Newly formed E-cadherin contacts do not activate Cdc42 or induce filopodia protrusion in human keratinocytes

Biology of the Cell

Volumn 102, Issue 1, 2010, Pages 13-24

  Erasmus, Jennifer ^a   Aresta, Sandra ^a,c   Nola, Sebastien ^a   Caron, Emmanuelle ^b   Braga, Vania M M ^a  

^a NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^b Faculty of Natural Sciences   (United Kingdom)

^c INSTITUT DE RECHERCHE POUR LE DÉVELOPPEMENT   (France)

Author keywords

Cadherin receptor; Cdc42; Epithelial cell; Junction assembly; Signalling; Small GTPase

Indexed keywords

GLUTATHIONE TRANSFERASE; NEURAL WISKOTT ALDRICH SYNDROME PROTEIN; PROTEIN CDC42; UVOMORULIN;

ARTICLE; BINDING SITE; CELL CONTACT; CELL JUNCTION; CELL MIGRATION; CELL SPREADING; CONTROLLED STUDY; FILOPODIUM; HUMAN; HUMAN CELL; KERATINOCYTE; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN FUNCTION;

ANIMALS; CADHERINS; CDC42 GTP-BINDING PROTEIN; CELL ADHESION; CELL LINE; CELLS, CULTURED; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; INTERCELLULAR JUNCTIONS; KERATINOCYTES; MICE; PSEUDOPODIA; SIGNAL TRANSDUCTION;

EID: 70350700677     PISSN: 02484900     EISSN: 1768322X     Source Type: Journal    
DOI: 10.1042/BC20090048     Document Type: Article

References (54)

