Construction of a homodimeric dihydrofolate reductase-thymidylate synthase bifunctional enzyme

Protein Engineering

Volumn 10, Issue 5, 1997, Pages 567-573

  Trujillo, M ^a   Duncan, R ^a   Santi, D V ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Bifunctional DHFR TS; Engineered DHFR TS; Kinetic channeling; Linker; Substrate channeling

Indexed keywords

BIFUNCTIONAL ENZYME; DIHYDROFOLATE REDUCTASE; FOLIC ACID; THYMIDYLATE SYNTHASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE CONSTRUCT; GENETIC ENGINEERING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; STOP CODON;

ESCHERICHIA COLI; PROTOZOA;

EID: 0030998431     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.5.567     Document Type: Article

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