Characterization and optimization of a red-shifted fluorescence polarization ADP detection assay

Assay and Drug Development Technologies

Volumn 7, Issue 1, 2009, Pages 56-67

  Kleman Leyer, Karen M ^a   Klink, Tony A ^a   Kopp, Andrew L ^a   Westermeyer, Thane A ^a   Koeff, Mark D ^a   Larson, Brad R ^a   Worzella, Tracy J ^a   Pinchard, Cori A ^a   Van De Kar, Sebastianus A T ^b   Zaman, Guido J R ^b   Hornberg, Jorrit J ^b   Lowery, Robert G ^a  

^a BellBrook Labs   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; GUANOSINE DIPHOSPHATE; URIDINE DIPHOSPHATE;

ANALYTIC METHOD; ANTIBODY DETECTION; ARTICLE; COMPETITIVE INHIBITION; CONTROLLED STUDY; FLUORESCENCE POLARIZATION IMMUNOASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; IMMUNODETECTION; MICHAELIS CONSTANT; MOLECULAR STABILITY; RECEPTOR AFFINITY; ROOM TEMPERATURE; SCREENING TEST; SIGNAL DETECTION;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; BUFFERS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FLUORESCENCE POLARIZATION IMMUNOASSAY; INDICATORS AND REAGENTS; KINETICS; PEPTIDE LIBRARY; PROTEIN KINASES; REFERENCE STANDARDS;

EID: 65349113053     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2008.175     Document Type: Article

References (34)