1. Adams, J.C. (2004) Roles of fascin in cell adhesion and motility. Curr. Opin. Cell Biol. 16, 590-596
2. Adams, C.L. and Nelson, W.J. (1998) Cytomechanics of cadherin-mediated cell-cell adhesion. Curr. Opin. Cell Biol. 10, 572-577
3. Adams, C.L., Nelson, W.J. and Smith, S.J. (1996) Quantitative analysis of cadherin-catenin-actin reorganization during development of cell-cell adhesion. J. Cell Biol. 135, 1899-1911
4. Bailly, M., Ichetovkin, I., Grant, W., Zebda, N., Machesky, L.M., Segall, J.E. and Condeelis, J. (2001) The F-actin side binding activity of the Arp2/3 complex is essential for actin nucleation and lamellipod extension. Curr. Biol. 11, 620-625
5. Betson, M., Lozano, E., Zhang, J. and Braga, V.M.M. (2002) Rac activation upon cell-cell contact formation is dependent on signaling from the epidermal growth factor receptor. J. Biol. Chem. 277, 36962-36969
6. Braga, V.M.M. (2002) Cell-cell adhesion and signalling. Curr. Opin. Cell Biol. 14, 546-556
7. Braga, V.M. and Yap, A.S. (2005) The challenges of abundance: epithelial junctions and small GTPase signalling. Curr. Opin. Cell Biol. 17, 466-474
8. Braga, V.M.M., Machesky, L.M., Hall, A. and Hotchin, N.A. (1997) The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts. J. Cell Biol. 137, 1421-1431
9. Bruewer, M., Hopkins, A.M., Hobert, M.E., Nusrat, A. and Madara, J.L. (2004) RhoA, Rac1, and Cdc42 exert distinct effects on epithelial barrier via selective structural and biochemical modulation of junctional proteins and F-actin. Am. J. Physiol. Cell Physiol. 287, C327-C335
10. Burbelo, P.D., Drechsel, D. and Hall, A. (1995) A conserved binding motif defines numerous candidate target proteins for both Cdc42 and Rac GTPases. J. Biol. Chem. 270, 29071-29074
11. Caron, E. and Hall, A. (1998) Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases. Science 282, 1717-1721
12. Desai, R.A., Gao, L., Raghavan, S., Liu, W.F. and Chen, C.S. (2009) Cell polarity triggered by cell-cell adhesion via E-cadherin. J. Cell Sci. 122, 905-911
13. Du, D., Pedersen, E., Wang, Z., Karlsson, R., Chen, Z., Wu, X. and Brakebusch, C. (2008) Cdc42 is crucial for the maturation of primordial cell junctions in keratinocytes independent of Rac1. Exp. Cell Res. 315, 1480-1489
14. Ehrlich, J.S., Hansen, M.D. and Nelson, W.J. (2002) Spatio-temporal regulation of Rac1 localization and lamellipodia dynamics during epithelial cell-cell adhesion. Dev. Cell 3, 259-270
15. Etienne-Manneville, S. and Hall, A. (2001) Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCζ. Cell 106, 489-496
16. Gloushkankova, N.A., Alieva, N.A., Krendel, M.F., Bonder, E.M., Feder, H.H., Vasiliev, J.M. and Gelfand, I.M. (1997) Cell-cell contact changes the dynamics of lamellar activity in non-transformed epitheliocytes but not in their ras-transformed descendants. Proc. Natl. Acad. Sci. U.S.A. 94, 879-883
17. Gomes, E.R., Jani, S. and Gundersen, G.G. (2005) Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells. Cell 121, 451-463
18. Hirai, Y., Nose, A., Kobayashi, S. and Takeichi, M. (1989) Expression and role of E- and P-cadherin adhesion molecules in embryonic histogenesis. I. Lung epithelial morphogenesis. Development 105, 263-270
19. Hodivala, K.J. and Watt, F.M. (1994) Evidence that cadherins play a role in the downregulation of integrin expression that occurs during keratinocyte terminal differentiation. J. Cell Biol. 124, 589-600
20. Hoppe, A.D. and Swanson, J.A. (2004) Cdc42, Rac1, and Rac2 display distinct patterns of activation during phagocytosis. Mol. Biol. Cell 15, 3509-3519
21. Izumi, G., Sakisaka, T., Baba, T., Tanaka, S., Morimoto, K. and Takai, Y. (2004) Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments. J. Cell Biol. 166, 237-248
22. Jacinto, A., Wood, W., Balayo, T., Turmaine, M., Martinez-Arias, A. and Martin, P. (2000) Dynamic actin-based epithelial adhesion and cell matching during Drosophila dorsal closure. Curr. Biol. 10, 1420-1426
23. Jaffe, A.B. and Hall, A. (2005) Rho GTPases: biochemistry and biology. Ann. Rev. Cell Dev. Biol. 21, 247-269
24. Kim, S.H., Zhigang, L. and Sacks, D.B. (2000) E-cadherin-mediated cell-cell attachment activates Cdc42. J. Biol. Chem. 275, 36999-37005
25. Kodama, A., Takaishi, K., Nakano, K., Nishioka, H. and Takai, Y. (1999) Involvement of Cdc42 small G protein in cell-cell adhesion, migration and morphology of MDCK cells. Oncogene 18, 3996-4006
26. Krendel, M.F. and Bonder, E.M. (1999) Analysis of actin filament bundle dynamics during contact formation in live epithelial cells. Cell Motil. Cytoskeleton 43, 296-309
27. Mallavarapu, A. and Mitchison, T. (1999) Regulated actin cytoskeleton assembly at filopodium tips controls their extension and retraction. J. Cell Biol. 146, 1097-1106
28. Martin-Belmonte, F. and Mostov, K. (2008) Regulation of cell polarity during epithelial morphogenesis. Curr. Opin. Cell Biol. 20, 227-234
