Effects of PI3K catalytic subunit and Akt isoform deficiency on mTOR and p70S6K activation in myoblasts

Biochemical and Biophysical Research Communications

Volumn 390, Issue 2, 2009, Pages 252-257

  Matheny Jr , Ronald W ^a,b   Adamo, Martin L ^a,b  

^a Mays Cancer Center at UT Health San Antonio   (United States)

^b UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

Akt; mTOR; Myoblast; PI3K; S6K

Indexed keywords

MAMMALIAN TARGET OF RAPAMYCIN; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN AKT1; PROTEIN AKT3; PROTEIN KINASE B; PROTEIN KINASE B BETA; PROTEIN P110; PROTEIN P110BETA; S6 KINASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; MOUSE; MYOBLAST; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; CARRIER PROTEINS; CATALYTIC DOMAIN; ENZYME ACTIVATION; GENE KNOCKDOWN TECHNIQUES; INSULIN-LIKE GROWTH FACTOR I; MICE; MYOBLASTS; PHOSPHATIDYLINOSITOL 3-KINASES; PHOSPHORYLATION; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PROTEIN ISOFORMS; PROTO-ONCOGENE PROTEINS C-AKT; RIBOSOMAL PROTEIN S6 KINASES, 70-KDA; TOR SERINE-THREONINE KINASES;

EID: 70350135233     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.09.100     Document Type: Article

References (39)

