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PAR3 is essential for cyst-mediated epicardial development by establishing apical cortical domains
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In this article the authors show that disruption of the mouse Par3 gene results in embryonic lethality with defective epicardial development. PAR3-deficient epicardial progenitor cells do not form cell cysts and show defects in the establishment of apical cortical domains, but not in basolateral domains.
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In this article the authors show that BIM is a crucial regulator of apoptosis in vivo. However, cells KD for BIM that fill the luminal space are eventually cleared from the adjacent ducts by a caspase-independent death process.
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Mailleux A.A., Overholtzer M., Schmelzle T., Bouillet P., Strasser A., and Brugge J.S. BIM regulates apoptosis during mammary ductal morphogenesis, and its absence reveals alternative cell death mechanisms. Dev Cell 12 (2007) 221-234. In this article the authors show that BIM is a crucial regulator of apoptosis in vivo. However, cells KD for BIM that fill the luminal space are eventually cleared from the adjacent ducts by a caspase-independent death process.
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Here, the authors address an interesting question elusive for a long time: how endothelial tubes assemble in vivo? To address it; they use high-resolution time-lapse two-photon imaging in zebrafish. The striking movies and data provide strong support for a model in which the formation and intracellular and intercellular fusion of endothelial vacuoles drives vascular lumen formation.
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Kamei M., Saunders W.B., Bayless K.J., Dye L., Davis G.E., and Weinstein B.M. Endothelial tubes assemble from intracellular vacuoles in vivo. Nature 442 (2006) 453-456. Here, the authors address an interesting question elusive for a long time: how endothelial tubes assemble in vivo? To address it; they use high-resolution time-lapse two-photon imaging in zebrafish. The striking movies and data provide strong support for a model in which the formation and intracellular and intercellular fusion of endothelial vacuoles drives vascular lumen formation.
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Bayless K.J., and Davis G.E. The Cdc42 and Rac1 GTPases are required for capillary lumen formation in three-dimensional extracellular matrices. J Cell Sci 115 (2002) 1123-1136
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Li B.X., Satoh A.K., and Ready D.F. Myosin V, Rab11, and dRip11 direct apical secretion and cellular morphogenesis in developing Drosophila photoreceptors. J Cell Biol 177 (2007) 659-669
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On the basis of the studies in MDCK cells in 2D, Rab8 was originally thought to control delivery of proteins from the TGN to the basolateral surface. Here the authors knocked out Rab8 in mice and found that delivery of proteins from the TGN to the apical surface of intestinal cells was affected. A similar defect occurs in a type of human microvillus inclusion disease.
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Sato T., Mushiake S., Kato Y., Sato K., Sato M., Takeda N., Ozono K., Miki K., Kubo Y., Tsuji A., et al. The Rab8 GTPase regulates apical protein localization in intestinal cells. Nature 448 (2007) 366-369. On the basis of the studies in MDCK cells in 2D, Rab8 was originally thought to control delivery of proteins from the TGN to the basolateral surface. Here the authors knocked out Rab8 in mice and found that delivery of proteins from the TGN to the apical surface of intestinal cells was affected. A similar defect occurs in a type of human microvillus inclusion disease.
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Nature
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Sequential pulses of apical epithelial secretion and endocytosis drive airway maturation in Drosophila
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The authors characterize three cellular mechanisms required for epithelial tracheal tube formation and maturation. Initially, a secretion burst deposits proteins into the lumen. Sar1 is required for protein secretion, luminal matrix assembly, and diametric tube expansion. Then, solid luminal material must be rapidly cleared from the tubes and shortly thereafter liquid is removed. Rab5-dependent endocytic activity internalizes and clears the luminal contents.
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Tsarouhas V., Senti K.A., Jayaram S.A., Tiklova K., Hemphala J., Adler J., and Samakovlis C. Sequential pulses of apical epithelial secretion and endocytosis drive airway maturation in Drosophila. Dev Cell 13 (2007) 214-225. The authors characterize three cellular mechanisms required for epithelial tracheal tube formation and maturation. Initially, a secretion burst deposits proteins into the lumen. Sar1 is required for protein secretion, luminal matrix assembly, and diametric tube expansion. Then, solid luminal material must be rapidly cleared from the tubes and shortly thereafter liquid is removed. Rab5-dependent endocytic activity internalizes and clears the luminal contents.
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Dev Cell
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Rac1 is required for reorientation of polarity and lumen formation through a PI 3-kinase-dependent pathway
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Liu K.D., Datta A., Yu W., Brakeman P.R., Jou T.S., Matthay M.A., and Mostov K.E. Rac1 is required for reorientation of polarity and lumen formation through a PI 3-kinase-dependent pathway. Am J Physiol Renal Physiol 293 (2007) F1633-F1640
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