Stem cells in microfluidics

Biotechnology Progress

Volumn 25, Issue 1, 2009, Pages 52-60

  Van Noort, Danny ^a   Ong, Siew Min ^a,b   Zhang, Chi ^a,b   Zhang, Shufang ^a,b   Arooz, Talha ^a   Yu, Hanry ^a,b,c  

^a INSTITUTE OF BIOENGINEERING AND NANOTECHNOLOGY   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

3D cell constructs; Microfluidic platforms; Microtechnology; Stem cell

Indexed keywords

3D CELL CONSTRUCTS; CELL FATES; CELL FUNCTIONS; CELL RESEARCHES; FLUORESCENCE MARKERS; MICRO-ENVIRONMENTS; MICRO-FLUIDIC DEVICES; MICROFLUIDIC PLATFORMS; MICROTECHNOLOGY; REAL TIME; STEM CELL; STEM CELL RESEARCHES;

CELL MEMBRANES; FLUIDIC DEVICES; MICROFLUIDICS;

CELL CULTURE;

ANIMAL; ARTICLE; CYTOLOGY; HUMAN; INSTRUMENTATION; METHODOLOGY; MICROFLUIDIC ANALYSIS; MICROFLUIDICS; STEM CELL; THEORETICAL MODEL;

ANIMALS; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES; MICROFLUIDICS; MODELS, THEORETICAL; STEM CELLS;

EID: 64549112273     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.171     Document Type: Article

References (126)

