Primary DNA damage assessed with the comet assay and comparison to the absorbed dose of diagnostic X-rays in children

International Journal of Toxicology

Volumn 28, Issue 5, 2009, Pages 405-416

  Milković, Durdica ^a   Garaj Vrhovac, Vera ^b   Ranogajec Komor, Mária ^c   Miljanić, Saveta ^c   Gajski, Goran ^b   Knežević, Željka ^c   Beck, Natko ^a  

^a SREBRNJAK CHILDREN S HOSPITAL   (Croatia)

^b INSTITUTE FOR MEDICAL RESEARCH AND OCCUPATIONAL HEALTH   (Croatia)

^c RUDER BO KOVI INSTITUTE   (Croatia)

Author keywords

ALARA; Alkaline comet assay; DNA damage; Human peripheral blood lymphocytes; X ray dosimetry

Indexed keywords

ADOLESCENT; ARTICLE; CHILD; CLINICAL ARTICLE; COMET ASSAY; CORRELATION ANALYSIS; DNA DAMAGE; FEMALE; HUMAN; HUMAN CELL; MALE; PERIPHERAL LYMPHOCYTE; PHOTOLUMINESCENCE; PRESCHOOL CHILD; RADIATION ABSORPTION; RADIATION DOSE; RADIATION INJURY; SCHOOL CHILD; THERMOLUMINESCENCE DOSIMETER; THORAX RADIOGRAPHY;

ADOLESCENT; CHILD; CHILD, PRESCHOOL; COMET ASSAY; DNA DAMAGE; DNA REPAIR; FEMALE; FOREHEAD; GAMMA RAYS; GONADS; HUMANS; LUNG DISEASES; LYMPHOCYTES; MALE; RADIATION DOSAGE; RADIOMETRY; THYROID GLAND;

EID: 73149103954     PISSN: 10915818     EISSN: 1092874X     Source Type: Journal    
DOI: 10.1177/1091581809344775     Document Type: Article

References (72)

