Bone and Wound Healing Augmentation with Platelet-Rich Plasma

Clinics in Podiatric Medicine and Surgery

Volumn 26, Issue 4, 2009, Pages 559-588

  Smith, Simon E ^a,b   Roukis, Thomas S ^c  

^a Western Australian Branch   (Australia)

^b LA TROBE UNIVERSITY   (Australia)

^c MADIGAN ARMY MEDICAL CENTER   (United States)

Author keywords

Autologous platelet poor plasma; Autologous platelet rich plasma; Bone graft; Ulceration; Wound coverage

Indexed keywords

ACID CITRATE DEXTROSE; BONE MORPHOGENETIC PROTEIN 2; EPIDERMAL GROWTH FACTOR; OSTEOCALCIN; OSTEOPONTIN; PADGEM PROTEIN; PLATELET DERIVED GROWTH FACTOR; PLATELET DERIVED GROWTH FACTOR B; RECOMBINANT PLATELET DERIVED GROWTH FACTOR; RECOMBINANT PLATELET DERIVED GROWTH FACTOR BB; SOMATOMEDIN C; SULFADIAZINE SILVER; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA1; VASCULOTROPIN; VASCULOTROPIN 165; VASCULOTROPIN A;

ACHONDROPLASIA; ANGIOGENESIS; ARTHROPLASTY; BONE DEVELOPMENT; BONE GRAFT; BONE MATURATION; BONE REGENERATION; CLINICAL TRIAL; CONGENITAL PSEUDARTHROSIS; DIABETIC FOOT; DISTRACTION OSTEOGENESIS; EDENTULOUSNESS; ENCHONDRAL OSSIFICATION; FRACTURE; FRACTURE HEALING; FRACTURE NONUNION; GRANULATION TISSUE; HEMOSTASIS; HUMAN; HYPOCHONDROPLASIA; JAW MALFORMATION; LEG ULCER; LIMB ISCHEMIA; MANDIBLE RECONSTRUCTION; MAXILLOFACIAL SURGERY; NONHUMAN; ORAL SURGERY; OSTEOBLAST; PRIORITY JOURNAL; REVIEW; SKIN GRAFT; TENDON RECONSTRUCTION; THROMBOCYTE ACTIVATION; THROMBOCYTE AGGREGATION; THROMBOCYTE COUNT; THROMBOCYTE POOR PLASMA; THROMBOCYTE RICH PLASMA; TISSUE REGENERATION; ULCER; WOUND HEALING; WOUND HEALING IMPAIRMENT;

ANIMALS; BONE AND BONES; CARTILAGE; GUIDED TISSUE REGENERATION; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; ORAL SURGICAL PROCEDURES; ORTHOPEDIC PROCEDURES; PLATELET-RICH PLASMA; TENDON INJURIES; TRANSPLANTATION, AUTOLOGOUS; WOUND HEALING;

EID: 70349204331     PISSN: 08918422     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cpm.2009.07.002     Document Type: Review

References (243)