1. Abbott A. Cell culture: biology's new dimension. Nature 2003;424:870-872.
2. Albrecht DR, Underhill GH, Wassermann TB, Sah RL, Bhatia SN. Probing the role of multicellular organization in three-dimensional microenvironments. Nat Methods. 2006;3:369-375.
3. Allen JW, Bhatia SN. Engineering liver therapies for the future. Tissue Eng. 2002;8:725-737.
4. Aguiari P, Leo S, Zavan B, Vindigni V, Rimessi A, Bianchi K, Franzin C, Cortivo R, Rossato M, Vettor R, Abatangelo G, Poz-zan T, Pinton T, Rizzuto R. High glucose induces adipogenic differentiation of muscle-derived stem cells. Proc Natl Acad Sci USA. 2008;105:1226-1231.
5. Amoh Y, Li L, Katsuoka K, Penman S, Hoffman RM. Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons. Proc Natl Acad Sci USA. 2005;102:5503-5534.
6. Anderson JR, Chiu DT, Jackman RJ, Cherniavskaya O, McDonald JC, Wu H, Whitesides SH, Whitesides GM. Fabrication of topo-logically complex three-dimensional microfluidic systems in PDMS by rapid prototyping. Anal Chem. 2000;72:3158-3164.
7. Angele P, Yoo JU, Smith C, Mansour J, Jepsen KJ, Nerlich M Johnstone B. Cyclic hydrostatic pressure enhances the chondro-genic phenotype of human mesenchymal progenitor cells differentiated in vitro. J Orthop Res. 2003;21:451-457.
8. Banks D. Microengineering, MEMS, and Interfacing-a Practical Guide. Boca Raton, FL: CRC Press, Tayler & Francis Group, LLC; 2006.
9. Bao N, Jagadeesan B, Bhunia AK, Yao Y, Lu C. Quantification of bacterial cells based on autofluorescence on a microfluidic platform. J Chromatogr A. 2008;15:153-158.
10. Becker H, Heim U. Hot embossing as a method for the fabrication of polymer high aspect ratio structures. Sens. Actuators A. 2000;83:130-135.
11. Bendall SC, Stewart MH, Menendez P, George D, Vijayaragavan K, Werbowetski-Ogilvie T, Ramos-Mejia V, Rouleau A, Yang J, Bossé M, Lajoie G, Bhatia M. IGF and FGF cooperatively establish the regulatory stem cell niche of pluripotent human cells in vitro. Nature 2007;448:1015-1021.
12. Berthier E, Beebe DJ. Flow rate analysis of a surface tension driven passive micropump. Lab Chip. 2007;7:1475-1478.
13. Bertsch A, Lorenz H, Renaud P. 3D microfabrication by combining microstereolithography and thick resist UV lithography. Sens. Actuators A. 1999;73:14-23.
14. Bhadriraju K, Chen CS. Engineering cellular microenvironments to improve cell-based drug testing. Drug Discov Today. 2002;7:612-620.
15. Bhatia SN, Balis UJ, Yarmush ML, Toner M. Microfabrication of hepatocyte/fibroblast co-cultures: role of homotypic cell interactions. Biotechnol Prog. 1998;14:378-387.
16. Bhatia SN, Balis UJ, Yarmush ML, Toner M. Effect of cell-cell interactions in preservation of cellular phenotype: cocultivation of hepa-tocytes and nonparenchymal cells. Faseb J. 1999;13:1883-1900.
17. Bhatia SN, Yarmush ML, Toner M. Controlling cell interactions by micropatterning in co-cultures: hepatocytes and 3T3 fibroblasts. J Biomed Mater Res. 1997;34:189-199.
18. Blow N. Stem cells: in search of common ground. Nature 2008;451:855-858. Boettcher M, Jaeger M, Kirschbaum M, Mueller T, Schnelle T, Duschl C. Gravitation-driven stress-reduced cell handling. Anal Bioanal Chem. 2008;390:857-863.
19. Borenstein JT, Terai H, King KR, Weinberg EJ, Kaazempur-Mofrad MR, Vacanti JP. Microfabrication technology for vascularized tissue engineering. Biomed Microdevices. 2002;4:167-175.
20. Breslauer DN, Lee PJ, Lee LP. Microfluidics-based systems biology. Mol BioSyst. 2006;2:97-112.
21. Burns MA, Johnson BN, Brahmasandra SN, Handique K, Webster JR, Krishnan M, Sammarco TS, Man PM, Jones D, Heldsinger D, Mastrangelo CH, Burke DT. An integrated nanoliter DNA analysis device. Science 1998;282:484-187.
22. Chen CS, Jiang X, Whitesides GM. Microengineering the environment of mammalian cels in culture. MRS Bull. 2005;30:194-201.
23. Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE. Micro-patterned surfaces for control of cell shape, position, and function. Biotechnol Prog. 1998;14:356-363.
24. Chen Y, Zhong J.F. Microfluidic devices for high-throughput gene expression profiling of single hESC-derived neural stem cells. Methods Mol Biol. 2008;438:293-303. Chia SM, Leong KW, Li J, Xu X, Zeng K, Er PN, Gao SJ, Yu H. Hepatocyte encapsulation for enhanced cellular functions. Tissue Eng. 2000;6:481-495.
25. Chin VI, Taupin P, Sanga S, Scheel J, Gage FH, Bhatia SN. Micro-fabricated platform for studying stem cell fates. Biotechnol Bio-eng. 2004;88:399-415.
26. Chou CF, Austin RH, Bakajin O, Tegenfeldt JO, Castelino JA, Chan SS, Cox EC, Craighead HG, Darnton N, Duke T, Han J, Turner S. Sorting biomolecules with microdevices. Electrophore-sis 2000;21:81-90.
27. Chou CF, Bakajin O, Turner SW, Duke TA, Chan SS, Cox EC, Craighead, HG, Austin RH. Sorting by diffusion: an asymmetric obstacle course for continuous molecular separation. Proc Natl Acad Sci USA 1999a;96:13762-13765.
