Genotoxic potential of bee venom (Apis Mellifera) on human peripheral blood lymphocytes in vitro using single cell gel electrophoresis assay

Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering

Volumn 43, Issue 11, 2008, Pages 1279-1287

  Gajski, Goran ^a   Garaj Vrhovac, Vera ^a  

^a INSTITUTE FOR MEDICAL RESEARCH AND OCCUPATIONAL HEALTH   (Croatia)

Author keywords

Bee venom; Genotoxicity; Peripheral blood human lymphocytes; Single cell gel electrophoresis assay

Indexed keywords

ABERRATIONS; ANIMAL CELL CULTURE; BLOOD; CELL DEATH; COLLOIDS; CONCENTRATION (PROCESS); DNA; ELECTROCHEMISTRY; ELECTROPHORESIS; GELATION; GELS; GENES; HEALTH; MILITARY CONSTRUCTION EQUIPMENT; NUCLEIC ACIDS; OPTICAL PROPERTIES; ORGANIC ACIDS;

ANTI-INFLAMMATORY; ANTICANCER EFFECTS; APIS MELLIFERA; APOPTOSIS; BEE VENOM; CANCER CELLS; CONCENTRATION (COMPOSITION); CONTROL CELLS; DNA DAMAGES; DOSE DEPENDENT; GENOTOXIC POTENTIAL; GENOTOXICITY; HUMAN CELLS; HUMAN LYMPHOCYTES; HUMAN PERIPHERAL BLOOD; IN-VITRO; PERIPHERAL BLOOD HUMAN LYMPHOCYTES; SINGLE CELL GEL ELECTROPHORESIS (SCGE); TAIL LENGTH; TAIL MOMENT (TM); TIME PERIODS;

CELLS;

BEE VENOM;

ADULT; ARTICLE; CELL VIABILITY; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; GENOTOXICITY; HONEYBEE; HUMAN; HUMAN CELL; IN VITRO STUDY; MALE; PERIPHERAL LYMPHOCYTE;

ANIMALS; BEE VENOMS; BEES; COMET ASSAY; HUMANS; LYMPHOCYTES; MUTAGENS;

APIS MELLIFERA; APOIDEA;

EID: 47649103928     PISSN: 10934529     EISSN: 15324117     Source Type: Journal    
DOI: 10.1080/10934520802177862     Document Type: Article

References (37)

