Development and validation of a cytochrome c-coupled assay for pteridine reductase 1 and dihydrofolate reductase

Analytical Biochemistry

Volumn 396, Issue 2, 2010, Pages 194-203

  Shanks, Emma J ^a   Ong, Han B ^a   Robinson, David A ^a   Thompson, Stephen ^a   Sienkiewicz, Natasha ^a   Fairlamb, Alan H ^a   Frearson, Julie A ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

Dihydrofolate reductase; Drug discovery; Pteridine reductase; Screening

Indexed keywords

SCREENING;

DIHYDROFOLATE REDUCTASE; DRUG DISCOVERY; ENZYMATIC REACTION; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATES; PTERIDINE REDUCTASE; SMALL MOLECULE INHIBITOR; STRUCTURE-BASED DESIGNS; TRYPANOSOMA BRUCEI;

ENZYMES;

ANTITRYPANOSOMAL AGENT; CYTOCHROME C; DIHYDROFOLATE REDUCTASE; DIHYDROPTERIDINE REDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ACCURACY; ARTICLE; CONTROLLED STUDY; CRYSTALLOGRAPHY; DRUG RESEARCH; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; ENZYME MECHANISM; ENZYME STRUCTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MICROTITER PLATE ASSAY; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPECTROPHOTOMETRY; TRYPANOSOMA BRUCEI;

TRYPANOSOMA BRUCEI;

EID: 70449729390     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.09.003     Document Type: Article

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