1. Behnke O., and Forer A. From megakaryocytes to platelets: platelet morphogenesis takes place in the bloodstream. Eur J Haematol Suppl 61 1 (1998) 3-23
2. Italiano Jr. J.E., and Hartwig J.H. Megakaryocyte and platelet structure. Chapter 105. In: Hoffman R. (Ed). Hematology: basic principles and practice. 4th Edition (2005), Elsevier, Churchill-Livingstone, Philadelphia p. 1872-80
3. Mehta S., and Watson J.T. Platelet rich concentrate: basic science and current clinical applications. J Orthop Trauma 22 6 (2008) 433-438
4. Pagana K.D., and Pagana T.J. Blood studies: platelet count. Chapter 2. In: Pagana K.D., and Pagana T.J. (Eds). Mosby's Manual of Diagnostic and laboratory Tests (2006), Mosby Elsevier, St Louis (IL) 409-412
5. Harrison P., and Cramer E.M. Platelet alpha-granules. Blood Rev 7 1 (1993) 52-62
6. Plow E.F., and Abrams C.S. Chapter 106-the molecular basis for platelet function. Chapter 106. In: Hoffman R. (Ed). Hematology: basic principles and practice. 4th Edition (2005), Elsevier, Churchill, Livingstone, Philadelphia p. 1881-98
7. Broughton II G., Janis J.E., and Attinger C.E. Wound healing: an overview. Plast Reconstr Surg 117 Suppl 7S (2006) 1eS-32eS
8. Broughton II G., Janis J.E., and Attinger C.E. The basic science of wound healing. Plast Reconstr Surg 117 Suppl (2006) 12S-34S
9. Witte M., and Barbul A. General principles of wound healing. Surg Clin North Am 77 3 (1997) 509-528
10. Diegelman R.F., and Evans M.C. Wound healing: an overview of acute fibrotic and delayed healing. Front Biosci 9 (2004) 283-289
11. Marx R.E. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 62 4 (2004) 489-496
12. Eppley B.L., Woodell J.E., and Higgins J. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast Reconstr Surg 114 11 (2004) 1502-1508
13. Schmidmaier G., Herrmann S., Green J., et al. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation. Bone 39 5 (2006) 1156-1163
14. Weibrich G., Kleis W.K., Hafner G., et al. Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg 30 2 (2002) 97-102
15. Ratnoff O.D., and Davie E.W. Waterfall sequence for intrinsic blood clotting. Science 145 (1964) 1310-1312
16. Froum S.J., Wallace S.S., Tarnow D.P., et al. Effect of platelet-rich plasma on bone growth and osseointegration in human maxillary sinus grafts: three bilateral case reports. Int J Periodontics Restorative Dent 22 1 (2002) 45-53
17. Bennett S.P., Griffiths G.D., Schor A.M., et al. Growth factors in the treatment of diabetic foot ulcers. Br J Surg 90 2 (2003) 133-146
18. Pierce G., Mustoe T., Altrock B., et al. Role of platelet-derived growth factor in wound healing. J Cell Biochem 45 4 (1991) 319-326
19. Canalis E., McCarthy T.L., and Centerlla M. Effects of Platelet-derived growth factor on bone formation in vitro. J Cell Physiol 140 3 (1989) 530-537
20. Heldin C.H., and Westermark B. Mechanism of action and in vivo role of platelet-derived growth factor. Physiol Rev 79 4 (1999) 1283-1316
21. Heldin C.H., and Westermark B. PDGF-like growth factors in autocrine simulation of growth. J Cell Physiol 133 Suppl 5 (1987) 31-34
22. Lin H., Chen B., Sun W., et al. The effect of collagen-targeting platelet-derived growth factor on cellularization and vascularization of collagen scaffolds. Biomaterials 27 33 (2006) 5708-5714
23. Acevedo J.I. Fixation of metatarsal osteotomies in the treatment of hallux valgus. Foot Ankle Clin 5 3 (2000) 451-468
24. Rhee S., and Grinnel F. P21-activated kinase 1: convergence point in PDGF- and LPA-stimulated collagen matrix contraction by human fibroblasts. J Cell Biol 172 3 (2006) 423-432
25. Uutela M., Wirzenius M., Paavonen K., et al. PDGF-D induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Blood 104 10 (2004) 3198-3204
26. Jinnin M., Ihn H., Mimura Y., et al. Regulation of fibrogenic/fibrolytic genes by platelet-derived growth factor C, a novel growth factor, in human dermal fibroblasts. J Cell Biol 202 2 (2005) 510-517
27. Rabhi-Sabile S., Pidard D., Lawler J., et al. Proteolysis of thrombospondin during cathepsin-G-induced platelet aggregation: functional role of the 165-kDa carboxy-terminal fragment. FEBS Lett 386 1 (1996) 82-86
28. Krishnaswami S., Ly Q.P., Rothman V.L., et al. Thrombospondin-1 promotes proliferative healing through stabilization of PDGF. J Surg Res 107 1 (2002) 124-130
29. Ando Y., and Jensen P.J. Epidermal growth factor and insulin-like growth factor I enhance keratinocyte migration. J Invest Dermatol 100 5 (1993) 633-639
30. Stavri G.T., Hong Y., Zachary I.C., et al. Hypoxia and platelet-derived growth factor-BB synergistically upregulate the expression of vascular endothelial growth factor in vascular smooth muscle cells. FEBS Lett 358 3 (1995) 311-315
31. Lindahl P., Johansson B.R., Leveen P., et al. Pericyte loss and microaneurysm formation in PDGF-B-deficient mice. Science 277 5323 (1997) 242-245
32. Sundberg C., Branting M., Gerdin B., et al. Tumor cell and connective tissue cell interactions in human colorectal adenocarcinoma. Transfer of platelet-derived growth factor-AB/BB to stromal cells. Am J Pathol 151 2 (1997) 479-492
33. Grotendorst G.R., Martin G.R., Pencev D., et al. Stimulation of granulation tissue formation by platelet-derived growth factor in normal and diabetic rats. J Clin Invest 76 6 (1985) 2323-2329
34. Pierce G.F., Mustoe T.A., Senior R.M., et al. In vivo incisional wound healing augmented by platelet-derived growth factor and recombinant c-sis gene homodimeric proteins. J Exp Med 167 3 (1988) 974-987
35. Pierce G.F., Mustoe T.A., Lingelbach J., et al. Platelet-derived growth factor and transforming growth factor-beta enhance tissue repair activities by unique mechanisms. J Cell Biol 109 1 (1989) 429-440
36. Sprugel K.H., McPherson J.M., Clowes A.W., et al. Effects of growth factors in vivo. I. Cell ingrowth into porous subcutaneous chambers. Am J Pathol 129 3 (1987) 601-613
37. Mustoe T.A., Pierce G.F., Morishima C., et al. Growth factor-induced acceleration of tissue repair through direct and inductive activities in a rabbit dermal ulcer model. J Clin Invest 87 2 (1991) 694-703
38. Pierce G.F., Veande Berg J., Rudolph R., et al. Platelet-derived growth factor-BB and transforming growth factor beta 1 selectively modulate glycosaminoglycans, collagen, and myofibroblasts in excisional wounds. Am J Pathol 138 3 (1991) 629-646
