A direct fluorescence-based assay for RGS domain GTPase accelerating activity

Analytical Biochemistry

Volumn 340, Issue 2, 2005, Pages 341-351

  Willard, Francis S ^a   Kimple, Adam J ^a   Johnston, Christopher A ^a   Siderovski, David P ^a,b  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

BODIPY; Fluorescence; GAP; GTPase accelerating protein; Heterotrimeric G protein; Regulator of G protein signaling; RGS

Indexed keywords

CATALYSIS; FLUORESCENCE; HYDROLYSIS; NUCLEOTIDES; POSITIVE IONS; PROBES; QUANTUM YIELD; SIGNALING;

BODIPY; DOMAIN-CONTAINING PROTEINS; EXTRACELLULAR SIGNALS; FLUORESCENCE QUANTUM YIELD; G PROTEIN; GUANINE NUCLEOTIDE-BINDING PROTEINS; HETEROTRIMERIC G PROTEINS; NUCLEOTIDE HYDROLYSIS;

PROTEINS;

GUANINE NUCLEOTIDE; GUANOSINE TRIPHOSPHATASE; RGS PROTEIN; RIBOSE;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; ENZYME ACTIVITY; FLUORESCENCE; HYDROLYSIS; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; QUANTUM YIELD; SCREENING; SPECTROSCOPY;

EID: 17444404203     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2005.02.015     Document Type: Article

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