Effect of a conservative mutation of an active site residue involved in substrate binding on the hydride tunneling reaction catalyzed by choline oxidase

Archives of Biochemistry and Biophysics

Volumn 489, Issue 1-2, 2009, Pages 10-14

  Quaye, Osbourne ^a   Gadda, Giovanni ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

Author keywords

Choline oxidase; Flavoprotein; Flavoprotein oxidase; Hydride transfer; Hydride tunneling; Kinetic isotope effects; Temperature effects

Indexed keywords

AMINO ACID; ASPARTIC ACID; CHOLINE OXIDASE; GLUTAMIC ACID; ISOTOPE; MUTANT PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; GENETIC CONSERVATION; ION TRANSPORT; NONHUMAN; PHASE TRANSITION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SPECTROPHOTOMETRY; TEMPERATURE DEPENDENCE; THERMODYNAMICS; WILD TYPE;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SUBSTITUTION; ANIMALS; CATALYTIC DOMAIN; HUMANS; MUTAGENESIS, SITE-DIRECTED; MUTATION; POINT MUTATION; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

EID: 69849104100     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2009.07.021     Document Type: Article

References (27)

1. Fan F., and Gadda G. Biochemistry 46 (2007) 6402-6408
2. Finnegan S., and Gadda G. Biochemistry 47 (2008) 13850-13861
3. Gadda G. Biochemistry 47 (2008) 13745-13753
4. Quaye O., Lountos G.T., Fan F., Orville A.M., and Gadda G. Biochemistry 47 (2008) 243-256
5. Ghanem M., and Gadda G. Biochemistry 44 (2005) 893-904
6. Ghanem M., and Gadda G. Biochemistry 45 (2006) 3437-3447
7. Fan F., and Gadda G. J. Am. Chem. Soc. 127 (2005) 17954-17961
8. Cleland W.W. In: Purich D.L. (Ed). Methods in Enzymology (1980), New York 104-125
9. Lewis Jr. C.A., and Wolfenden R. Biochemistry 16 (1977) 4886-4890
10. Fan F., and Gadda G. J. Am. Chem. Soc. 127 (2005) 2067-2074
11. Gadda G. Biochim. Biophys. Acta 1650 (2003) 4-9
12. Basran J., Harris R.J., Sutcliffe M.J., and Scrutton N.S. J. Biol. Chem. 278 (2003) 43973-43982
13. Basran J., Sutcliffe M.J., and Scrutton N.S. Biochemistry 38 (1999) 3218-3222
14. Jonsson T., Edmondson D.E., and Klinman J.P. Biochemistry 33 (1994) 14871-14878
15. Chowdhury S., and Banerjee R. J. Am. Chem. Soc. 122 (2000) 5417-5418
16. Meyer M.P., Tomchick D.R., and Klinman J.P. Proc. Natl. Acad. Sci. USA 105 (2008) 1146-1151
17. Nagel Z.D., and Klinman J.P. Chem. Rev. 106 (2006) 3095-3118
18. Whittaker M.M., Ballou D.P., and Whittaker J.W. Biochemistry 37 (1998) 8426-8436
19. Sharma S.C., and Klinman J.P. J. Am. Chem. Soc. 130 (2008) 17632-17633
20. Agrawal N., Hong B., Mihai C., and Kohen A. Biochemistry 43 (2004) 1998-2006
21. Basran J., Patel S., Sutcliffe M.J., and Scrutton N.S. J. Biol. Chem. 276 (2001) 6234-6242
22. Francisco W.A., Knapp M.J., Blackburn N.J., and Klinman J.P. J. Am. Chem. Soc. 124 (2002) 8194-8195
23. Knapp M.J., Rickert K., and Klinman J.P. J. Am. Chem. Soc. 124 (2002) 3865-3874
24. Kohen A., Cannio R., Bartolucci S., and Klinman J.P. Nature 399 (1999) 496-499
25. Sikorski R.S., Wang L., Markham K.A., Rajagopalan P.T., Benkovic S.J., and Kohen A. J. Am. Chem. Soc. 126 (2004) 4778-4779
26. Wang L., Tharp S., Selzer T., Benkovic S.J., and Kohen A. Biochemistry 45 (2006) 1383-1392
27. Sutcliffe M.J., Masgrau L., Roujeinikova A., Johannissen L.O., Hothi P., Basran J., Ranaghan K.E., Mulholland A.J., Leys D., and Scrutton N.S. Philos. Trans. R. Soc. Lond. B Biol. Sci. 361 (2006) 1375-1386