39. Robson M.C., Phillips L.G., Thomason A., et al. Platelet-derived growth factor BB for the treatment of chronic pressure ulcers. Lancet 339 8784 (1992) 23-25
40. Lynch S.E., Colvin R.B., and Antoniades H.N. Growth factors in wound healing. Single and synergistic effects on partial thickness porcine skin wounds. J Clin Invest 84 2 (1989) 640-646
41. Greenhalgh D.G., Hummel R.P., Albertson S., et al. Synergistic actions of platelet-derived growth factor and the insulin-like growth factors in vivo. Wound Repair Regen 1 2 (1993) 69-81
42. Brown R.L., Breeden M.P., and Greenhalgh D.G. PDGF and TGF-alpha act synergistically to improve wound healing in the genetically diabetic mouse. J Surg Res 56 6 (1994) 562-570
43. Mustoe T.A., Purdy J., Gramates P., et al. Reversal of impaired wound healing in irradiated rats by platelet-derived growth factor-BB. Am J Surg 158 4 (1989) 345-350
44. Greenhalgh D.G., Sprugel K.H., Murray M.J., et al. PDGF and FGF stimulate wound healing in the genetically diabetic mouse. Am J Pathol 136 6 (1990) 1235-1246
45. Uhl E., Rosken F., Sirsjo A., et al. Influence of platelet-derived growth factor on microcirculation during normal and impaired wound healing. Wound Repair Regen 11 5 (2003) 361-367
46. Lepisto J., Laato M., Miinikoski J., et al. Effects of homodimeric isoforms of platelet-derived growth factor (PDGF-AA and PDGF-BB) on wound healing in rat. J Surg Res 53 6 (1992) 596-601
47. Deuel T.F., Kawahara R.S., Mustoe T.A., et al. Growth factors and wound healing: platelet-derived growth factor as a model cytokine. Annu Rev Med 42 (1991) 567-584
48. Sprugel K.H., Greenhalgh D.G., Murray M.J., et al. Platelet-derived growth factor and impaired wound healing. Prog Clin Biol Res 365 (1991) 327-340
49. Doxey D.L., Ng M.C., Dill R.E., et al. Platelet derived growth factor levels in wounds in diabetic rates. Life Sci 57 11 (1995) 1111-1123
50. Embil J.M., Papp K., Sibbald G., et al. Recombinant human platelet-derived growth factor-BB (becaplermin) for healing chronic lower extremity diabetic ulcers: an open-label clinical evaluation of efficacy. Wound Repair Regen 8 3 (2000) 162-168
51. Smiell J.M., Wieman T.J., Steed D.L., et al. Efficacy and safety of becaplermin (recombinant human platelet-derived growth factor-BB) in patients with nonhealing, lower extremity diabetic ulcers: a combined analysis of four randomized studies. Wound Repair Regen 7 5 (1999) 335-346
52. Steed D.L. Clinical evaluation of recombinant human platelet-derived growth factor for the treatment of lower extremity diabetic ulcers. Diabetic Ulcer Study Group. J Vasc Surg 21 1 (1995) 71-78
53. Rees R.S., Robson M.C., Smiell J.M., et al. Becaplermin gel in the treatment of pressure ulcers: a phase II randomized, double-blind, placebo-controlled study. Wound Repair Regen 7 3 (1999) 141-147
54. Wieman T.J., Smiell J.M., and Su Y. Efficacy and safety of a topical gel formulation of recombinant human platelet-derived growth factor-BB (becaplermin) in patients with chronic neuropathic diabetic ulcers. A phase III randomized placebo-controlled double-blind study. Diabetes Care 21 5 (1998) 822-827
55. Andrew J.G., Hoyland J.A., Freemont A.J., et al. Platelet-derived growth factor expression in normally healing human fractures. Bone 16 4 (1995) 455-460
56. Nash T.J., Howlett C.R., Martin C., et al. Effect of platelet-derived growth factor on tibial osteotomies in rabbits. Bone 15 2 (1994) 203-208
57. Wahl S.M., Hunt D.A., Wakefield L.M., et al. Transforming growth factor type beta induces monocyte chemotaxis and growth factor production. Proc Natl Acad Sci U S A 84 16 (1987) 5788-5792
58. Lawrence W., and Diegelmann R. Growth factors in wound healing. Clin Dermatol 12 1 (1994) 157-169
59. Sporn M.B., Roberts A.B., Wakefield L.M., et al. Transforming growth factor-beta: biological function and chemical structure. Science 233 4763 (1986) 532-534
60. Bassols A., and Massague J. Transforming growth factor beta regulates the expression and structure of extracellular chondroitin/dermatan sulfate proteoglycans. J Biol Chem 263 6 (1988) 3039-3045
61. Fukamizu J., and Grinnell F. Spatial organization of extracellular matrix and fibroblast activity: effects of serum, TGF-Beta and Fibronectin. Exp Cell Res 190 2 (1990) 276-282
62. Goldberg M.T., Han Y.P., Yan C., et al. TNF-alpha suppresses alpha-smooth muscle actin expression in human dermal fibroblasts: an implication for abnormal wound healing. J Invest Dermatol 127 11 (2007) 2645-2655
63. Greenwel P., Inagaki Y., Hu W., et al. Sp1 is required for the early response of alpha2(I) collagen to transforming growth factor-beta1. J Biol Chem 272 32 (1997) 19738-19745
64. Mauviel A., Chung K.Y., Agarwal A., et al. Cell-specific induction of distinct oncogenes of the Jun family is responsible for differential regulation of collagenase gene expression by transforming growth factor-beta in fibroblasts and keratinocytes. J Biol Chem 271 18 (1996) 10917-10923
65. Papakonstantinou E., Aletras A.J., Roth M., et al. Hypoxia modulates the effects of transforming growth factor-beta isoforms on matrix-formation by primary human lung fibroblasts. Cytokine 24 1-2 (2003) 25-35
66. Riedel K., Riedel F., Goessler U.R., et al. TGF-beta antisense therapy increases angiogenic potential in human keratinocytes in vitro. Arch Med Res 38 1 (2007) 45-51
67. Ignotz R., Endo T., and Massague J. Regulation of fibronectin and type I collagen mRNA levels by transforming growth factor-beta. J Biol Chem 262 14 (1987) 6443-6446
68. Meckmongkol T.T., Harmon R., McKeown-Longo P., et al. The fibronectin synergy site modulates TGF-beta-dependent fibroblast contraction. Biochem Biophys Res Commun 360 4 (2007) 709-714
69. Cordeiro M.F., Reichel M.B., Gay J.A., et al. Transforming growth factor-beta1, -beta2, and -beta3 in vivo: effects on normal and mitomycin C-modulated conjunctival scarring. Invest Ophthalmol Vis Sci 40 9 (1999) 1975-1982
70. Roberts A.B., Sporn M.B., Assoian R.K., et al. Transforming growth factor type beta: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro. Proc Natl Acad Sci U S A 83 12 (1986) 4167-4171
71. Zeng G., McCue H.M., Mastrangelo L., et al. Endogenous TGF-beta activity is modified during cellular aging: effects on metalloproteinase and TIMP-1 expression. Exp Cell Res 228 2 (1996) 271-276
72. Robey P.G., Young M.F., Flanders K.C., et al. Osteoblasts synthesize and respond to transforming growth factor-type beta (TGF-beta) in vitro. J Cell Biol 105 1 (1987) 457-463
