    • LNA-Antisense Rivals siRNA for Gene Silencing
    • Jepsen J.S., and Wengel J. LNA-Antisense Rivals siRNA for Gene Silencing. Curr. Opin. Drug Discov 7 2 (2004) 188-194
    • (2004) Curr. Opin. Drug Discov , vol.7 , Issue.2 , pp. 188-194
    • Jepsen, J.S.1    Wengel, J.2
  • 26
    • 27244447208 scopus 로고    scopus 로고
    • Locked Nucleic Acid (LNA): High Affinity Targeting of RNA for Diagnostics and Therapeutics
    • Kauppinen S., Vester B., and Wengel J. Locked Nucleic Acid (LNA): High Affinity Targeting of RNA for Diagnostics and Therapeutics. Drug Discov. Today 2 3 (2005) 287-290
    • (2005) Drug Discov. Today , vol.2 , Issue.3 , pp. 287-290
    • Kauppinen, S.1    Vester, B.2    Wengel, J.3
  • 27
    • 20644445845 scopus 로고    scopus 로고
    • Liquid Chromatography/Electrospray Mass Spectrometric Analysis of Metabolites from an Inhibitory RNA Duplex
    • Beverly M., Hartsough K., and Machemer L. Liquid Chromatography/Electrospray Mass Spectrometric Analysis of Metabolites from an Inhibitory RNA Duplex. Rapid Commun. Mass Spectrom 19 12 (2005) 1675-1682
    • (2005) Rapid Commun. Mass Spectrom , vol.19 , Issue.12 , pp. 1675-1682
    • Beverly, M.1    Hartsough, K.2    Machemer, L.3
  • 28
    • 46749099789 scopus 로고    scopus 로고
    • Metabolite Identification of Small Interfering RNA Duplex by High-Resolution Accurate Mass Spectrometry
    • Zou Y., Tiller P., Chen I.W., Beverly M., and Hochman J. Metabolite Identification of Small Interfering RNA Duplex by High-Resolution Accurate Mass Spectrometry. Rapid Commun. Mass Spectrom 22 12 (2008) 1871-1881
    • (2008) Rapid Commun. Mass Spectrom , vol.22 , Issue.12 , pp. 1871-1881
    • Zou, Y.1    Tiller, P.2    Chen, I.W.3    Beverly, M.4    Hochman, J.5
  • 29
    • 54749151857 scopus 로고    scopus 로고
    • Sequence Confirmation of Modified Oligonucleotides Using Chemical Degradation, Electrospray Ionization, Time-of-Flight, and Tandem Mass Spectrometry
    • Farand J., and Beverly M. Sequence Confirmation of Modified Oligonucleotides Using Chemical Degradation, Electrospray Ionization, Time-of-Flight, and Tandem Mass Spectrometry. Anal. Chem 80 19 (2008) 7414-7421
    • (2008) Anal. Chem , vol.80 , Issue.19 , pp. 7414-7421
    • Farand, J.1    Beverly, M.2
  • 30
    • 66149106298 scopus 로고    scopus 로고
    • De Novo Sequence Determination of Modified Oligonucleotides
    • Farand J., and Gosselin F. De Novo Sequence Determination of Modified Oligonucleotides. Anal. Chem 81 10 (2009) 3723-3730
    • (2009) Anal. Chem , vol.81 , Issue.10 , pp. 3723-3730
    • Farand, J.1    Gosselin, F.2
  • 31
    • 0001678484 scopus 로고
    • Ion-Trap Tandem Mass Spectrometry Applied to Small Multiply-Charged Oligonucleotides with a Modified Base
    • McLuckey S.A., and Habibigoudarzi S. Ion-Trap Tandem Mass Spectrometry Applied to Small Multiply-Charged Oligonucleotides with a Modified Base. J. Am. Soc. Mass Spectrom 5 8 (1994) 740-747
    • (1994) J. Am. Soc. Mass Spectrom , vol.5 , Issue.8 , pp. 740-747
    • McLuckey, S.A.1    Habibigoudarzi, S.2
  • 32
    • 23044490796 scopus 로고    scopus 로고
    • Gas-Phase Dissociation of Oligoribonucleotides and their Analogues Studied by Electrospray Ionization Tandem Mass Spectrometry
    • Tromp J.M., and Schürch S. Gas-Phase Dissociation of Oligoribonucleotides and their Analogues Studied by Electrospray Ionization Tandem Mass Spectrometry. J. Am. Soc. Mass Spectrom 16 (2005) 1262-1268
