Mutation of the active site carboxy-lysine (K70) of OXA-1 β-lactamase results in a deacylation-deficient enzyme

Biochemistry

Volumn 48, Issue 26, 2009, Pages 6136-6145

  Schneider, Kyle D ^a   Bethel, Christopher R ^b   Distler, Anne M ^c   Hujer, Andrea M ^b   Bonomo, Robert A ^b,c   Leonard, David A ^a  

^a GRAND VALLEY STATE UNIVERSITY   (United States)

^b LOUIS STOKES CLEVELAND VA MEDICAL CENTER   (United States)

^c CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; AMINO GROUP; ASPARTATE; CATALYTIC TURNOVER; CLASS-D; DEACYLATION; GENERAL BASE; LACTAMASE; LACTAMASES; MIMETICS; SHORT-CHAIN CARBOXYLIC ACIDS;

AMINES; CARBOXYLIC ACIDS; ENZYMES; FLUORESCENCE; FUNCTIONAL GROUPS; ORGANIC ACIDS; SUBSTRATES;

AMINO ACIDS;

ALANINE; AMPICILLIN; ASPARTIC ACID; BETA LACTAMASE; BOCILLIN FL; CARBAMIC ACID; CARBOXYLIC ACID; GLUTAMIC ACID; LYSINE; NITROCEFIN; OXA 1 BETA LACTAMASE; PENICILLIN G; SERINE; TRYPTOPHAN; UNCLASSIFIED DRUG;

ACYLATION AND DEACYLATION; AMINO ACID SUBSTITUTION; ARTICLE; FLUORESCENCE SPECTROSCOPY; MUTATION; PRIORITY JOURNAL; TURNOVER TIME;

ACIDS, ACYCLIC; AMINO ACID SUBSTITUTION; AMPICILLIN; BETA-LACTAMASES; BINDING, COMPETITIVE; BIOCATALYSIS; BORON COMPOUNDS; CATALYTIC DOMAIN; CEPHALOSPORINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME INHIBITORS; ESCHERICHIA COLI; HYDROLYSIS; KINETICS; LYSINE; PENICILLINS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRIAZINES;

EID: 67650095721     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi900448u     Document Type: Article

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