73. Lieberman J.R., Daluiski A., and Einhorn T.A. The role of growth factors in the repair of bone: biology and clinical applications. J Bone Joint Surg Am 84-A 6 (2002) 1032-1044
74. Bolander M.E. Regulation of fracture repair by growth factors. Proc Soc Exp Biol Med 200 2 (1992) 165-170
75. Joyce M.E., Jingushi S., and Bolander M.E. Transforming growth factor-beta in the regulation of fracture repair. Orthop Clin North Am 21 1 (1990) 199-209
76. Bourque W.T., Gross M.T., and Hall B.K. Expression of four growth factors during fracture repair. Int J Dev Biol 37 4 (1993) 573-579
77. Grageda E. Platelet-rich plasma and bone graft materials: a review and a standardized research protocol. Implant Dent 13 4 (2004) 301-309
78. Lind M., Schumacker B., Søballe K., et al. Transforming growth factor-beta enhances fracture healing in rabbit tibiae. Acta Orthop Scand 64 5 (1993) 553-556
79. Nielsen H.M., Andreassen T.T., Ledet T., et al. Local injection of TGF-beta increases the strength of tibial fractures in the rat. Acta Orthop Scand 65 1 (1994) 37-41
80. Critchlow M.A., Bland Y.S., and Ashhurst D.E. The effect of exogenous transforming growth factor-beta 2 on healing fractures in the rabbit. Bone 16 5 (1995) 521-527
81. Beck L.S., Amento E.P., Xu Y., et al. TGF-beta 1 induces bone closure of skull defects: temporal dynamics of bone formation in defects exposed to rhTGF-beta 1. J Bone Miner Res 8 6 (1993) 753-761
82. Beck L.S., Deguzman L., Lee W.P., et al. Rapid publication. TGF-beta 1 induces bone closure of skull defects. J Bone Miner Res 6 11 (1991) 1257-1265
83. Nanney L.B., Magid M., Stoscheck C.M., et al. Comparison of epidermal growth factor binding and receptor distribution in normal human epidermis and epidermal appendages. J Invest Dermatol 83 5 (1984) 385-393
84. Nanney L.B., McKanna J.A., Stoscheck C.M., et al. Visualization of epidermal growth factor receptors in human epidermis. J Invest Dermatol 82 2 (1984) 165-169
85. Grotendorst G., Soma Y., Takehara K., et al. EGF and TGF-alpha are potent chemoattractants for endothelial cells and EGF-like peptides are present at sites of tissue regeneration. J Clin Invest 139 3 (1989) 617-623
86. Tokumaru S., Higashiyama S., Endo T., et al. Ectodomain shedding of epidermal growth factor receptor ligands is required for keratinocyte migration in cutaneous wound healing. J Cell Biol 151 2 (2000) 209-220
87. Nanney L.B. Epidermal and dermal effects of epidermal growth factor during wound repair. J Invest Dermatol 94 5 (1990) 624-629
88. Reiss M., and Sartorelli A.C. Regulation of growth and differentiation of human keratinocytes by type beta transforming growth factor and epidermal growth factor. Cancer Res 47 24 Pt 1 (1987) 6705-6709
89. Brown G.L., Nanney L.B., Griffen J., et al. Enhancement of wound healing by topical treatment with epidermal growth factor. N Engl J Med 321 2 (1989) 76-79
90. Falanga V., Eaglstein W.H., Bucalo B., et al. Topical use of human recombinant epidermal growth factor (h-EGF) in venous ulcers. J Dermatol Surg Oncol 18 7 (1992) 604-606
91. Viswanathan V., Pendsey S., Sekar N., et al. A Phase III Study to Evaluate the Safety and Efficacy of Recombinant Human Epidermal Growth Factor (REGEN-D™ 150) in Healing Diabetic foot ulcers. Wounds 18 7 (2006) 186-196
92. Grant M., Jerdan J., and Merimee T. Insulin-like growth factor-I modulates endothelial cell chemotaxis. J Clin Endocrinol Metab 65 2 (1987) 370-371
93. Clemmons D.R., Maile L.A., Ling Y., et al. Role of the integrin alphaVbeta3 in mediating increased smooth muscle cell responsiveness to IGF-I in response to hyperglycemic stress. Growth Horm IGF Res 17 4 (2007) 265-270
94. Panagakos F.S. Insulin-like growth factors-I and -II stimulate chemotaxis of osteoblasts isolated from fetal rat calvaria. Biochimie 75 11 (1993) 991-994
95. Lynch S.E., Nixon J.C., Colvin R.B., et al. Role of platelet derived growth factors in wound healing: synergistic effects with other growth factors. Proc Natl Acad Sci U S A 84 21 (1987) 7696-7700
96. Guvakova M.A. Insulin-like growth factors control cell migration in health and disease. Int J Biochem Cell Biol 39 5 (2007) 890-909
97. Clemens T.L., and Chernausek S.D. Genetic strategies for elucidating insulin-like growth factor action in bone. Growth Horm IGF Res 14 3 (2004) 195-199
98. Niu T., and Rosen C.J. The insulin-like growth factor-I gene and osteoporosis: a critical appraisal. Gene 361 (2005) 38-56
99. Canalis E., Rydziel S., Delany A.M., et al. Insulin-like growth factors inhibit interstitial collagenase synthesis in bone cell cultures. Endocrinology 136 4 (1995) 1348-1354
100. Jonsson K.B., Ljunghall S., Karlström O., et al. Insulin-like growth factor 1 enhances the formation of type 1 collagen in hydrocortisone-treated human osteoblasts. Biosci Rep 13 5 (1993) 297-302
101. Mohan S., and Baylink D.J. Insulin-like growth factor system components and the coupling of bone formation to resorption. Horm Res 45 Suppl 1 (1996) 59-62
102. Rosen C.J., Hunter L.R., and Donahue S.J. Insulin-like growth factors and bone: the osteoporosis connection revisited. Proc Soc Exp Biol Med 206 2 (1994) 83-102
103. Eingartner C., Coerper s., Fritz J., et al. Growth factors in distraction osteogenesis. Immuno-histological pattern of TGF-beta1 and IGF-I in human callus induced by distraction osteogenesis. Int Orthop 23 5 (1999) 253-259
104. Okazaki K., Jingushi S., Ikenoue T., et al. Expression of parathyroid hormone-related peptide and insulin-like growth factor I during rat fracture healing. J Orthop Res 21 3 (2003) 511-520
105. Bak B., Jorgensen P.H., and Andreassen T.T. Dose response of growth hormone on fracture healing in the rat. Acta Orthop Scand 61 1 (1990) 54-57
106. Andrew J.G., Hoyland J., Freemont A.J., et al. Insulinlike growth factor gene expression in human fracture callus. Calcif Tissue Int 53 2 (1993) 97-102
107. Schmidmaier G., Wildemann B., Bail H., et al. Local application of growth factors (insulin-like growth factor-1 and transforming growth factor-beta1) from a biodegradable poly(D, L-lactide) coating of osteosynthetic implants accelerates fracture healing in rats. Bone 28 4 (2001) 341-350
108. Thaller S.R., Dart A., and Tesluk H. The effects of insulin-like growth factor-1 on critical-size calvarial defects in Sprague-Dawley rats. Ann Plast Surg 31 5 (1993) 429-433
