Direct detection of abortive RNA transcripts in vivo

Science

Volumn 324, Issue 5929, 2009, Pages 927-928

  Goldman, Seth R ^a   Ebright, Richard H ^a   Nickels, Bryce E ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ELONGATION FACTOR; ELONGATION FACTOR GREA; RNA; RNA POLYMERASE; SIGMA FACTOR; UNCLASSIFIED DRUG; BACTERIAL RNA; DNA DIRECTED RNA POLYMERASE; ESCHERICHIA COLI PROTEIN; GREA PROTEIN, E COLI; TRANSCRIPTION FACTOR;

DETECTION METHOD; HYBRIDIZATION; POLYMERASE CHAIN REACTION; RNA;

ARTICLE; BACTERIUM; GENE EXPRESSION; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; REGULATORY MECHANISM; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; NUCLEIC ACID HYBRIDIZATION; NUCLEIC ACID PROBE; PLASMID; PROMOTER REGION;

EUKARYOTA; PROKARYOTA;

DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; NUCLEIC ACID HYBRIDIZATION; NUCLEIC ACID PROBES; PLASMIDS; PROMOTER REGIONS, GENETIC; RNA, BACTERIAL; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

EID: 66149123741     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1169237     Document Type: Article

References (20)

1. L. M. Hsu, Biochim. Biophys. Acta 1577, 191 (2002).
2. K. S. Murakami, S. A. Darst, Curr. Opin. Struct. Biol. 13, 31 (2003).
3. L. M. Hsu, Methods 47, 25 (2009).
4. A. N. Kapanidis et al., Science 314, 1144 (2006).
5. E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, S. M. Block, Nature 438, 460 (2005).
6. W. R. McClure, C. L. Cech, J. Biol. Chem. 253, 8949 (1978).
7. A. J. Carpousis, J. D. Gralla, Biochemistry 19, 3245 (1980).
8. J. D. Gralla, A. J. Carpousis, J. E. Stefano, Biochemistry 19, 5864 (1980).
9. L. M. Hsu, N. V. Vo, M. J. Chamberlin, Proc. Natl. Acad. Sci. U.S.A. 92, 11588 (1995).
10. M. Monsalve, M. Mencia, F. Rojo, M. Salas, EMBO J. 15, 383 (1996).
11. C. L. Chan, C. A. Gross, J. Biol. Chem. 276, 38201 (2001).
12. L. M. Hsu, N. V. Vo, C. M. Kane, M. J. Chamberlin, Biochemistry 42, 3777 (2003).
13. N. V. Vo, L. M. Hsu, C. M. Kane, M. J. Chamberlin, Biochemistry 42, 3798 (2003).
14. W. Kammerer, U. Deuschle, R. Gentz, H. Bujard, EMBO J. 5, 2995 (1986).
15. Materials and methods are available as supporting material on Science Online.
16. A. Valoczi et al., Nucleic Acids Res. 32, e175 (2004).
17. T. Ellinger, D. Behnke, H. Bujard, J. D. Gralla, J. Mol. Biol. 239, 455 (1994).
18. D. C. Ko, M. T. Marr, J. Guo, J. W. Roberts, Genes Dev. 12, 3276 (1998).
19. M. Leibman, A. Hochschild, EMBO J. 26, 1579 (2007).
20. We thank A. Hochschild, A. Revyakin, and J. Roberts for materials, and A. Gann, S. Garrity, A. Hochschild, L. Hsu, W. McAllister, E. McGlinn, and R. Sousa for discussion. Work was supported by NIH grant GM41376 and a Howard Hughes Medical Institute Investigatorship to R.H.E. and by a Pew Scholars Award to B.E.N.