1. Sridhar R, Hanson-Painton O, Cooper DR: Protein kinases as therapeutic targets. Pharm Res 2000;17:1345-1353.
2. Cohen P: Protein kinases-the major drug targets of the twenty-first century? Nat Rev Drug Discov 2002;1:309-315.
3. Dancey J, Sausville EA: Issues and progress with protein kinase inhibitors for cancer treatment. Nat Rev Drug Discov 2003;2:296-313.
4. High Throughput Screening 2007: New Strategies, Success Rates, and Use of Enabling Technologies. HighTech Business Decisions, San Jose, CA, 2007.
5. Xu K, Stern AS, Levin W, Chua A, Vassilev LT: A generic time-resolved fluorescence assay for serine/threonine kinase activity: application to Cdc7/Dbf4. J Biochem Mol Biol 2003;36:421-425.
6. Eglen RM, Singh R: Beta galactosidase enzyme fragment complementation as a novel technology for high throughput screening. Comb Chem High Throughput Screen. 2003;6:381-387.
7. Zaman GJ, Garritsen A, de Boer T, van Boeckel CA: Fluorescence assays for high-throughput screening of protein kinases. Comb Chem High Throughput Screen 2003;6:313-320.
8. Gaudet EA, Huang KS, Zhang Y, Huang W, Mark D, Sportsman JR: A homogeneous fluorescence polarization assay adaptable for a range of protein serine/threonine and tyrosine kinases. J Biomol Screen 2003;8:164-175.
9. Xue Q, Wainright A, Gangakhedkar S, Gibbons I: Multiplexed enzyme assays in capillary electrophoretic single-use microfluidic devices. Electrophoresis 2001;22:4000-4007.
10. Burke JR, Pattoli MA, Gregor KR, Brassil PJ, MacMaster JF, Mclntyre KW, et al.: BMS-345541 is a highly selective inhibitor of I kappa B kinase that binds at an allosteric site of the enzyme and blocks NF-kappa B-dependent transcription in mice. J Biol Chem 2003;278:1450-1456.
11. Davidson W, Frego L, Peet GW, Kroe RR, Labadia ME, Lukas SM, et al.: Discovery and characterization of a substrate selective p38alpha inhibitor. Biochemistry 2004;43:11658-11671.
12. Dudley DT, Pang L, Decker SJ, Bridges AJ, Saltiel AR: A synthetic inhibitor of the mitogen-activated protein kinase cascade. Proc Natl Acad Sci U S A 1995;92:7686-7689.
13. Singh P, Harden BJ, Lillywhite BJ, Broad PM: Identification of kinase inhibitors by an ATP depletion method. Assay Drug Dev Technol 2004;2:161-169.
14. Koresawa M, Okabe T: High-throughput screening with quantitation of ATP consumption: a universal non-radioisotope, homogeneous assay for protein kinase. Assay Drug Dev Technol 2004;2:153-160.
15. Walters WP, Namchuk M: Designing screens: how to make your hits a hit. Nat Rev Drug Discov 2003;2:259-266.
16. Charter NW, Kauffman L, Singh R, Eglen RM: A generic, homogenous method for measuring kinase and inhibitor activity via adenosine 5'-diphosphate accumulation. J Biomol Screen 2006;11:390-399.
17. Gibon Y, Blaesing OE, Hannemann J, Carillo P, Hîhne M, Hendriks JH, et al.: A robot-based platform to measure multiple enzyme activities in Arabidopsis using a set of cycling assays: comparison of changes of enzyme activities and transcript levels during diurnal cycles and in prolonged darkness. Plant Cell 2004;16:3304-3325.
18. Schirmer A, Kennedy J, Murli S, Reid R, Santi DV: Targeted covalent inactiva- tion of protein kinases by resorcylic acid lactone polyketides. Proc Natl Acad Sci U S A 2006;103:4234-4239.
19. Lowery RG, Kleman-Leyer K: Transcreener: screening enzymes involved in covalent regulation. Expert Opin Ther Targets 2006;10:179-190.
20. Huss KL, Blonigen PE, Campbell RM: Development of a Transcreener kinase assay for protein kinase A and demonstration of concordance of data with a filter-binding assay format. J Biomol Screen 2007;12:578-584.
21. Harwood HJ Jr, Petras SF, Shelly LD, Zaccaro LM, Perry DA, Makowski MR, et al.: Isozyme-nonselective N-substituted bipiperidylcarboxamide acetyl-CoA carboxylase inhibitors reduce tissue malonyl-CoA concentrations, inhibit fatty acid synthesis, and increase fatty acid oxidation in cultured cells and in experimental animals. J Biol Chem 2003;278:37099-371 1 1.
22. Liu Y, Zalameda L, Kim KW, Wang M, McCarter JD: Discovery of acetyl- coenzyme A carboxylase 2 inhibitors: comparison of a fluorescence intensity- based phosphate assay and a fluorescence polarization-based ADP assay for high-throughput screening. Assay Drug Dev Technol 2007;5:225-235.
23. Tomiya N, Ailor E, Lawrence SM, Betenbaugh MJ, Lee YC: Determination of nucleotides and sugar nucleotides involved in protein glycosylation by high- performance anion-exchange chromatography: sugar nucleotide contents in cultured insect cells and mammalian cells. Anal Biochem 2001;293:129-137.
24. Zhang JH, Chung TD, Oldenburg KR: A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999;4:67-73.
25. 6-carboxymethyl- 5'-AMP conjugates. Use for the quantitation of pyridine nucleotides and of protein-bound ADP-ribose. Biochim Biophys Acta 1981;652:16-28.
26. Richman RA, Kopf GS, Hamet P, Johnson RA: Preparation of cyclic nucleotide antisera with thyroglobulin-cyclic nucleotide conjugates. J Cyclic Nucleotide Res 1980;6:461-468.
27. Knight ZA, Shokat KM: Features of selective kinase inhibitors. Chem Biol 2005;12:621-637.
28. Klink TA, Kleman-Leyer KM, Kopp A, Westermeyer TA, Lowery RG: Evaluating PI3 kinase isoforms using Transcreener ADP assays. J Biomol Screen 2008;13:476-485.
29. Antczak C, Ciro A, Sanchez G, Djaballah H: Development of a high-density fluorescence polarization-based assay for nucleotide-dependent phosphatases using the Transcreener™ technology. http://www.mskcc.org/ mskcc/shared/pdf/Core-Facilities/SBSposter-April2007-TBFP.pdf (accessed December 2008).
30. Zalameda LP, Liu Y, McCarter JD: Application of high throughput fluorescence polarization-based ADP assay for an ATP-dependent group transferase. Poster presented at IBC's 12th Annual DDT World Congress: Building Your Drug Pipeline, August 2007, Boston, MA.
31. Roy P, Roth CM, Margolies MN, Yarmush ML: Effect of pressure on antigen- antibody complexes: modulation by temperature and ionic strength. Mol Immunol 1999;36:1149-1158.
32. Tsumoto K, Ogasahara K, Ueda Y, Watanabe K, Yutani K, Kumagai I: Role of salt bridge formation in antigen-antibody interaction. Entropic contribution to the complex between hen egg white lysozyme and its monoclonal antibody HyHEL10. J Biol Chem 1996;271:32612-32616.
33. Vedvik KL, Eliason HC, Hoffman RL, Gibson JR, Kupcho KR, Somberg RL, et al.: Overcoming compound interference in fluorescence polarization-based kinase assays using far-red tracers. Assay Drug DevTechnol 2004; 2:193-203.
34. Simeonov A, Jadhav A, Thomas CJ, Wang Y, Huang R, Southall NT, et al.: Fluorescence spectroscopic profiling of compound libraries. J Med Chem 2008;51:2363-2371.