Radical phosphate transfer mechanism for the thiamin diphosphate- and FAD-dependent pyruvate oxidase from Lactobacillus plantarum. Kinetic coupling of intercofactor electron transfer with phosphate transfer to acetyl-thiamin diphosphate via a transient FAD semiquinone/hydroxyethyl-ThDP radical pair

Biochemistry

Volumn 44, Issue 40, 2005, Pages 13291-13303

  Tittmann, Kai ^a   Wille, Georg ^a   Golbik, Ralph ^a   Weidner, Annett ^a   Ghisla, Sandro ^b   Hübner, Gerhard ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON DIOXIDE; CATALYSIS; HYDROGEN PEROXIDE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; OXYGEN; PHOSPHATES;

FLAVIN ADENINE DINUCLEOTIDE (FAD); LACTOBACILLUS PLANTARUM; MARCUS ANALYSIS; THIAMIN DIPHOSPHATE (THDP);

ENZYME KINETICS;

ACETYLTHIAMINE DIPHOSPHATE; CARBON DIOXIDE; COCARBOXYLASE; ENAMINE; FLAVINE ADENINE NUCLEOTIDE; FLAVINE ADENINE NUCLEOTIDE SEMIQUINONE; HYDROGEN PEROXIDE; OXYGEN; PHOSPHATE; PYRUVATE OXIDASE; PYRUVIC ACID; SEMIQUINONE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CATALYSIS; CHEMICAL REACTION KINETICS; DECARBOXYLATION; ELECTRON TRANSPORT; FLUORESCENCE RESONANCE ENERGY TRANSFER; LACTOBACILLUS PLANTARUM; NONHUMAN; OXIDATION REDUCTION POTENTIAL; PHOSPHATE TRANSPORT; PRIORITY JOURNAL; STEADY STATE; TEMPERATURE DEPENDENCE;

CATALYSIS; ELECTRONS; FLAVIN-ADENINE DINUCLEOTIDE; FLAVINS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FREE RADICALS; HYDROGEN-ION CONCENTRATION; KINETICS; LACTOBACILLUS PLANTARUM; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, CHEMICAL; MODELS, STATISTICAL; OXIDATION-REDUCTION; OXYGEN; PHOSPHATES; PYRUVATE OXIDASE; PYRUVIC ACID; SOLVENTS; SPECTROPHOTOMETRY; TEMPERATURE; THERMODYNAMICS; THIAMINE PYROPHOSPHATE; TIME FACTORS;

LACTOBACILLUS PLANTARUM;

EID: 26444617555     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051058z     Document Type: Article

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