Discovery of acetyl-coenzyme A carboxylase 2 inhibitors: Comparison of a fluorescence intensity-based phosphate assay and a fluorescence polarization-based ADP assay for high-throughput screening

Assay and Drug Development Technologies

Volumn 5, Issue 2, 2007, Pages 225-235

  Liu, Yichin ^a   Zalameda, Leeanne ^a   Ki, Won Kim ^a   Wang, Minghan ^a   McCarter, John D ^a  

^a AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYL COENZYME A; ACETYL COENZYME A CARBOXYLASE 2 INHIBITOR; ACETYL COENZYME A1; ACETYL COENZYME A2; ENZYME INHIBITOR; FATTY ACID; MALONYL COENZYME A; UNCLASSIFIED DRUG;

ADIPOSE TISSUE; ANIMAL CELL; ARTICLE; BIOASSAY; COMPARATIVE STUDY; CONTROLLED STUDY; DIABETES MELLITUS; DRUG IDENTIFICATION; DRUG INDICATION; DRUG SCREENING; ENZYME ACTIVITY; ENZYME LOCALIZATION; FATTY ACID OXIDATION; FATTY ACID SYNTHESIS; FLUORESCENCE INTENSITY BASED PHOSPHATE ASSAY; FLUORESCENCE POLARIZATION BASED ADENOSINE PHOSPHATE ASSAY; HEART; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; LIVER; METABOLIC SYNDROME X; NONHUMAN; SKELETAL MUSCLE; TISSUE DISTRIBUTION;

ACETYL-COA CARBOXYLASE; ADENOSINE DIPHOSPHATE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DIMETHYL SULFOXIDE; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; FLUORESCENCE; FLUORESCENCE POLARIZATION IMMUNOASSAY; FLUORESCENT DYES; ISOENZYMES; KINETICS; MALONYL COENZYME A; PHOSPHATES;

EID: 34247589126     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2006.045     Document Type: Article

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