1. Sankaranarayanan K. Estimation of the hereditary risks of exposure to ionizing radiation: history, current status, and emerging perspectives. Health Phys. 2001;80: 363-369.
2. Sankaranarayanan K. Estimation of the genetic risks of exposure to ionizing radiation in humans: current status and emerging perspectives. J Radiat Res (Tokyo). 2006; 47(suppl B):B57-66.
3. Schneider K, Kohn MM, Ernst G. The derivation of reference dose values to chest x-rays in paediatric radiography. Radiat Prot Dosim. 1998;80:199-202.
4. Cook JV, Kyriou JC, Pettet A, et al. Key factors in the optimization of paediatric X-ray practice. Br J Radiol. 2001;74:1032-1040.
5. Simpson AK, Whang PG, Jonisch A, Haims A, Grauer JN. The radiation exposure associated with cervical and lumbar spine radiographs. J Spinal Disord Tech. 2008;21:409-412.
6. Alt CD, Engelmann D, Schenk JP, Troeger J. Quality control of thoracic X-rays in children in diagnostic centers with and without pediatric-radiologic competence. Rofo. 2006;178:191-199.
7. Seidenbusch MC, Schneider K. radiation exposure of children in pediatric radiology. Rofo. 2008;180:410-422.
8. Seidenbusch MC, Regulla D, Schneider K. Radiation exposure of children in pediatric radiology, part 2: the PAEDOS algorithm for computer-assisted dose reconstruction in pediatric radiology and results for X-ray examinations of the skull. Rofo. 2008;180:522-539.
9. Seidenbusch MC, Regulla D, Schneider K. Radiation exposure of children in pediatric radiology, part 3: Conversion coefficients for reconstruction of organ doses achieved during chest X-ray examinations. Rofo. 2008; 180:1061-1081.
10. Seidenbusch MC, Schneider K. Radiation exposure of children in pediatric radiology, part 4: Entrance doses achieved during the X-ray examination of the chest. Rofo. 2008;180:1082-1103.
11. Seidenbusch MC, Schneider K. Radiation exposure of children in pediatric radiology, part 5: organ doses in chest radiography. Rofo. 2009;181:454-471.
12. Hammer GP, Seidenbusch MC, Schneider K, et al. A cohort study of childhood cancer incidence after postnatal diagnostic X-ray exposure. Radiat Res. 2009; 171:504-512.
13. Milković D, Ranogajec-Komor M, Krstić-Burić M, Hebrang A. Mit Thermolumineszenz-Dosimetern gemessene Hautdosen bei Thorax-Rontgenaufnahmen von Kindern und Jugendlichen. Atemw Lungenkrkh. 2001; 17:B67-B72.
14. Strauss KJ, Kaste SC. ALARA in pediatric interventional and fluoroscopic imaging: striving to keep radiation doses as low as possible during fluoroscopy of pediatric patients: a white paper executive summary. J Am Coll Radiol. 2006;3:686-688.
15. Strauss KJ, Kaste SC. The ALARA (as low as reasonably achievable) concept in pediatric interventional and fluoroscopic imaging: striving to keep radiation doses as low as possible during fluoroscopy of pediatric patients: a white paper executive summary. Radiology. 2006;240:621-622.
16. Tice RR, Strauss GH. The single cell gel electrophoresis/comet assay: a potential tool for detecting radiationinduced DNA damage in humans. Stem Cells. 1995; 13:207-214.
17. Garaj-Vrhovac V, Kopjar N, Razem D, et al. Application of the alkaline comet assay in biodosimetry: assessment of in vivo DNA damage in human peripheral leukocytes after a gamma radiation incident. Radiat Prot Dosimetry. 2002;98:407-416.
18. Garaj-Vrhovac V, Kopjar N. The alkaline Comet assay as biomarker in assessment of DNA damage in medical personnel occupationally exposed to ionizing radiation. Mutagenesis. 2003;18:265-271.
19. Dusinská M, Collins AR. The comet assay in human biomonitoring: gene-environment interactions. Mutagenesis. 2008;23:191-205.
20. Collins AR. Comet assay for DNA damage and repair: principles, applications and limitations. Mol Biotechnol. 2004;26:249-261.
21. Collins AR, Oscoz AA, Brunborg G, et al. The comet assay: topical issues. Mutagenesis. 2008;23:143-151.
22. Gajski G, Garaj-Vrhovac V, Orescanin V. Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: Standard and Fpg-modified comet assay. Toxicol Appl Pharmacol. 2008;231:85-93.
23. McKenna DJ, McKeown SR, McKelvey-Martin VJ. Potential use of the comet assay in the clinical management of cancer. Mutagenesis. 2008;23:183-190.
