Substitution of an active site valine uncovers a kinetically slow equilibrium between competent and incompetent forms of choline oxidase

Biochemistry

Volumn 47, Issue 52, 2008, Pages 13850-13861

  Finnegan, Steffan ^a   Gadda, Giovanni ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ACTIVE SPECIES; ARTHROBACTER GLOBIFORMIS; BIOCHEMICAL DATA; BIOSYNTHETIC PATHWAY; CHOLINE OXIDASE; ECONOMIC INTERESTS; ENZYMATIC OXIDATIONS; FLAVIN-DEPENDENT; GLYCINE BETAINE; HALF-REACTIONS; HUMAN PATHOGENS; HYDRIDE ION TRANSFER; HYDROPHOBIC RESIDUES; PROTON ABSTRACTION; SOLVENT KINETIC ISOTOPE EFFECTS; SOLVENT VISCOSITY; STRESS CONDITION;

GENETIC ENGINEERING; HYDROPHOBICITY; ISOTOPES; OXIDATION; PHOSPHATASES; PROTONS; SYNTHESIS (CHEMICAL);

ENZYMES;

ALANINE; CHOLINE OXIDASE; ISOTOPE; QUERCETIN; THREONINE; VALINE; ALCOHOL DEHYDROGENASE;

ARTICLE; ATOM; CATALYSIS; CHEMICAL STRUCTURE; ENZYME ASSAY; ESCHERICHIA COLI; HYDROPHOBICITY; KINETICS; OXIDATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SPECTROSCOPY; AMINO ACID SUBSTITUTION; CHEMISTRY; ENZYME ACTIVE SITE; GENETICS;

ARTHROBACTER GLOBIFORMIS;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; KINETICS; VALINE;

EID: 58849110962     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi801424p     Document Type: Article

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