    • (2005) J. Am. Soc. Mass Spectrom , vol.16 , pp. 1262-1268
    • Tromp, J.M.1    Schürch, S.2
  • 33
    • 0035564258 scopus 로고    scopus 로고
    • Complete Sequencing of Mono-Deprotonated Peptide Nucleic Acids by Sustained Off-Resonance Irradiation Collision-Induced Dissociation
    • Flora J.W., and Muddiman D.C. Complete Sequencing of Mono-Deprotonated Peptide Nucleic Acids by Sustained Off-Resonance Irradiation Collision-Induced Dissociation. Rapid Commun. Mass Spectrom 12 7 (2001) 805-809
    • (2001) Rapid Commun. Mass Spectrom , vol.12 , Issue.7 , pp. 805-809
    • Flora, J.W.1    Muddiman, D.C.2
  • 34
    • 56449108176 scopus 로고    scopus 로고
    • Collision-Induced Dissociation of Intact Duplex and Single-Stranded siRNA Anions
    • Huang T.-Y., Liu J., Liang X., Hodges B.D.M., and McLuckey S.A. Collision-Induced Dissociation of Intact Duplex and Single-Stranded siRNA Anions. Anal. Chem 80 (2008) 8501-8508
    • (2008) Anal. Chem , vol.80 , pp. 8501-8508
    • Huang, T.-Y.1    Liu, J.2    Liang, X.3    Hodges, B.D.M.4    McLuckey, S.A.5
  • 35
    • 0029194414 scopus 로고
    • Utility of Organic Bases for Improved Electrospray Mass Spectrometry of Oligonucleotides
    • Greig M., and Griffey R.M. Utility of Organic Bases for Improved Electrospray Mass Spectrometry of Oligonucleotides. Rapid Commun. Mass Spectrom 9 (1995) 97-102
    • (1995) Rapid Commun. Mass Spectrom , vol.9 , pp. 97-102
    • Greig, M.1    Griffey, R.M.2
  • 37
    • 27144458820 scopus 로고    scopus 로고
    • Pulsed Duel Electrospray Ionization for Ion/Ion Reactions
    • Xia Y., Liang X., and McLuckey S.A. Pulsed Duel Electrospray Ionization for Ion/Ion Reactions. J. Am. Soc. Mass Spectrom 16 (2005) 1750-1756
    • (2005) J. Am. Soc. Mass Spectrom , vol.16 , pp. 1750-1756
    • Xia, Y.1    Liang, X.2    McLuckey, S.A.3
  • 38
    • 2442496406 scopus 로고    scopus 로고
    • Sequence-Dependent Thermodynamic Parameters for Locked Nucleic Acid (LNA)-DNA Duplex Formation
    • McTigue P.M., Peterson R.J., and Kahn J.D. Sequence-Dependent Thermodynamic Parameters for Locked Nucleic Acid (LNA)-DNA Duplex Formation. Biochemistry 43 18 (2004) 5388-5405
    • (2004) Biochemistry , vol.43 , Issue.18 , pp. 5388-5405
    • McTigue, P.M.1    Peterson, R.J.2    Kahn, J.D.3
  • 39
    • 44049123270 scopus 로고
    • Mass Spectrometry/Mass Spectrometry of Small Multiply Charged Oligonucleotides
    • McLuckey S.A., Van Berkel G.J., and Glish G.L. Mass Spectrometry/Mass Spectrometry of Small Multiply Charged Oligonucleotides. J. Am. Soc. Mass Spectrom 3 (1992) 60-70
    • (1992) J. Am. Soc. Mass Spectrom , vol.3 , pp. 60-70
    • McLuckey, S.A.1    Van Berkel, G.J.2    Glish, G.L.3
  • 40
    • 5344270491 scopus 로고    scopus 로고
    • Gas-Phase Fragmentation of Oligonucleotide Ions
    • Wu J., and McLuckey S.A. Gas-Phase Fragmentation of Oligonucleotide Ions. Int. J. Mass Spectrom 237 (2004) 197-241
    • (2004) Int. J. Mass Spectrom , vol.237 , pp. 197-241
    • Wu, J.1    McLuckey, S.A.2
  • 41
    • 0036676658 scopus 로고    scopus 로고
    • Electrospray Tandem Mass Spectrometry of Mixed-Sequence RNA/DNA Oligonucleotides
    • Schürch S., Bernal-Mendez E., and Leumann C.J. Electrospray Tandem Mass Spectrometry of Mixed-Sequence RNA/DNA Oligonucleotides. J. Am. Soc. Mass Spectrom 13 (2002) 936-945
    • (2002) J. Am. Soc. Mass Spectrom , vol.13 , pp. 936-945
    • Schürch, S.1    Bernal-Mendez, E.2    Leumann, C.J.3
  • 42
    • 33748984285 scopus 로고    scopus 로고