109. Raschke M., Wildemann B., Inden P., et al. Insulinlike growth factor-1 and transforming growth factor-beta1 accelerates osteotomy healing using polylactide-coated implants as a delivery system: a biomechanical and histological study in minipigs. Bone 30 1 (2002) 144-151
110. Fowlkes J.L., Thrailkill K.M., Liu L., et al. Effects of systemic and local administration of recombinant human IGF-I (rhIGF-I) on de novo bone formation in an aged mouse model. J Bone Miner Res 21 9 (2006) 1359-1366
111. Zhou H., Dong Q., Zhou X., et al. Calvarial defects repair in nude mice by human bone marrow mesenchymal stem cells transfected with insulin like growth factor-1. Bone 43 (2008) S60
112. Banks R.E., Forbes M.A., Kinsey S.E., et al. Release of angiogenic cytokine vascular endothelial growth factor (VEGF) from platelets: significance for VEGF in cancer biology. Br J Cancer 77 6 (1998) 956-964
113. Thomas K.A. Vascular endothelial growth factor, a potent and selective angiogenic agent. J Biol Chem 271 2 (1996) 603-606
114. Breier G., Damert A., Plate K.H., et al. Angiogenesis in embryos and ischemic diseases. Thromb Haemost 78 1 (1997) 678-683
115. Olander J.V., Connolly D.T., and DeLarco J.E. Specific binding of vascular permeability factor to endothelial cells. Biochem Biophys Res Commun 175 1 (1991) 68-76
116. Peters K.G., De Vries C., and Williams L.T. Vascular endothelial growth factor receptor expression during embryogenesis and tissue repair suggests a role in endothelial differentiation and blood vessel growth. Proc Natl Acad Sci U S A 90 19 (1993) 8915-8919
117. Yebra M., Parry G.C., Strömblad S., et al. Requirement of receptor-bound urokinase-type plasminogen activator for integrin alphavbeta5-directed cell migration. J Biol Chem 271 46 (1996) 29393-29399
118. Suzuma K., Takagi H., Otani A., et al. Hypoxia and vascular endothelial growth factor stimulate angiogenic integrin expression in bovine retinal microvascular endothelial cells. Invest Opthalmol Vis Sci 39 6 (1998) 1028-1035
119. Senger D.R., Ledbetter S.R., Claffey K.P., et al. Stimulation of endothelial cell migration by vascular permeability factor/vascular endothelial growth factor through cooperative mechanisms involving the alphavbeta3 integrin, osteopontin, and thrombin. Am J Pathol 149 1 (1996) 293-305
120. Pepper M.S., Ferrara N., Orci L., et al. Potent synergism between vascular endothelial growth factor and basic fibroblast growth factor in the induction of angiogenesis in vitro. Biochem Biophys Res Commun 189 2 (1992) 824-831
121. Watanabe Y., Lee S.W., Detmar M., et al. Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) delays and induces escape from senescence in human dermal microvascular endothelial cells. Oncogene 14 17 (1997) 2025-2032
122. Hong Y.K., Lange-Asschenfeldt B., Velasco P., et al. VEGF-A promotes tissue repair-associated lymphatic vessel formation via VEGFR-2 and the alpha1beta1 and alpha2- beta1 integrins. FASEB J 18 10 (2004) 1111-1113
123. Walder C.E., Errett C.J., Bunting S., et al. Vascular endothelial growth factor augments muscle blood flow and function in a rabbit model of chronic hindlimb ischemia. J Cardiovasc Pharmacol 27 1 (1996) 91-98
124. Bauters C., Asahara T., Zheng L.P., et al. Site-specific therapeutic angiogenesis after systemic administration of vascular endothelial growth factor. J Vasc Surg 21 2 (1995) 314-324
125. Bauters C., Asahara T., Zheng L.P., et al. Physiological assessment of augmented vascularity induced by VEGF in ischemic rabbit hindlimb. Am J Phys 267 4 Pt 2 (1994) H1263-H1271
126. Takeshita S., Pu L.Q., Stein L.A., et al. Intramuscular administration of vascular endothelial growth factor induces dose-dependent collateral artery augmentation in a rabbit model of chronic limb ischemia. Circulation 90 5 Pt 2 (1994) II228-II234
127. Takeshita S., Zheng L.P., Brogi E., et al. Therapeutic angiogenesis. A single intraarterial bolus of vascular endothelial growth factor augments revascularization in a rabbit ischemic hind limb model. J Clin Invest 93 2 (1994) 662-670
128. Galiano R.D., Tepper O.M., Pelo C.R., et al. Topical vascular endothelial growth factor accelerates diabetic wound healing through increased angiogenesis and by mobilizing and recruiting bone marrow-derived cells. Am J Pathol 164 6 (2004) 1935-1947
129. Baumgartner I., Pieczek A., Manor O., et al. Constitutive expression of phVEGF165 after intramuscular gene transfer promotes collateral vessel development in patients with critical limb ischemia. Circulation 97 12 (1998) 1114-1123
130. Nagy J.A., Vasile E., Feng D., et al. Vascular permeability factor/vascular endothelial growth factor induces lymphangiogenesis as well as angiogenesis. J Exp Med 196 11 (2002) 1497-1506
131. Carmeliet P.V.E.G.F. gene therapy: stimulating angiogenesis or angioma-genesis?. Nat Med 6 10 (2000) 1102-1103
132. Maes C., Carmeliet P., Moermans K., et al. Impaired angiogenesis and endochondral bone formation in mice lacking the vascular endothelial growth factor isoforms VEGF164 and VEGF188. Mech Dev 111 1-2 (2002) 61-73
133. Slater M., Patava J., Kingham K., et al. Involvement of platelets in stimulating osteogenic activity. J Orthop Res 13 5 (1995) 655-663
134. Lowery G.L., Kulkarni S., and Pennisi A.E. Use of autologous growth factors in lumbar spinal fusion. Bone 25 2 Suppl (1999) 47S-50S
135. Whitman D.H., Berry R.L., and Green D.M. Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg 55 11 (1997) 1294-1299
136. Marx R.E., Carlson E.R., Eichstaedt R.M., et al. Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85 6 (1998) 638-646
137. Kevy S.V., and Jacobson M.S. Comparison of methods for point of care preparation of autologous platelet gel. J Extra Corpor Technol 36 1 (2004) 28-35
138. Margolis D.J., Kantor J., Santanna J., et al. Effectiveness of platelet releasate for the treatment of diabetic neuropathic foot ulcers. Diabetes Care 24 3 (2001) 483-488
139. Crovetti G., Martinelli G., Issi M., et al. Platelet gel for healing cutaneous chronic wounds. Transfus Apheresis Sci 30 2 (2004) 145-151
140. Eppley B.L. Platelet rich plasma: a review of biology and applications in plastic surgery. Plast Reconstr Surg 118 6 (2006) 147e-159e
141. Gonshor A. Technique for producing platelet rich plasma and platelet concentrate: background and process. Int J Periodontics Restorative Dent 22 6 (2002) 547-557