1. Billingham, M.E.; Morley, J.; Hanson, J.M.; Shipolini, R.A.; Vernon, C.A. Letter: An anti-inflammatory peptide from bee venom. Nature 2001, 245(5421), 163-164.
2. Hider, R.C. Honeybee venom: a rich source of pharmacologically active peptides. Endeavour 1988, 12(2), 60-65.
3. Varanda, E.A.; Tavares, D.C. Radioprotection: Mechanism and radioprotective agents including honey bee venom. Venom. Anim. Toxins 1998, 4(1), 5-21.
4. Varanda, E.A.; Monti R.; Tavares, D.C. Inhibitory effect of propolis and bee venom on the mutagenicity of some direct- and indirect-acting mutagens. Teratog. Carcinog. Mutagen. 1999, 19(6), 403-413.
5. Sumikura, H.; Andersen, O.K.; Drewes, A.M.; Arendt-Nielsen, L.A. Comparison of hyperalgesia and neurogenic inflammation induced by melittin and capsaicin in humans. Neurosci. Lett. 2003, 337(3), 147-150.
6. Nam, K.W.; Je, K.H.; Lee, J.H.; Han, H.J.; Lee, H.J., Kang, S.K.; Mar, W. Inhibition of COX-2 activity and proinflammatory cytokines (TNF-alpha and IL-1beta) production bywater-soluble subfractionated parts from bee (Apis mellifera) venom. Arch. Pharm. Res. 2003, 26(5), 383-388.
7. Kim, H.W.; Kwon, Y.B.; Ham, T.W.; Roh, D.H.; Yoon, S.Y.; Lee, H.J.; Han, H.J.; Yang, I.S.; Beitz, A.J.; Lee, J.H. Acupoint stimulation using bee venom attenuates formalin-induced pain behavior and spinal cord fos expression in rats. J. Vet. Med. Sci. 2003, 65(3), 349-355.
8. Orsolic, N.; Sver, L.; Verstovsek, S.; Terzic, S.; Basic, I. Inhibition of mammary carcinoma cell proliferation in vitro and tumor growth in vivo by bee venom. Toxicon 2003, 41(7), 861-870.
9. Habermann, E. Bee and wasp venoms. Science 1972, 177(46), 314-322.
10. Dotimas, E.M.; Hamid, K.R.; Hider, R.C.; Ragnarsson, U. Isolation and structure analysis of bee venom mast cell degranulating peptide. Biochim. Biophys. Acta 1987, 911(3), 285-293.
11. Lariviere, W.R.; Melzack, R. The bee venom test: a new tonic-pain test. Pain 1996, 66(2-3), 271-277.
12. Neuman, W.; Habermann, E. Characterization of the substances of the bee venom. Naunyn Schmiedebergs Arch. Exp. Pathol. Pharmakol. 1954, 222(4), 267-287.
13. Vogel, H. Incorporation of melittin into phosphatidylcholine bilayers. Study of binding and conformational changes. FEBS Lett. 1981, 134(1), 37-42.
14. Gevod, V.S.; Birdi, K.S. Melittin and the 8-26 fragment. Differences in ionophoric properties as measured by monolayer method. Biophys. J. 1984, 45(6), 1079-1083.
15. Habermann, E. Chemistry, pharmacology, and toxicology of bee, wasp and hornet venoms. In: W. Bucherl and E.E. Buckley, Editors, Venomous Animals and Their Venoms, vol. 3. Academic Press, New York, 1971; 61-93.
16. Fletcher, J.E.; Jiang, M.S. Possible mechanisms of action of cobra snake venom cardiotoxins and bee venom melittin. Toxicon 1993, 31, 669-695.
17. Liu, X.; Chen, D.; Xie, L.; Zhang, R. Effect of honey bee venom on proliferation of K1735M2 mouse melanoma cells in-vitro and growth of murine B16 melanomas in-vivo. J. Pharm. Pharmacol. 2002, 54(8), 1083-1089.
18. Jang, M.H.; Shin, M.C.; Lim, S.; Han, S.M.; Park, H.J.; Shin, I. Bee venom induces apoptosis and inhibits expression of cyclooxygenase-2 mRNA in human lung cancer cell line NCI-H1299. J. Pharmacol. Sci. 2003, 91(2), 95-104.
19. Yin, C.S.; Lee, H.J.; Hong, S.J.; Chung J.H.; Koh, H.G. Microarray analysis of gene expression in chondrosarcoma cells treated with bee venom, Toxicon 2005, 45(1), 81-91.
20. Hu, H.; Chen, D.; Li Y.; Zhang, X. Effect of polypeptides in bee venom on growth inhibition and apoptosis induction of the human hepatoma cell line SMMC-7721 in-vitro and Balb/c nude mice in-vivo. J. Pharm. Pharmacol. 2006, 58(1), 83-89.
21. Moon, D.O.; Park, S.Y.; Heo, M.S.; Kim, K.C.; Park, C.; Ko, W.S. Key regulators in bee venom-induced apoptosis are Bcl-2 and caspase-3 in human leukemic U937 cells through downregulation of ERK and Akt. Int. Immunopharmacol. 2006, 6(12), 1796-1807.
22. Han, S.; Lee, K.; Yeo, J.; Kweon, H.; Woo, S.; Lee, M.; Baek, H.; Kim, S.; Park, K. Effect of honey bee venom on microglial cells nitric oxide and tumor necrosis factor-alpha production stimulated by LPS. J. Ethnopharmacol. 2007, 111(1), 176-181.
23. Lee, Y.J.; Kang, S.J.; Kim, B.M.; Kim, Y.J.; Woo, H.D.; Chung, H.W. Cytotoxicity of honeybee (Apis mellifera) venom in normal human lymphocytes and HL-60 cells. Chem. Biol. Interact. 2007, 169(3), 189-197.
24. Singh, N.P.; McCoy, M.T.; Tice, R.R.; Schneider, L.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell. Res. 1988, 175, 184-191.
25. Collins, A.R. The comet assay for DNA damage and repair: principles, applications, and limitations. Mol. Biotechnol. 2004, 26(3), 249-261.
26. Dixon, D.R.; Pruski, A.M.; Dixon, L.R.J.; Jha, A.N. Marine invertebrate eco-genotoxicology: a methodological overview. Mutagenesis 2002, 17, 495-507.
27. Fairbairn, D.W.; Olive, P.L.; O'Neill, K.L. The Comet Assay: A comprehensive review. Mutat. Res. 1995, 339, 37-59.
28. Lee, R.F.; Steinert, S. Use of the single cell gel electrophoresis/ Comet Assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mutat. Res. 2003, 544, 43-64.
29. Collins, A.; Dusinska, M.; Franklin, M.; Somorovska, M.; Petrovska, H.; Duthie, S.; Fillion, L.; Panayiotidis, M.; Raslová, K.; Vaughan, N. Comet Assay in human biomonitoring studies: reliability, validation, and applications. Environ. Mol. Mutagen. 1997, 30, 139-146.
30. Kumaravel, T.S.; Vilhar, B.; Faux, S.P.; Jha, A.N. Comet Assay measurements: a perspective. Cell. Biol. Toxicol. 2007, (Epub ahead of print) DOI: 10.1007/s10565-007-9043-9.
31. Tice, R.R.; Yager, J.W.; Andrews, P.; Crecelius, E. Effect of hepatic methyl donor status on urinary excretion and DNA damage in B6C3F1 mice treated with sodium arsenite. Mutat. Res. 1997, 386(3), 315-334.
32. Gajski, G.; Ravlic̀, S.; Capuder, Ž.; Garaj-Vrhovac, V. Use of sensitive methods for detection of DNA damage on human lymphocytes exposed to P,P′-DDT: Comet assay and new criteria for scoring micronucleus test. J. Environ. Sci. Health Pt. B, 2007, 42(6), 607-613.
33. Gajski, G.; Garaj-Vrhovac, V. Application of cytogenetic end-points and comet assay on human lymphocytes treated with atorvastatin in vitro. J. Environ. Sci. Health Pt. A, 2008, 43(1), 78-85.
34. Olive, P.; Banáth, J.P.; Durand, R.E. Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells using the comet assay. Radiat. Res. 1990, 122, 86-94.
35. Hellman, B.; Vaghef, H.; Boström, B. The concepts of tail moment and tail inertia in the single cell gel electrophoresis assay. Mutat. Res. 1995, 336, 123-131.
36. Olive, P.L. DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int. J. Radiat. Biol. 1999, 75, 395-405.
37. Piperakis, S.M.; Visvardis, E.E.; Tassiou, A.M. Comet assay for nuclear DNA damage. Meth. Enzymol. 1999, 300, 184-194.