24. Collins AR,Dusinská M, Franklin M, et al. Comet Assay in human biomonitoring studies: reliability, validation, and applications. Environ Mol Mutagen. 1997;30:139-146.
25. Garaj-Vrhovac V, Kopjar N. Investigation into possible DNA damaging effects of ultrasound in occupationally exposed medical personnel: the alkaline comet assay study. J Appl Toxicol. 2005;25:184-192.
26. Kumaravel TS, Vilhar B, Faux SP, Jha AN. Comet assay measurements: a perspective. Cell Biol Toxicol. 2009; 25:53-64.
27. Hagmar L, Strömberg U, Tinnerberg H, Mikoczy Z. The usefulness of cytogenetic biomarkers as intermediate endpoints in carcinogenesis. Int J Hyg Environ Health. 2001;204:43-47.
28. Ahuja YR, Rajeshwari N. What is my risk of cancer? Int J Hum Gene. 2003;3:109-113.
29. Kyriou JC, Fitzgerald M, Pettett A, Cook JV, Pablot SM. A comparison of doses and techniques between specialist and non-specialist centres in the diagnostic X-ray imaging of children. Br J Radiol. 1996;69:437-450.
30. United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR). Sources and Effects of Ionizing Radiation. UNSCEAR 2000 Report II: Effects. Vienna: UNSCEAR; 2000.
31. Lavin MF, Shiloh Y. Ataxia-telangiectasia: a multifaceted genetic disorder associated with defective signal transduction. Curr Opin Immunol. 1996;8:459-464.
32. Milković D, Ranogajec-Komor M, Krstić-Burić M, Hebrang A. Bestimmung der Bestrahlungsdosis in radiologischer Diagnostik paranasaler Sinusse bei Kindern und Jugendlichen. Atemw Lungenkrkh. 1993;19: 101-104.
33. Milković D, Ranogajec-Komor M, Ivanovi-Herceg Z, et al. Doses in the vicinity of mobile X-ray equipment in a children's intensive care unit. Coll Antropol. 2000;24: 235-240.
34. Ranogajec-Komor M, Muhiy-Ed-Din F, Milković D, Vekić B. Thermoluminescence characteristics of various detectors for X-ray diagnostic measurements. Radiat Prot Dosim. 1993;47:529-534.
35. Ranogajec-Komor M, Nikodemová D, Horváthová M, Knežević Ž, Milković D. Radiation exsposure of children during radiognostic examination of chest. In: Sabol J, ed. Proceedings of the IRPA Regional Symposium: Radiation Protection in Neighbouring Countries of Central Europe, Prague. Prague: Czech Technical University; 1997: 298-300.
36. Hsu SM, Yeh SH, Lin MS, Chen WL. Comparison on characteristics of radiophotoluminescent glass dosemeters and thermoluminescent dosemeters. Radiat Prot Dosimetry. 2006;19:327-331.
37. Milković D, Ranogajec-Komor M, Miljanić S, Knežević Ž, Krpan K. Comparison of different dosimetry system for dose measurements in diagnostic radiology. In: Proceedings of Second European IRPA Congress 2006. Paris, France: Société Françarse de Radioprotection; 2006: P-091:1-10.
38. Ranogajec-Komor M, Knežević Ž, Miljanić S, Vekić B. Characterisation of radiophotoluminescent dosimeters for environmentalmonitoring.RadiatMeas. 2008;43:392-396.
39. Miljanić S, Ranogajec-Komor M, Knežević Ž, Vekić B. Main dosimetric characteristics of some "tissue- equivalent" TL detectors. Radiat Prot Dosim. 2002;100:437-442.
40. Vekić B, Ban R, Miljanić S. Secondary standard dosimetry laboratory at the Ruder Bošković Insititute. Arh Hig Rada Toksikol. 2006;57:189-194.
41. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988;175:184-191.
42. Betti C, Davini T, Giannessi L, Loprieno N, Barale R. Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects. Mutat Res. 1994;307:323-333.
43. Brenner DJ, Doll R, Goodhead DT, et al. Cancer risks attributable to low doses of ionizing radiation: assessing what we really know. Proc Natl Acad Sci U S A. 2003;100:13761-13766.
44. Berrington de González A, Darby S. Risk of cancer from diagnostic X-rays: estimates for the UK and 14 other countries. Lancet. 2004;363:345-351.
45. Brenner DJ, Hall EJ. Risk of cancer from diagnostic X-rays. Lancet. 2004;363:2192-2193.
46. Hall EJ, Brenner DJ. Cancer risks from diagnostic radiology. Br J Radiol. 2008;81:362-378.
47. Linet MS, Kim KP, Rajaraman P. Children's exposure to diagnostic medical radiation and cancer risk: epidemiologic and dosimetric considerations. Pediatr Radiol. 2009;39:S4-26.
48. Ron E. Cancer risks from medical radiation. Health Phys. 2003;85:47-59.