    • RNA Fragmentation in MALDI Mass Spectrometry Studied by H/D-Exchange: Mechanisms of General Applicability to Nucleic Acids
    • Andersen T.E., Kirpekar F., and Haselmann K.F. RNA Fragmentation in MALDI Mass Spectrometry Studied by H/D-Exchange: Mechanisms of General Applicability to Nucleic Acids. J. Am. Soc. Mass Spectrom 17 (2006) 1353-1368
    • (2006) J. Am. Soc. Mass Spectrom , vol.17 , pp. 1353-1368
    • Andersen, T.E.1    Kirpekar, F.2    Haselmann, K.F.3
  • 43
    • 0000529864 scopus 로고
    • Decompositions of Multiply-Charged Oligonucleotide Anions
    • McLuckey S.A., and Habibi-Goudarzi S. Decompositions of Multiply-Charged Oligonucleotide Anions. J. Am. Chem. Soc 115 (1993) 12085-12095
    • (1993) J. Am. Chem. Soc , vol.115 , pp. 12085-12095
    • McLuckey, S.A.1    Habibi-Goudarzi, S.2
  • 44
    • 0029164899 scopus 로고
    • Charged vs. Neutral Nucleobase Loss from Multiply Charged Oligonucleotide Anions
    • McLuckey S.A., Vaidyanathan G., and Habibi-Goudarzi S. Charged vs. Neutral Nucleobase Loss from Multiply Charged Oligonucleotide Anions. J. Mass Spectrom 30 (1995) 1222-1229
    • (1995) J. Mass Spectrom , vol.30 , pp. 1222-1229
    • McLuckey, S.A.1    Vaidyanathan, G.2    Habibi-Goudarzi, S.3
  • 45
    • 56949103381 scopus 로고    scopus 로고
    • Ion Trap Collision-Induced Dissociation of Multiply Deprotonated RNA: c/y-Ions Versus (a-B)/w-Ions
    • Huang T.-Y., Kharlamova A., Liu J., and McLuckey S.A. Ion Trap Collision-Induced Dissociation of Multiply Deprotonated RNA: c/y-Ions Versus (a-B)/w-Ions. J. Am. Soc. Mass Spectrom 19 12 (2008) 1832-1840
    • (2008) J. Am. Soc. Mass Spectrom , vol.19 , Issue.12 , pp. 1832-1840
    • Huang, T.-Y.1    Kharlamova, A.2    Liu, J.3    McLuckey, S.A.4
  • 46
    • 0032232419 scopus 로고    scopus 로고
    • Structure and Fragmentation Mechanisms of Isomeric T-Rich Oligodeoxynucleotides: A Comparison of Four Tandem Mass Spectrometric Methods
    • Wang Z., Wan K.X., Ramanathan R., Taylor J.S., and Gross M.L. Structure and Fragmentation Mechanisms of Isomeric T-Rich Oligodeoxynucleotides: A Comparison of Four Tandem Mass Spectrometric Methods. J. Am. Soc. Mass Spectrom 9 (1998) 683-691
    • (1998) J. Am. Soc. Mass Spectrom , vol.9 , pp. 683-691
    • Wang, Z.1    Wan, K.X.2    Ramanathan, R.3    Taylor, J.S.4    Gross, M.L.5
  • 47
    • 0035561180 scopus 로고    scopus 로고
    • Fragmentation Mechanisms of Oligodeoxynucleotides Studied by H/D Exchange and Electrospray Ionization Tandem Mass Spectrometry
    • Wan K.X., Gross J., Hillenkamp F., and Gross M.L. Fragmentation Mechanisms of Oligodeoxynucleotides Studied by H/D Exchange and Electrospray Ionization Tandem Mass Spectrometry. J. Am. Soc. Mass Spectrom 12 (2001) 193-205
    • (2001) J. Am. Soc. Mass Spectrom , vol.12 , pp. 193-205
    • Wan, K.X.1    Gross, J.2    Hillenkamp, F.3    Gross, M.L.4
  • 48
    • 0035567094 scopus 로고    scopus 로고
    • Fragmentation Mechanisms of Oligodeoxynucleotides: Effects of Replacing Phosphates with Methylphosphonates and Thymines with Other Bases in T-Rich Sequences
    • Wan K.X., and Gross M.L. Fragmentation Mechanisms of Oligodeoxynucleotides: Effects of Replacing Phosphates with Methylphosphonates and Thymines with Other Bases in T-Rich Sequences. J. Am. Soc. Mass Spectrom 12 (2001) 580-589
    • (2001) J. Am. Soc. Mass Spectrom , vol.12 , pp. 580-589
    • Wan, K.X.1    Gross, M.L.2


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