-
2
-
-
0002818114
-
-
M. B. Sporn and A. B. Roberts, Eds. Springer-Verlag, Heidelberg
-
E. W. Raines, D. F. Bowen-Pope, R. Ross, in Handbook of Experimental Pharmacology. Peptide Growth Factors and Their Receptors, M. B. Sporn and A. B. Roberts, Eds. (Springer-Verlag, Heidelberg, 1990), pp. 173-262.
-
(1990)
Handbook of Experimental Pharmacology. Peptide Growth Factors and Their Receptors
, pp. 173-262
-
-
Raines, E.W.1
Bowen-Pope, D.F.2
Ross, R.3
-
11
-
-
1842269605
-
-
note
-
4 and 1% potassium ferrocyanate in 0.1 M Na cacodylate for 3 hours, followed by 1% tannic acid in water for 30 min. The tissue slices were dehydrated and flat embedded. Ultrathin sections encompassing cortical and subcortical regions were contrasted with uranyl acetate and lead citrate and examined in a Zeiss CEM 902 electron microscope.
-
-
-
-
12
-
-
1842359212
-
-
note
-
lcz heterozygote embryos being either PDGF-B -/-or PDGF-B +/+ were identified by PCR.
-
-
-
-
13
-
-
1842324411
-
-
note
-
4, pH 7.5. Heated probes (see below) were added at a concentration of 3 to 8 μg/ml in hybridization solution, and the sections were incubated overnight. Washes after hybridization were carried out in 1 × SSC, 50 to 55% formamide, and 0.1% Tween 20. The entire process was performed at 65° to 72.5°C (the higher stringency was used for the PDGF-B probe). Production of digoxygenin (DIG)-labeled RNA probes and detection of the probes on sections with an alkaline phosphatase-conjugated antibody were accomplished with DIG-labeled uridine triphosphate, the DIG RNA Labeling Kit, and the DIG Nucleic Acid Detection Kit (Boehringer Mannheim) according to the manufacturer's instructions. PDGF-B sense and antisense probes were generated from a 0.8-kbp mouse cDNA containing the full-length coding sequence cloned in pBS-SK. PDGF-Rβ probes were generated from a 461-bp Sac I fragment cloned in pGEM-2. Figure 2 shows the use of antisense probes on 14-μm thick sections. Photography was done with a Nikon photomicroscope. Unstained sections and Normaski optics were used to allow for good sensitivity and resolution. Controls for the specificity of the in situ hybridization signals included sense probe hybridizations and PDGF-B antisense probe hybridization to PDGF-B -/-embryos. No hybridization signal was obtained in any of these controls (14). PDGF-Rβ expression was studied on 206 sections taken from seven littermate pairs of PDGF-B +/+ and -/-embryos. PDGF-B expression was studied on 108 sections taken from two PDGF-B +/+ and one PDGF-B -/-embryos. Representative examples of these analyses are shown in Figs. 2 and 3.
-
-
-
-
14
-
-
1842321511
-
-
P. Lindahl, B. R. Johansson, P. Levéen, C. Betsholtz, data not shown
-
P. Lindahl, B. R. Johansson, P. Levéen, C. Betsholtz, data not shown.
-
-
-
-
15
-
-
0028867704
-
-
M. C. Puri, J. Rossant, K. Alitalo, A. Bernstein, J. Partanen, EMBO J. 14, 5884 (1995).
-
(1995)
EMBO J.
, vol.14
, pp. 5884
-
-
Puri, M.C.1
Rossant, J.2
Alitalo, K.3
Bernstein, A.4
Partanen, J.5
-
17
-
-
9544227474
-
-
P. Carmeliet et al., Nature 383, 73 (1996).
-
(1996)
Nature
, vol.383
, pp. 73
-
-
Carmeliet, P.1
-
18
-
-
0030460424
-
-
S. Davis et al., Cell 87, 1161 (1996).
-
(1996)
Cell
, vol.87
, pp. 1161
-
-
Davis, S.1
-
19
-
-
0345348938
-
-
C. Suri et al., ibid., p. 1171.
-
Cell
, pp. 1171
-
-
Suri, C.1
-
23
-
-
0029001244
-
-
T. N. Sato et al., Nature 376, 70 (1995).
-
(1995)
Nature
, vol.376
, pp. 70
-
-
Sato, T.N.1
-
24
-
-
0026695259
-
-
D. J. Dumont, T. P. Yamaguchi, R. A. Conlon, J. Rossant, M. L. Breitman, Oncogene 7, 1471 (1992).
-
(1992)
Oncogene
, vol.7
, pp. 1471
-
-
Dumont, D.J.1
Yamaguchi, T.P.2
Conlon, R.A.3
Rossant, J.4
Breitman, M.L.5
-
29
-
-
0029132968
-
-
J. Korhonen et al., Blood 5, 1828 (1995).
-
(1995)
Blood
, vol.5
, pp. 1828
-
-
Korhonen, J.1
-
31
-
-
0017380560
-
-
S. M. Buzney, R. N. Frank, S. D. Varma, T. Tanishima, K. H. Gabbay, Invest. Ophthalmol. Visual Sci. 16, 392 (1977).
-
(1977)
Invest. Ophthalmol. Visual Sci.
, vol.16
, pp. 392
-
-
Buzney, S.M.1
Frank, R.N.2
Varma, S.D.3
Tanishima, T.4
Gabbay, K.H.5
-
32
-
-
1842263778
-
-
in preparation
-
P. Lindahl, L. Karlsson, P. Levéen, M. Pekny, M. Pekna, C. Betsholtz, in preparation.
-
-
-
Lindahl, P.1
Karlsson, L.2
Levéen, P.3
Pekny, M.4
Pekna, M.5
Betsholtz, C.6
-
34
-
-
15844370315
-
-
H. Boström et al., Cell 85, 863 (1996); D. Henrique et al., Nature 375, 787 (1995).
-
(1996)
Cell
, vol.85
, pp. 863
-
-
Boström, H.1
-
35
-
-
0029062934
-
-
H. Boström et al., Cell 85, 863 (1996); D. Henrique et al., Nature 375, 787 (1995).
-
(1995)
Nature
, vol.375
, pp. 787
-
-
Henrique, D.1
-
36
-
-
0028273897
-
-
E. Shinbrot, K. G. Peters, L. T. Williams, Dev. Dyn. 199, 169 (1994); L. Holmgren, A. Glaser, S. Pfeifer-Ohlsson, R. Ohlsson, Development 113, 749 (1991).
-
(1994)
Dev. Dyn.
, vol.199
, pp. 169
-
-
Shinbrot, E.1
Peters, K.G.2
Williams, L.T.3
-
37
-
-
0025998409
-
-
E. Shinbrot, K. G. Peters, L. T. Williams, Dev. Dyn. 199, 169 (1994); L. Holmgren, A. Glaser, S. Pfeifer-Ohlsson, R. Ohlsson, Development 113, 749 (1991).
-
(1991)
Development
, vol.113
, pp. 749
-
-
Holmgren, L.1
Glaser, A.2
Pfeifer-Ohlsson, S.3
Ohlsson, R.4
-
38
-
-
1842379433
-
-
note
-
lcz mice; M. Mercola, J. Escobedo, P. Soriano, and W. D. Richardson for probes; and the Swedish Cancer Foundation, the Swedish Medical Research Council (MFR), the IngaBritt and Arne Lundberg Foundation, and the Göteborg University for financial support.
-
-
-
|