142. Gandhi A., Doumas C., O'Connor J.P., et al. The effects of local platelet rich plasma delivery on diabetic fracture healing. Bone 38 4 (2006) 540-546
143. Man D., Plosker H., and Winland-Brown J.E. The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg 107 1 (2001) 229-237
144. Petrungaro P.S. Using platelet-rich plasma to accelerate soft tissue maturation in esthetic periodontal surgery. Compend Contin Educ Dent 22 9 (2001) 729-732
145. Siebrecht M.A.N., De Rooij P.P., Arm D.M., et al. Platelet concentrate increases bone ingrowth into porous hydroxyapatite. Orthopedics 25 (2002) 169-172
146. Marlovits S., Mousavi M., Gäbler C., et al. A new simplified technique for producing platelet-rich plasma: A short technical note. Eur Spine J 13 Suppl 1 (2004) S102-S106
147. Lozada J.L., Caplanis N., Proussaefs P., et al. Platelet-rich plasma application in sinus graft surgery: Part I. Background and processing techniques. J Oral Implantol 27 1 (2001) 38-42
148. Gandhi A., Bibbo C., Pinzur M., et al. The role of platelet-rich plasma in foot and ankle surgery. Foot Ankle Clin 10 4 (2005) 621-637
149. Landesberg R., Roy M., and Glickman R.S. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J Oral Maxillofac Surg 58 3 (2000) 297-300
150. Efeoglu C., Akçay Y.D., and Ertürk S. A modified method of preparing platelet-rich plasma: an experimental study. J Oral Maxillofac Surg 62 11 (2004) 1403-1407
151. Roukis T.S., Zgonis T., and Tiernan B. Autologous platelet-rich plasma for wound and osseous healing: a review of the literature and commercially available products. Adv Ther 23 2 (2006) 218-237
152. Weibrich G., Kleis W.K., Hitzler W.E., et al. Comparison of the platelet concentrate collection system with the plasma-rich-in-growth-factors kit to produce platelet-rich plasma: a technical report. Int J Oral Maxillofac Implants 20 1 (2005) 118-123
153. Weibrich G., Hansen T., Kleis W., et al. Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration. Bone 34 4 (2004) 665-671
154. Zimmermann R., Arnold D., Strasser E., et al. Sample preparation technique and white cell content influence the detectable levels of growth factors in platelet concentrates. Vox Sang 85 4 (2003) 283-289
155. Anderson N.A., Pamphilon D.H., Tandy N.J., et al. Comparison of platelet-rich plasma collection using the Haemonetics PCS and Baxter Autopheresis C. Vox Sang 60 3 (1991) 155-158
156. Christie R.J., Carrington L., and Alving B. Postoperative bleeding induced by topical bovine thrombin: report of two cases. Surgery 121 6 (1997) 708-710
157. Rapaport S.I., Zivelin A., Minow R.A., et al. Clinical significance of antibodies to bovine and human thrombin and factor V after surgical use of bovine thrombin. Am J Pathol 97 1 (1992) 84-91
158. Zehnder J.L., and Leung L.L. Development of antibodies to thrombin and factor V with recurrent bleeding in a patient exposed to topical bovine thrombin. Blood 76 10 (1990) 2011-2016
159. Fennis J.P., Stoelinga P.J., and Jansen J.A. Mandibular reconstruction: a clinical and radiographic animal study on the use of autogenous scaffolds and platelet-rich plasma. Int J Oral Maxillofac Surg 31 3 (2002) 281-286
160. Arpornmaeklong P., Kochel M., Depprich R., et al. Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study. Int J Oral Maxillofac Implants 33 1 (2004) 60-70
161. Slapnicka J., Fassmann A., Strasak L., et al. Effects of activated and nonactivated platelet-rich plasma on proliferation of human osteoblasts in vitro. J Oral Maxillofac Surg 66 2 (2008) 297-301
162. Lucarelli E., Beccheroni A., Donati D., et al. Platelet-derived growth factors enhance proliferation of human stromal stem cells. Biomaterials 24 18 (2003) 3095-3100
163. Kanno T., Takahashi T., Tsujisawa T., et al. Platelet-rich plasma enhances human osteoblast-like cell proliferation and differentiation. J Oral Maxillofac Surg 63 3 (2005) 362-369
164. Tomoyasu A., Higashio K., Kanomata K., et al. Platelet-rich plasma stimulates osteoblastic differentiation in the presence of BMPs. Biochem Biophys Res Commun 361 1 (2007) 62-67
165. Ranly D.M., Lohmann C.H., Andreacchio D., et al. Platelet-rich plasma inhibits demineralized bone matrix-induced bone formation in nude mice. J Bone Joint Surg 89-A 1 (2007) 139-147
166. Ranly D.M., McMillan J., Keller T., et al. Platelet-derived growth factor inhibits demineralized bone matrix-induced intramuscular cartilage and bone formation. A study of immunocompromised mice. J Bone Joint Surg Am 87-A 9 (2005) 2052-2064
167. Butterfield K.J., Bennett J., Gronowicz G., et al. Effect of platelet-rich plasma with autogenous bone graft for maxillary sinus augmentation in a rabbit model. J Oral Maxillofac Surg 63 3 (2005) 370-376
168. Kim E.S., Park E.J., and Choung P.H. Platelet concentration and its effect on bone formation in calvarial defects: an experimental study in rabbits. J Prosthet Dent 86 4 (2001) 428-433
169. Kasten P., Vogel J., Geiger F., et al. The effect of platelet-rich plasma on healing in critical-size long-bone defects. Biomaterials 29 29 (2008) 3983-3992
170. Kroese-Deutman H.C., Vehof J.W., Spauwen P.H., et al. Orthotopic bone formation in titanium fiber mesh loaded with platelet-rich plasma and placed in segmental defects. Int J Oral Maxillofac Implants 37 6 (2008) 542-549
171. Aghaloo T.L., Moy P.K., and Freymiller E.G. Evaluation of platelet-rich plasma in combination with freeze-dried bone in the rabbit cranium. A pilot study. Clin Oral Implants Res 16 2 (2005) 250-257
172. Aghaloo T.L., Moy P.K., and Freymiller E.G. Evaluation of platelet-rich plasma in combination with anorganic bovine bone in the rabbit cranium: a pilot study. Int J Oral Maxillofac Implants 19 1 (2004) 59-65
173. Aghaloo T.L., Moy P.K., and Freymiller E.G. Investigation of platelet-rich plasma in rabbit cranial defects: A pilot study. J Oral Maxillofac Surg 60 10 (2002) 1176-1181
174. Thorwarth M., Rupprecht S., Falk S., et al. Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (PRP)-an immunohistochemical analysis. Biomaterials 26 15 (2005) 2575-2584
175. Wiltfang J., Kloss F.R., Kessler P., et al. Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. An animal experiment. Clin Oral Implants Res 15 2 (2004) 187-193
176. Schlegel K.A., Donath K., Rupprecht S., et al. De novo bone formation using bovine collagen and platelet-rich plasma. Biomaterials 25 23 (2004) 5387-5393