29. McNeill, H., Ryan, T.A., Smith, S.J. and Nelson, J.W. (1993) Spatial and temporal dissection of immediate and early events following cadherin-mediated epithelial cell adhesion. J. Cell Biol. 120, 1217-1226
30. Mege, R.M., Gavard, J. and Lambert, M. (2006) Regulation of cell-cell junctions by the cytoskeleton. Curr. Opin. Cell Biol. 18, 541-548
31. Musch, A., Cohen, D., Kreitzer, G. and Rodriguez-Boulan, E. (2001) Cdc42 regulates the exit of apical and basolateral proteins from the trans-Golgi network. EMBO J. 20, 2171-2179
32. Noren, N.K., Niessen, C.M., Gumbiner, B.M. and Burridge, K. (2001) Cadherin engagement regulates Rho family GTPases. J. Biol. Chem. 276, 33305-33308
33. Otani, T., Ichii, T., Aono, S. and Takeichi, M. (2006) Cdc42 GEF Tuba regulates the junctional configuration of simple epithelial cells. J. Cell Biol. 175, 135-146
34. Patel, J.C., Hall, A. and Caron, E. (2002) Vav regulates activation of Rac but not Cdc42 during FcγR-mediated phagocytosis. Mol. Biol. Cell 13, 1215-1226
35. Pollard, T.D., Blanchoin, L. and Mullins, R.D. (2000) Molecular mechanisms controlling actin filament dynamics in non muscle cells. Annu. Rev. Biophys. Biomol. Struct. 29, 545-576
36. Raich, W.B., Agbunag, C. and Hardin, J. (1999) Rapid epithelial-sheet sealing in the Caenorhabditis elegans embryo requires cadherin-dependent filopodial priming. Curr. Biol. 9, 1139-1146
37. Rheinwald, J.G. (1989) Methods for clonal growth and serial cultivation of normal human epidermal keratinocytes and mesothelial cells, In Cell Growth and Division: A Practical Approach (Baserga, R., ed.) pp. 81-94, IRL Press, Oxford
38. Rojas, R., Ruiz, W.G., Leung, S.-M., Jou, T.-S. and Apodaca, G. (2001) Cdc42-dependent modulation of tight junctions and membrane protein traffic in polarized Madin-Darby canine kidney cells. Mol. Biol. Cell 12, 2257-2274
39. 2+-dependent cell-cell adhesion molecule homologous to mouse placental cadherin: its low expression in human placental tissues. J. Cell Biol. 109, 1787-1794
40. Stoffler, H.E., Honnert, U., Bauer, C.A., Hofer, D., Schwarz, H., Muller, R.T., Drenckhahn, D. and Bahler, M. (1998) Targeting of the myosin-I myr 3 to intercellular adherens type junctions induced by dominant active Cdc42 in HeLa cells. J. Cell Sci. 111, 2779-2788
41. Svitkina, T.M. and Borisy, G.G. (1999) Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array lamellipodia. J. Cell Biol. 145, 1009-1026
42. Svitkina, T.M., Bulanova, E.A., Chaga, O.Y., Vignjevic, D.M., Kojima, S., Vasiliev, J.M. and Borisy, G.G. (2003) Mechanism of filopodia initiation by reorganization of a dendritic network. J. Cell Biol. 160, 409-421
43. Takai, Y., Miyoshi, J., Ikeda, W. and Ogita, H. (2008) Nectins and nectin-like molecules: roles in contact inhibition of cell movement and proliferation. Nat. Rev. Mol. Cell Biol. 9, 603-615
44. Takaishi, K., Sasaki, T., Kotani, H., Nishioka, H. and Takai, Y. (1997) Regulation of cell-cell adhesion by Rac and Rho small G proteins in MDCK cells. J. Cell Biol. 139, 1047-1059
45. Theriot, J.A. and Mitchison, T.J. (1991) Actin microfilament dynamics in locomoting cells. Nature 352, 126-131
46. Tzima, E., Kiosses, W.B., del Pozo, M.A. and Schwartz, M.A. (2003) Localized Cdc42 activation, detected using a novel assay, mediates microtubule organizing center positioning in endothelial cells in response to fluid shear stress. J. Biol. Chem. 278, 31020-31023
47. Vasioukhin, V., Bauer, C., Yin, M. and Fuchs, E. (2000) Directed actin polymerization is the driving force for epithelial cell-cell adhesion. Cell 100, 209-219
48. Vignjevic, D., Yarar, D., Welch, M.D., Peloquin, J., Svitkina, T. and Borisy, G.G. (2003) Formation of filopodia-like bundles in vitro from a dendritic network. J. Cell Biol. 160, 951-962
49. Vignjevic, D., Kojima, S., Aratyn, Y., Danciu, O., Svitkina, T. and Borisy, G.G. (2006) Role of fascin in filopodial protrusion. J. Cell Biol. 174, 863-875
50. Wu, X., Quondamatteo, F. and Brakebusch, C. (2006) Cdc42 expression in keratinocytes is required for the maintenance of the basement membrane in skin. Matrix Biol. 25, 466-474
51. Wu, X., Li, S., Chrostek-Grashoff, A., Czuchra, A., Meyer, H., Yurchenco, P.D. and Brakebusch, C. (2007) Cdc42 is crucial for the establishment of epithelial polarity during early mammalian development. Dev. Dyn. 236, 2767-2778
52. Yamada, S. and Nelson, W.J. (2007) Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell-cell adhesion. J. Cell Biol. 178, 517-527
53. Yonemura, S., Itoh, M., Nagafuchi, A. and Tsukita, S. (1995) Cell-to-cell adherens junction formation and actin filament organization: similarities and differences between non-polarized fibroblasts and polarized epithelial cells. J. Cell Sci. 108, 127-142
54. Zhang, J., Betson, M., Erasmus, J., Zeikos, K., Bailly, M., Cramer, L. and Braga, V.M.M. (2005) Actin at cell-cell junctions is composed of two dynamic and functional populations. J. Cell Sci. 118, 5549-5562