Design and characterization of decoy oligonucleotides containing locked nucleic acids

Nucleic Acids Research

Volumn 30, Issue 11, 2002, Pages 2435-2443

  Crinelli, Rita ^a   Bianchi, Marzia ^a   Gentilini, Lucia ^a   Magnani, Mauro ^a  

^a UNIVERSITY OF URBINO   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

COPOLYMER; DNA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; NUCLEASE; NUCLEIC ACID; OLIGONUCLEOTIDE;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL STRAIN 3T3; CONSENSUS SEQUENCE; CONTROLLED STUDY; ENZYME DEGRADATION; GENE THERAPY; GENETIC STABILITY; GENETIC TRANSFECTION; IN VIVO STUDY; MOUSE; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; PROTEIN BINDING;

ANIMALIA; INSERTION SEQUENCES;

EID: 0036606669     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/30.11.2435     Document Type: Article

References (49)

1. Transcription-factor-modulating agents: Precision and selectivity in drug design
2. Application of transcription factor "decoy" strategy as means of gene therapy and study of gene expression in cardiovascular disease
3. Oligonucleotides as transcription factor decoys
4. Therapeutic applications of transcription factor decoy oligonucleotides
5. Regulation of gene expression with double-stranded phosphorothioate oligonucleotides
6. A gene therapy strategy using a transcription factor decoy of the E2F binding site inhibits smooth muscle proliferation in vivo
7. In vivo transfection of cis element "decoy" against nuclear factor -kB binding site prevents myocardial infarction
8. Antisense oligonucleotides: A new therapeutic principle
9. The stability of different forms of double-stranded decoy DNA in serum and nuclear extracts
10. Physicochemical properties of phosphorothioate oligodeoxynucleotides
11. Comparison of cellular binding and uptake of antisense phosphodiester, phosphorothioate and mixed phosphorothioate and methylphosphonate oligonucleotides
12. Duplex stabilities of phosphorothioate, methylphosphonate and RNA analogs of two DNA 14-mers
13. Phosphorothioate oligonucleotides are inhibitors of human DNA polymerases and RNase H: Implications for antisense technology
14. Effects of sequence of thioated oligonucleotides on cultured human mammary epithelial cells
15. Effect of phosphorothioate modification of oligodeoxynucleotides on specific protein binding
16. Interaction of the human NF-kB p52 transcription factor with DNA-PNA hybrids mimicking the NF-kB binding sites of the human immunodeficiency virus type 1 promoter
17. PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules
18. Stability of peptide nucleic acids in human serum and cellular extracts
19. LNA (locked nucleic acids): Synthesis and high-affinity nucleic acid recognition
20. LNA (locked nucleic acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation and unprecedented nucleic acid recognition
21. Synthesis of 3′-C-and 4′-C-branched oligodeoxynucleotides and the development of locked nucleic acid (LNA)
22. Universality of LNA-mediated high-affinity nucleic acid recognition
23. Stability and structural features of the duplexes containing nucleoside analogues with a fixed N-type conformation, 2′-O,4′-C-methyleneribonucleosides
24. Oligonucleotides containing 4′-C-aminomethyl-2′-modified thymidines show increased binding affinity towards DNA and RNA
25. Stopped-flow kinetics of locked nucleic acid (LNA)-oligonucleotide duplex formation: Studies of LNA-DNA and DNA-DNA interactions
26. Potent and nontoxic antisense oligonucleotides containing locked nucleic acids
27. The Rel/NF-kB family: Friend and foe
28. Series introduction: The transcription factor NF-kB and human diseases
29. Transcription factor decoy approach to decifer the role of NF-kB in oncogenesis
30. NF-kappaB activation provides the potential link between inflammation and hyperplasia in the arthritic joint
31. Hypoxia-induced endothelial apoptosis through nuclear factor-kappaB (NF-kappaB)-mediated bcl-2 suppression: In vivo evidence of the importance of NF-kappaB in endothelial cell regulation
32. Attenuation of interleukin-8 production by inhibiting nuclear factor-kappaB translocation using decoy oligonucleotides
33. Transcription factor decoy for NFkappaB inhibits TNF-alpha-induced cytokine and adhesion molecule expression in vivo
34. The solution structure of a locked nucleic acid (LNA) hybridized to DNA
35. Solution structure of an LNA hybridized to DNA: NMR study of the d(CT(L)GCT(L)T(L)CT(L)GC):d(GCAGAAGCAG) duplex containing four locked nucleotides
36. The conformations of locked nucleic acids (LNA)
37. Structure of NF-kappa B p50 homodimer bound to a kB site
38. Structure of the NF-kappa B p50 homodimer bound to DNA
39. Crystal structure of p50/p65 heterodimer of transcription factor NF-kappaB bound to DNA
40. The high mobility group protein HMG I(Y) is required for NF-kappa B-dependent virus induction of the human IFN-beta gene
41. NF-κB mediated transcriptional activation is enhanced by the architectural factor HMGI-C
42. Antisense oligonucleotides as therapeutic agents. Is the bullet really magical?
43. Modified deoxyoligonucleotides stable to exonuclease degradation in serum
44. Studies on the mechanism of stabilization of partially phosphorothioated oligonucleotides against nucleolytic degradation
45. Evaluation of different types of end-capping modifications on the stability of oligonucleotides towards 3′- and 5′-exonucleases
46. Structure of Dnase I at 2.0 Å resolution suggests a mechanism for binding to and cutting DNA
47. DNA recognition by structure-selective nucleases
48. Molecular interactions between nuclear factor-kB (NF-kB) transcription factors and PNA-DNA chimera mimicking NF-kB binding sites
49. Locked nucleic acids (LNA): Fine-tuning the recognition of DNA and RNA