pKa measurements from nuclear magnetic resonance of tyrosine-150 in class C β-lactamase

Biochemical Journal

Volumn 371, Issue 1, 2003, Pages 175-181

  Kato Toma, Yoko ^a   Iwashita, Takashi ^a   Masuda, Katsuyoshi ^a   Oyama, Yoshiaki ^b   Ishiguro, Masaji ^a  

^a SUNTORY INSTITUTE FOR BIOORGANIC RESEARCH   (Japan)

^b SUNTORY LTD   (Japan)

Author keywords

Active site mimics; Buried surface; Cephalosporinase; Matrix assisted laser desorption ionization time of flight (MALDI TOF); Phenolate formation; Solvent accessibility

Indexed keywords

ENZYMES; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SOLVENTS; TITRATION;

CHEMICAL SHIFTS;

BIOCHEMISTRY;

BETA LACTAMASE; LYSINE; OXYGEN; SOLVENT; TYROSINE DERIVATIVE; PEPTIDE FRAGMENT; TYROSINE;

ARTICLE; CALCULATION; CARBON NUCLEAR MAGNETIC RESONANCE; CITROBACTER FREUNDII; NONHUMAN; PH; PKA; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; PROTON TRANSPORT; TITRIMETRY; WILD TYPE; AMINO ACID SEQUENCE; BINDING SITE; CHEMISTRY; CLASSIFICATION; ENZYMOLOGY; GENETICS; MASS SPECTROMETRY; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POINT MUTATION;

CITROBACTER FREUNDII;

AMINO ACID SEQUENCE; BETA-LACTAMASES; BINDING SITES; CITROBACTER FREUNDII; HYDROGEN-ION CONCENTRATION; LYSINE; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; POINT MUTATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TITRIMETRY; TYROSINE;

EID: 0037391280     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20021447     Document Type: Article

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