177. Klongnoi B., Rupprecht S., Kessler P., et al. Lack of beneficial effects of platelet-rich plasma on sinus augmentation using a fluorhydroxyapatite or autogenous bone: an explorative study. J Clin Periodontol 33 7 (2006) 500-509
178. Roldán J.C., Knueppel H., Schmidt C., et al. Single-stage sinus augmentation with cancellous iliac bone and anorganic bovine bone in the presence of platelet-rich plasma in the miniature pig. Clin Oral Implants Res 19 4 (2008) 373-378
179. Mooren R.E., Merkx M.A., Bronkhorst E.M., et al. The effect of platelet-rich plasma on early and late bone healing: an experimental study in goats. Int J Oral Maxillofac Surg 36 7 (2007) 626-631
180. Jensen T.B., Rahbek O., Overgaard S., et al. Platelet rich plasma and fresh frozen bone allograft as enhancement of implant fixation. An experimental study in dogs. J Orthop Res 22 3 (2004) 653-658
181. Jensen T.B., Rahbek O., Overgaard S., et al. No effect of platelet-rich plasma with frozen or processed bone allograft around noncemented implants. Int Orthop 29 2 (2005) 67-72
182. Choi B.H., Im C.J., Huh J.Y., et al. Effect of platelet-rich plasma on bone regeneration in autogenous bone graft. Int J Oral Maxillofac Implants 33 1 (2004) 56-59
183. Sarkar M.R., Augat P., Shefelbine S.J., et al. Bone formation in a long bone defect model using a platelet-rich plasma-loaded collagen scaffold. Biomaterials 27 9 (2006) 1817-1823
184. Robiony M., Polini F., Costa F., et al. Osteogenesis distraction and platelet-rich plasma for bone restoration of the severely atrophic mandible: preliminary results. J Oral Maxillofac Surg 60 6 (2002) 630-635
185. Kassolis J.D., and Reynolds M.A. Evaluation of the adjunctive benefits of platelet-rich plasma in subantral sinus augmentation. J Craniofac Surg 16 2 (2005) 280-287
186. Kassolis J.D., Rosen P.S., and Reynolds M.A. Alveolar ridge and sinus augmentation utilizing platelet-rich plasma in combination with freeze-dried bone allograft: case series. J Periodontol 71 10 (2000) 1654-1661
187. Maiorana C., Sommariva L., Brivio P., et al. Maxillary sinus augmentation with anorganic bovine bone (Bio-Oss) and autologous platelet-rich plasma: preliminary clinical and histologic evaluations. Int J Periodontics Restorative Dent 23 3 (2003) 227-235
188. Rodriguez A., Anastassov G.E., Lee H., et al. Maxillary sinus augmentation with deproteinated bovine bone and platelet rich plasma with simultaneous insertion of endosseous implants. J Oral Maxillofac Surg 61 2 (2003) 157-163
189. Consolo U., Zaffe D., Bertoldi C., et al. Platelet-rich plasma activity on maxillary sinus floor augmentation by autologous bone. Clin Oral Implants Res 18 2 (2007) 252-262
190. Thor A., Franke-Stenport V., Johansson C.B., et al. Early bone formation in human bone grafts treated with platelet-rich plasma: preliminary histomorphometric results. Int J Oral Maxillofac Surg 36 12 (2007) 1164-1171
191. Dawson J., Thorogood M., Marks S.A., et al. The prevalence of foot problems in older women: a cause for concern. J Public Health Med 24 2 (2002) 77-84
192. Schaaf H., Streckbein P., Lendeckel S., et al. Topical use of platelet-rich plasma to influence bone volume in maxillary augmentation: a prospective randomized trial. Vox Sang 94 1 (2008) 64-69
193. Schaaf H., Streckbein P., Lendeckel S., et al. Sinus lift augmentation using autogenous bone grafts and platelet-rich plasma: radiographic results. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106 5 (2008) 673-678
194. Kitoh H., Kitakoji T., Tsuchiya H., et al. Distraction osteogenesis of the lower extremity in patients with achondroplasia/hypochondroplasia treated with transplantation of culture-expanded bone marrow cells and platelet-rich plasma. J Pediatr Orthop 27 6 (2007) 629-634
195. Kitoh H., Kitakoji T., Tsuchiya H., et al. Transplantation of culture expanded bone marrow cells and platelet rich plasma in distraction osteogenesis of the long bones. Bone 40 2 (2007) 522-528
196. Kitoh H., Kitakoji T., Tsuchiya H., et al. Transplantation of marrow-derived mesenchymal stem cells and platelet-rich plasma during distraction osteogenesis-a preliminary result of three cases. Bone 35 4 (2004) 892-898
197. Castro F.P.J. Role of activated growth factors in lumbar spinal fusions. J Spinal Disord Tech 17 5 (2004) 380-384
198. Carreon L.Y., Glassman S.D., Anekstein Y., et al. Platelet gel (AGF) fails to increase fusion rates in instrumented posterolateral fusions. Spine 30 9 (2005) E243-E246
199. Jenis L.G., Banco R.J., and Kwon B. A prospective study of autologous growth factors (AGF) in lumbar interbody. Spine J 6 1 (2006) 14-20
200. Weiner B.K., and Walker M. Efficacy of autologous growth factors in lumbar intertransverse fusions. Spine 28 17 (2003) 1968-1970
201. Mariconda M., Cozzolino F., Cozzolino A., et al. Platelet gel supplementation in long bone nonunions treated by external fixation. J Orthop Trauma 22 5 (2008) 342-345
202. Coetzee J.C., Pomeroy G.C., Watts J.D., et al. The use of autologous concentrated growth factors to promote syndesmosis fusion in the agility total ankle replacement. A preliminary study. Foot Ankle Int 26 10 (2005) 840-846
203. Barrow C.R., and Pomeroy G.C. Enhancement of syndesmotic fusion rates in total ankle arthroplasty with the use of autologous platelet concentrate. Foot Ankle Int 26 6 (2005) 458-461
204. Bibbo C., Bono C.M., and Lin S.S. Union rates using autologous platelet concentrate alone and with bone graft in high-risk foot and ankle surgery patients. J Surg Orthop Adv 14 1 (2005) 17-22
205. Grant W.P., Jerlin E.A., Pietrzak W.S., et al. The utilization of autologous growth factors for the facilitation of fusion in complex neuropathic fractures in the diabetic population. Clin Podiatr Med Surg 22 4 (2005) 561-584
206. Boc S.F., D'Angelantonio A., and Grant S. The triplane Austin bunionectomy: A review and retrospective analysis. J Foot Ankle Surg 30 4 (1991) 375-382
207. Kakudo N., Minakata T., Mitsui T., et al. Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts. Plast Reconstr Surg 122 5 (2008) 1352-1360
208. Graziani F., Ivanovski S., Cei S., et al. The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts. Clin Oral Implants Res 17 2 (2006) 212-219
209. Lundquist R., Dziegiel M.H., and Agren M.S. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin. Wound Repair Regen 16 3 (2008) 356-363
210. Phillips G.D., Stone A.M., Whitehead R.A., et al. Platelet derived wound healing factors (PDWHF) accelerate and augment wound healing angiogenesis in the rat. Vivo 8 2 (1994) 167-171
