Identification of residues critical for catalysis in a class C β-lactamase by combinatorial scanning mutagenesis

Protein Science

Volumn 12, Issue 8, 2003, Pages 1633-1645

  Goldberg, Shalom D ^a   Iannuccilli, William ^b   Nguyen, Tuan ^b   Ju, Jingyue ^b   Cornish, Virginia W ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

^b Havemeyer Hall ^*  (United States)

Author keywords

lactamase; Class C; Enzyme mechanism; High throughput sequencing; Scanning mutagenesis

Indexed keywords

ALANINE; AMINO ACID; AMPICILLIN; ANTIBIOTIC AGENT; ARGININE; ASPARAGINE; ASPARTIC ACID; BETA LACTAM; BETA LACTAMASE; CEFALOTIN; GLUTAMIC ACID; GLUTAMINE; GLYCINE; HISTIDINE; ISOLEUCINE; LEUCINE; LYSINE; METHIONINE; PEPTIDE LIBRARY; PHENYLALANINE; PROLINE; SERINE; THREONINE; TRYPTOPHAN; TYROSINE; VALINE; WATER;

ANTIBIOTIC RESISTANCE; ARTICLE; BINDING SITE; CAPILLARY ELECTROPHORESIS; CATALYSIS; CATALYST; CHEMICAL STRUCTURE; CHEMISTRY; CIRCULAR DICHROISM; CLASSIFICATION; COMBINATORIAL SCANNING MUTAGENESIS; DNA SEQUENCE; ENTEROBACTER CLOACAE; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME STRUCTURE; ESCHERICHIA COLI; GENETICS; HYDROGEN BOND; HYDROLYSIS; KINETICS; METABOLISM; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

BASE SEQUENCE; BETA-LACTAMASES; BINDING SITES; CATALYSIS; DRUG RESISTANCE, BACTERIAL; ESCHERICHIA COLI; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PEPTIDE LIBRARY; PROTEIN CONFORMATION;

ENTEROBACTER; ENTEROBACTER CLOACAE; ESCHERICHIA COLI;

EID: 0041842502     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.0302903     Document Type: Article

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