1. Manning B.D., and Cantley L.C. AKT/PKB signaling: navigating downstream. Cell 129 (2007) 1261-1274
2. Vanhaesebroeck B., and Waterfield M.D. Signaling by distinct classes of phosphoinositide 3-kinases. Exp. Cell Res. 253 (1999) 239-254
3. Alessi D.R., James S.R., Downes C.P., Holmes A.B., Gaffney P.R., Reese C.B., and Cohen P. Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha. Curr. Biol. 7 (1997) 261-269
4. Hirsch E., Costa C., and Ciraolo E. Phosphoinositide 3-kinases as a common platform for multi-hormone signaling. J. Endocrinol. 194 (2007) 243-256
5. Foukas L.C., Claret M., Pearce W., Okkenhaug K., Meek S., Peskett E., Sancho S., Smith A.J., Withers D.J., and Vanhaesebroeck B. Critical role for the p110alpha phosphoinositide-3-OH kinase in growth and metabolic regulation. Nature 441 (2006) 366-370
6. Knight Z.A., Gonzalez B., Feldman M.E., Zunder E.R., Goldenberg D.D., Williams O., Loewith R., Stokoe D., Balla A., Toth B., Balla T., Weiss W.A., Williams R.L., and Shokat K.M. A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling. Cell 125 (2006) 733-747
7. Guillermet-Guibert J., Bjorklof K., Salpekar A., Gonella C., Ramadani F., Bilancio A., Meek S., Smith A.J., Okkenhaug K., and Vanhaesebroeck B. The p110beta isoform of phosphoinositide 3-kinase signals downstream of G protein-coupled receptors and is functionally redundant with p110gamma. Proc. Natl. Acad. Sci. USA 105 (2008) 8292-8297
8. Jia S., Liu Z., Zhang S., Liu P., Zhang L., Lee S.H., Zhang J., Signoretti S., Loda M., Roberts T.M., and Zhao J.J. Essential roles of PI(3)K-p110beta in cell growth, metabolism and tumorigenesis. Nature 454 (2008) 776-779
9. Marques M., Kumar A., Poveda A.M., Zuluaga S., Hernandez C., Jackson S., Pasero P., and Carrera A.C. Specific function of phosphoinositide 3-kinase beta in the control of DNA replication. Proc. Natl. Acad. Sci. USA 106 (2009) 7525-7530
10. Ciraolo E., Iezzi M., Marone R., Marengo S., Curcio C., Costa C., Azzolino O., Gonella C., Rubinetto C., Wu H., Dastru W., Martin E.L., Silengo L., Altruda F., Turco E., Lanzetti L., Musiani P., Ruckle T., Rommel C., Backer J.M., Forni G., Wymann M.P., and Hirsch E. Phosphoinositide 3-kinase p110beta activity: key role in metabolism and mammary gland cancer but not development. Sci. Signal. 1 (2008) ra3
11. Brunet A., Bonni A., Zigmond M.J., Lin M.Z., Juo P., Hu L.S., Anderson M.J., Arden K.C., Blenis J., and Greenberg M.E. Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Cell 96 (1999) 857-868
12. Kim A.H., Khursigara G., Sun X., Franke T.F., and Chao M.V. Akt phosphorylates and negatively regulates apoptosis signal-regulating kinase 1. Mol. Cell. Biol. 21 (2001) 893-901
13. Datta S.R., Dudek H., Tao X., Masters S., Fu H., Gotoh Y., and Greenberg M.E. Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91 (1997) 231-241
14. Dummler B., and Hemmings B.A. Physiological roles of PKB/Akt isoforms in development and disease. Biochem. Soc. Trans. 35 (2007) 231-235
15. Cho H., Thorvaldsen J.L., Chu Q., Feng F., and Birnbaum M.J. Akt1/PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice. J. Biol. Chem. 276 (2001) 38349-38352
16. Cho H., Mu J., Kim J.K., Thorvaldsen J.L., Chu Q., Crenshaw III E.B., Kaestner K.H., Bartolomei M.S., Shulman G.I., and Birnbaum M.J. Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta). Science 292 (2001) 1728-1731
17. Easton R.M., Cho H., Roovers K., Shineman D.W., Mizrahi M., Forman M.S., Lee V.M., Szabolcs M., de Jong R., Oltersdorf T., Ludwig T., Efstratiadis A., and Birnbaum M.J. Role for Akt3/protein kinase Bgamma in attainment of normal brain size. Mol. Cell. Biol. 25 (2005) 1869-1878
18. Liu X., Shi Y., Birnbaum M.J., Ye K., De Jong R., Oltersdorf T., Giranda V.L., and Luo Y. Quantitative analysis of anti-apoptotic function of Akt in Akt1 and Akt2 double knock-out mouse embryonic fibroblast cells under normal and stressed conditions. J. Biol. Chem. 281 (2006) 31380-31388
19. Sale E.M., Hodgkinson C.P., Jones N.P., and Sale G.J. A new strategy for studying protein kinase B and its three isoforms. Role of protein kinase B in phosphorylating glycogen synthase kinase-3, tuberin, WNK1, and ATP citrate lyase. Biochemistry 45 (2006) 213-223
20. Chin Y.R., and Toker A. Function of Akt/PKB signaling to cell motility, invasion and the tumor stroma in cancer. Cell. Signal. 21 (2009) 470-476
21. Matheny Jr. R.W., and Adamo M.L. Role of Akt isoforms in IGF-I-mediated signaling and survival in myoblasts. Biochem. Biophys. Res. Commun. 389 (2009) 117-121
22. Pende M. MTOR, Akt, S6 kinases and the control of skeletal muscle growth. Bull. Cancer 93 (2006) E39-E43
23. Kovacina K.S., Park G.Y., Bae S.S., Guzzetta A.W., Schaefer E., Birnbaum M.J., and Roth R.A. Identification of a proline-rich Akt substrate as a 14-3-3 binding partner. J. Biol. Chem. 278 (2003) 10189-10194
24. Inoki K., Li Y., Zhu T., Wu J., and Guan K.L. TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling. Nat. Cell Biol. 4 (2002) 648-657
25. Sarbassov D.D., Guertin D.A., Ali S.M., and Sabatini D.M. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science 307 (2005) 1098-1101
26. Alessi D.R., Pearce L.R., and Garcia-Martinez J.M. New insights into mTOR signaling: mTORC2 and beyond. Sci. Signal. 2 (2009) pe27
27. Guertin D.A., and Sabatini D.M. The pharmacology of mTOR inhibition. Sci. Signal. 2 (2009) pe24
28. Chiang G.G., and Abraham R.T. Phosphorylation of mammalian target of rapamycin (mTOR) at Ser-2448 is mediated by p70S6 kinase. J. Biol. Chem. 280 (2005) 25485-25490
29. Holz M.K., and Blenis J. Identification of S6 kinase 1 as a novel mammalian target of rapamycin (mTOR)-phosphorylating kinase. J. Biol. Chem. 280 (2005) 26089-26093
30. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
31. Brognard J., Sierecki E., Gao T., and Newton A.C. PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Mol. Cell 25 (2007) 917-931
32. Marques M., Kumar A., Cortes I., Gonzalez-Garcia A., Hernandez C., Moreno-Ortiz M.C., and Carrera A.C. Phosphoinositide 3-kinases p110alpha and p110beta regulate cell cycle entry, exhibiting distinct activation kinetics in G1 phase. Mol. Cell. Biol. 28 (2008) 2803-2814
33. Sancak Y., Thoreen C.C., Peterson T.R., Lindquist R.A., Kang S.A., Spooner E., Carr S.A., and Sabatini D.M. PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Mol. Cell 25 (2007) 903-915
34. Vander Haar E., Lee S.I., Bandhakavi S., Griffin T.J., and Kim D.H. Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40. Nat. Cell Biol. 9 (2007) 316-323
35. Fan Q.W., Cheng C., Knight Z.A., Haas-Kogan D., Stokoe D., James C.D., McCormick F., Shokat K.M., and Weiss W.A. EGFR signals to mTOR through PKC and independently of Akt in glioma. Sci. Signal. 2 (2009) ra4
36. Wlodarski P., Kasprzycka M., Liu X., Marzec M., Robertson E.S., Slupianek A., and Wasik M.A. Activation of mammalian target of rapamycin in transformed B lymphocytes is nutrient dependent but independent of Akt, mitogen-activated protein kinase/extracellular signal-regulated kinase kinase, insulin growth factor-I, and serum. Cancer Res. 65 (2005) 7800-7808
37. Brewer C., Yeager N., and Di Cristofano A. Thyroid-stimulating hormone initiated proliferative signals converge in vivo on the mTOR kinase without activating AKT. Cancer Res. 67 (2007) 8002-8006
38. O'Neil T.K., Duffy L.R., Frey J.W., and Hornberger T.A. The role of phosphoinositide 3-kinase and phosphatidic acid in the regulation of mammalian target of rapamycin following eccentric contractions. J. Physiol. 587 (2009) 3691-3701
39. Hornberger T.A., Chu W.K., Mak Y.W., Hsiung J.W., Huang S.A., and Chien S. The role of phospholipase D and phosphatidic acid in the mechanical activation of mTOR signaling in skeletal muscle. Proc. Natl. Acad. Sci. USA 103 (2006) 4741-4746