49. Fucic A, Brunborg G, Lasan R, et al. Genomic damage in children accidentally exposed to ionizing radiation: a review of the literature. Mutat Res. 2008;658:111-123.
50. Preston DL, Cullings H, Suyama A, et al. Solid cancer incidence in atomic bomb survivors exposed in utero or as young children. J Natl Cancer Inst. 2008;100:428-436.
51. Wakeford R. Childhood leukaemia following medical diagnostic exposure to ionizing radiation in utero or after birth. Radiat Prot Dosimetry. 2008;132:166-174.
52. Shore RE, Hildreth N, Dvoretsky P. Thyroid cancer among persons given X-ray treatment in infancy for an enlarged thymus gland. Am J Epidemiol. 1993;137: 1068-1080.
53. Shore RE, Moseson M, Xue X. Skin cancer after X-ray treatment for scalp ringworm. Radiat Res. 2002;157: 410-418.
54. Gilbert ES. Ionising radiation and cancer risks: What have we learned from epidemiology? Int J Radiat Biol. 2009 [Epub ahead of print].
55. Kleinerman RA. Cancer risks following diagnostic and therapeutic radiation exposure in children. Pediatr Radiol. 2006;36:121-125.
56. Tice RR, Agurell E, Anderson D, et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000;35: 206-221.
57. Kruszewski M, Wojewódzka M, Iwanenko T, Collins AR, Szumiel I. Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation, II: base damage. Mutat Res. 1998;416:37-57. (Pubitemid 28398845)
58. Wojewódzka M, Kruszewski M, Iwanenko T, Collins AR, Szumiel I. Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation, I: strand breakage. Mutat Res. 1998;416: 21-35. (Pubitemid 28398844)
59. Olive PL. DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int J Rad Biol. 1999;75:395-405.
60. Gajski G, Garaj-Vrhovac V. Genotoxic potential of bee venom (Apis mellifera) on human peripheral blood lymphocytes in vitro using single cell gel electrophoresis assay. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2008;43:1279-1287.
61. Garaj-Vrhovac V, Gajski G, Trošić I, Pavičić I. Evaluation of basal DNA damage and oxidative stress in Wistar rat leukocytes after exposure tomicrowave radiation. Toxicology. 2009;259:107-112.
62. Piperakis SM, Kontogianni K, Karanastasi G, Iakovidou- Kritsi Z, PiperakisMM. The use of comet assay inmeasuring DNA damage and repair efficiency in child, adult, and old age populations. Cell Biol Toxicol. 2009;25:65-71.
63. MohrenweiserHW, Jones IM. Variation inDNA repair is a factor in cancer susceptibility: a paradigm for the promises and perils of individual and population risk estimation? Mutat Res. 1998;400:15-24.
64. Sasaki YF, Sekihashi K, Izumiyama F, et al. The comet assay with multiple mouse organs: comparison of comet assay results and carcinogenicity with 208 chemicals selected from the IARCmonographs and U.S.NTP Carcinogenicity Database. Crit Rev Toxicol. 2000;30:629-799.
65. Wang AS, Ramanathan B, Chien YH, Goparaju CM, Jan KY. Comet assay with nuclear extract incubation. Anal Biochem. 2005;337:70-75.
66. Hagmar L, Bonassi S, Strömberg U, et al. Cancer predictive value of cytogenetic markers used in occupational health surveillance programs. Recent Results Cancer Res. 1998;154:177-184.
67. Hagmar L, Bonassi S, Strömberg U, et al. Cancer predictive value of cytogenetic markers used in occupational health surveillance programs: a report from an ongoing study by the European Study Group on Cytogenetic Biomakers and Health. Mutat Res. 1998b;405:171-178.
68. Kumaravel TS, Bristow RG. Detection of genetic instability at HER-2/neu and p53 loci in breast cancer cells sing Comet-FISH. Breast Cancer Res Treat. 2005;91:89-93.
69. Waters DJ, Shen S, Xu H, et al. Noninvasive prediction of prostatic DNA damage by oxidative stress challenge of peripheral blood lymphocytes. Cancer Epidemiol Biomarkers Prev. 2007;16:1906-1910.
70. Kopjar N, Zeljezić D, Garaj-Vrhovac V. Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test. Acta Biochim Pol. 2006; 53:321-336.
71. He J, Chen W, Jin L, Jin H. Comet assay and cytokinesisblocked micronucleus test for monitoring the genotoxic effects of X-ray radiation in humans. Chin Med J. 2000;113:911-914.
72. Mitchel RE. Cancer and low dose responses in vivo: implications for radiation protection. Dose Response. 2007;5:284-291.