Two Gαi1 rate-modifying mutations act in concert to allow receptor-independent, steady-state measurements of RGS protein activity

Journal of Biomolecular Screening

Volumn 14, Issue 10, 2009, Pages 1195-1206

  Zielinski, Thomas ^a   Kimple, Adam J ^b   Hutsell, Stephanie Q ^b   Koeff, Mark D ^a   Siderovski, David P ^b,c   Lowery, Robert G ^a  

^a BellBrook Labs   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

Fluorescence polarization; GDP detection; Regulators of G protein signaling; Surface plasmon resonance

Indexed keywords

GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; PROTEIN SUBUNIT; RGS PROTEIN; G PROTEIN COUPLED RECEPTOR; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN; MUTANT PROTEIN;

ARTICLE; ENZYME ACTIVITY; FLUORESCENCE POLARIZATION IMMUNOASSAY; HYDROLYSIS; MUTATIONAL ANALYSIS; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; SURFACE PLASMON RESONANCE; BIOASSAY; CHEMISTRY; FLUORESCENCE POLARIZATION; GENETICS; HUMAN; KINETICS; METABOLISM; MOLECULAR LIBRARY; PILOT STUDY; PROTEIN BINDING; PROTEIN SECONDARY STRUCTURE;

BIOLOGICAL ASSAY; FLUORESCENCE POLARIZATION; GTP-BINDING PROTEIN ALPHA SUBUNITS, GI-GO; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; HUMANS; HYDROLYSIS; KINETICS; MUTANT PROTEINS; PILOT PROJECTS; POINT MUTATION; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, G-PROTEIN-COUPLED; RGS PROTEINS; SMALL MOLECULE LIBRARIES;

EID: 71949127119     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057109347473     Document Type: Article

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