211. Tanaka R., Ichioka S., Sekiya N., et al. Elastic plasma protein film blended with platelet releasate accelerates healing of diabetic mouse skin wounds. Vox Sang 93 1 (2007) 49-56
212. Ito H., Shimizu A., Miyamoto T., et al. Clinical significance of increased mobility in the sagittal plane in patients with hallux valgus. Foot Ankle Int 20 1 (1999) 29-32
213. Moulin V., Lawny F., Barritault D., et al. Platelet releasate treatment improves skin healing in diabetic rats through endogenous growth factor secretion. Cell Mol Biol 44 6 (1998) 961-971
214. Vermeulen P., Dickens S., and Vranckx J.J. Platelet-rich plasma and keratinocytes enhance healing and deposition of fibronectin in a porcine full thickness wound model. J Plast Reconstr Aesthet Surg 60 4 (2006) S14 (O43)
215. Carter C.A., Jolly D.G., Worden C.E.S., et al. Platelet-rich plasma gel promotes differentiation and regeneration during equine wound healing. Exp Mol Pathol 74 3 (2003) 244-255
216. Chandra R.K., Handorf C., West M., et al. Histologic effects of autologous platelet gel in skin flap healing. Arch Facial Plast Surg 9 4 (2007) 260-263
217. Powell D.M., Chang E., and Farrior E.H. Recovery from deep-plane rhytidectomy following unilateral wound treatment with autologous platelet gel. Arch Facial Plast Surg 3 4 (2001) 245-250
218. Danielsen P., Jørgensen B., Karlsmark T., et al. Effect of topical autologous platelet-rich fibrin versus no intervention on epithelialization of donor sites and meshed split-thickness skin autografts: a randomized clinical trial. Plast Reconstr Surg 122 5 (2008) 1431-1440
219. Hom D.B., Linzie B.M., and Huang T.C. The healing effects of autologous platelet gel on acute human skin wounds. Arch Facial Plast Surg 9 3 (2007) 174-183
220. Kazakos K., Lyras D.N., Verettas D., et al. The use of autologous PRP gel as an aid in the management of acute trauma wounds. Injury 40 8 (2009) 801-805
221. Doucette M.M., Fylling C., and Knighton D.R. Amputation prevention in a high-risk population through comprehensive wound-healing protocol. Arch Phys Med Rehabil 70 10 (1989) 780-785
222. Knighton D.R., Fylling C.P., Fiegel V.D., et al. Amputation prevention in an independently reviewed at-risk diabetic population using a comprehensive wound care protocol. Am J Surg 160 5 (1990) 466-471
223. Glover J.L., Weingarten M.S., Buchbinder D.S., et al. A 4-year outcome-based retrospective study of wound healing and limb salvage in patients with chronic wounds. Adv Wound Care 10 1 (1997) 33-38
224. Ganio C., Tenewitz F.E., Wilson R.C., et al. The treatment of chronic nonhealing wounds using autologous platelet-derived growth factors. J Foot Ankle Surg 32 3 (1993) 263-268
225. Knighton D.R., Ciresi K.F., Fiegel V.D., et al. Classification and treatment of chronic nonhealing wounds. Successful treatment with autologous platelet-derived wound healing factors (PDWHF). Ann Surg 204 3 (1986) 322-330
226. Knighton D.R., Ciresi K., Fiegel V.D., et al. Stimulation of repair in chronic, nonhealing, cutaneous ulcers using platelet-derived wound healing formula. Surg Gynecol Obstet 170 1 (1990) 56-60
227. Atri S.C., Misra J., Bisht D., et al. Use of homologous platelet factors in achieving total healing of recalcitrant skin ulcers. Surgery 108 3 (1990) 508-512
228. Steed D.L., Goslen J.B., Holloway G.A., et al. Randomized prospective double-blind trial in healing chronic diabetic foot ulcers. CT-102 activated platelet supernatant, topical versus placebo. Diabetes Care 15 11 (1992) 1598-1604
229. Driver V.R., Hanft J., Fylling C.P., et al. A prospective, randomized, controlled trial of autologous platelet-rich plasma gel for the treatment of diabetic foot ulcers. Ostomy Wound Manage 52 6 (2006) 68-70 72, 74
230. Holloway G.A., Steed D.L., DeMarco J.M., et al. A randomized controlled dose response trial of activated platelet supernatant, topical CT-102 in chronic, non-healing, diabetic wounds. WOUNDS 5 (1993) 160-168
231. Krupski W.C., Reilly L.M., Perez S., et al. A prospective randomized trial of autologous platelet-derived wound healing factors for treatment of chronic nonhealing wounds: a preliminary report. J Vasc Surg 14 4 (1991) 526-532
232. Akeda K., An H.S., Okuma M., et al. Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis. Osteoarthr Cartil 14 12 (2006) 1272-1280
233. Choi Y.C., Morris G.M., and Sokoloff L. Effect of platelet lysate on growth and sulfated glycosaminoglycan synthesis in articular chondrocyte cultures. Arthritis Rheum 23 2 (1980) 220-224
234. Kaps C., Loch A., Haisch A., et al. Human platelet supernatant promotes proliferation but not differentiation of articular chondrocytes. Med Biol Eng Comput 40 4 (2002) 485-490
235. Gaissmaier C., Fritz J., Krackhardt T., et al. Effect of human platelet supernatant on proliferation and matrix synthesis of human articular chondrocytes in monolayer and three-dimensional alginate cultures. Biomaterials 26 14 (2005) 1953-1960
236. Saito M., Takahashi K.A., Arai Y., et al. The preventative effect of platelet-rich plasma and biodegradable gelatin hydrogel microspheres on experimental osteoarthritis in the rabbit knee. Osteoarthr Cartil 15 Suppl 3 (2007) C232
237. Nakagawa K., Sasho T., Arai M., et al. Effects of autologous platelet-rich plasma on the metabolism of human articular chondrocytes. Osteoarthr Cartil 15 Suppl 2 (2007) B134
238. de Mos M., van der Windt A.E., Jahr H., et al. Can platelet-rich plasma enhance tendon repair? A cell culture study. Am J Sports Med 36 6 (2008) 1171-1178
239. Anitua E., Andía I., Sanchez M., et al. Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture. J Orthop Res 23 2 (2005) 281-286
240. Aspenberg P., and Virchenko O. Platelet concentrate injection improves Achilles tendon repair in rats. Acta Orthop Scand 75 1 (2004) 93-99
241. Virchenko O., and Aspenberg P. How can one platelet injection after tendon injury lead to a stronger tendon after 4 weeks? Interplay between early regeneration and mechanical stimulation. Acta Orthop 77 5 (2006) 806-812
242. Virchenko O., Grenegård M., and Aspenberg P. Independent and additive stimulation of tendon repair by thrombin and platelets. Acta Orthop 77 6 (2006) 960-966
243. Mishra A., and Pavelko T. Treatment of chronic elbow tendinosis with buffered platelet-rich plasma. Am J Sports Med 34 11 (2006) 1774-1778