28. Chou HP, Spence C, Schere A, Quake S. A microfabricated device for sizing and sorting DNA molecules. Proc Natl Acad Sci USA 1999b;96:11-13.
29. Chung BG, Flanagan LA, Rhee SW, Schwartz PH, Lee AP, Monuki ES, Jeon NL. Human neural stem cell growth and differentiation in a gradient-generating microfluidic device. Lab Chip. 2005;5: 401-406.
30. Clayton J. Go with the microflow. Nat Methods. 2005;2:621-627. Cooney CG, Towe BC, Eyster CR. Optical pH, oxygen and carbon dioxide monitoring using a microdialysis approach. Sens Actuators B 2000;69:183-188.
31. Davey RE, Zandstra PW. Spatial organization of embryonic stem cell responsiveness to Autocrine Gp130 Ligands reveals an autoregulatory stem cell niche. Stem Cells 2006;24:2538-2548.
32. Davidsson R, Johansson B, Passoth V, Bengtsson M, Laurell T, Emneus J. Microfluidic biosensing systems. Part II. Monitoring the dynamic production of glucose and ethanol from microchip-immobilised yeast cells using enzymatic chemiluminescent micro-biosensors. Lab Chip. 2004;5:488-494.
33. Debuisson D, Treizebre A, Houssin T, Leclerc E, Bartès-Biesel D, Legrand D, Mazurier J, Arscott S, Bocquest B, Senez V. Nano-scale devices for online dielectric spectroscopy of biological cells. Physiol. Meas. 2008;29:S213-S225.
34. Delamarche E, Bernard A, Schmid H, Michel B, Biebuyck H. Patterned delivery of immunoglobulins to surfaces using microfluidic networks. Science 1997;276:779-781.
35. Effenhauser CS, Bruin GJ, Paulus A. Integrated chip-based capillary electrophoresis. Electrophoresis 1997;18:2203-2213.
36. Eisenstein M. Cell sorting: divide and conquer. Nature 2006; 441:1179-1185.
37. Flaim CJ, Teng D, Chien S, Bhatia SN. Combinatorial signaling microenvironments for studying stem cell fate. Stem Cells Dev. 2008;17:23-39.
38. Folch A, Ayon A, Hurtado O, Schmidt MA, Toner M. Molding of deep polydimethylsiloxane microstructures for microfluidics and biological applications. J Biomech Eng. 1999;121:28-34.
39. Folch A, Jo BH, Hurtado O, Beebe DJ, Toner M. Microfabricated elastomeric stencils for micropatterning cell cultures. J Biomed Mater Res. 2000;52:346-353.
40. Folch A, Toner M. Cellular micropatterns on biocompatible materials. Biotechnol Prog. 1998;14:388-392.
41. Gage FH. Mammalian neural stem cells. Science 2000;287:1433-1438.
42. Ghosh M, Alves C, Tong Z, Tettey K, Konstantopoulos K, Stebe KJ. Multifunctional surfaces with discrete functionalized regions for biological applications. Langmuir 2008;24:8134-8142.
43. Giselbrecht S, Gietzelt T, Gottwald E., Trautmann C., Truckenmül-ler R., Weibezahn K.F. and Welle A. 3D tissue culture substrates produced by microthermoforming of pre-processed polymer films. Biomed Microdevices. 2006;8:191-199.
44. Gopalan SM, Flaim C, Bhatia SN, Hoshijima M, Knoell R, Chien KR, Omens JH, McCulloch AD. Anisotropic stretch-induced hypertrophy in neonatal ventricular myocytes micropatterned on deformable elastomers. Biotechnol Bioeng. 2003;81:578-587.
45. Gray BL, Lieu DK, Collins SD, Smith RL, Barakat AI. Microchannel platform for the study of endothelial cell shape and function. Biomed Microdevices. 2002;4:9-16.
46. Gu W, Zhu X, Futai N, Cho BS, Takayama S. Computerized micro-fluidic cell culture using elastomeric channels and Braille displays. Proc Natl Acad Sci USA. 2004;101:15861-1586.
47. Gunawan RC, Silvestre J, Gaskins HR, Kenis PJA, Leckband DE. Cell migration and polarity on microfabricated gradients of extracellular matrix proteins. Langmuir 2006;22:4250-4258.
48. Hallow DN, Seeger RA, Kamaev PP, Prado GR, LaPlaca MC, Prausnitz MR. Shear-induced intracellular loading of cells with molecules by controlled microfluidics. Biotechnol Bioeng. 2006;99:846-854.
49. Hong JW, Studer V, Hang G, Anderson WF, Quake SR. A nanoli-ter-scale nucleic acid processor with parallel architecture. Nat Biotech. 2004;22:435-439.
50. Horie N, So K, Moriya T, Kitagawa N, Tsutsumi K, Nagata I, Shi-nohara K. Effects of oxygen concentration on the proliferation and differentiation of mouse neural stem cells in vitro. Cell Mol Neurobiol. 2008;28:833-845.
51. http://www.memsinvestorjournal.com/2006/10/stem-cells-and-.html. Huh D, Bahng JH, Ling Y, Wei H-H, Kripfgans OD, Fowlkes JB, Grotberg JB, Takayama S. Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification. Anal Chem. 2007;79:1369-1376.
52. Hui EE, Bhatia SN. Microscale control of cell contact and spacing via three-component surface patterning. Langmuir 2007;23:4103-4107.
53. Hung PJ, Lee PJ, Sabounchi P, Lin R, Lee LP. Continuous perfu-sion microfluidic cell culture array for high-throughput cell-based assays. Biotechnol Bioeng. 2005;89:1-8.
54. Imreh MP, Gertow K, Cedervall J, Unger C, Holmberg K, Szoke K, Csoregh L, Fried G, Dilber S, Blennow E, Ahrlund-Richter L. In vitro culture conditions favoring selection of chromosomal abnormalities in human ES cells. J Cell Biochem. 2006;99:508-516.
55. Iwata H, Akamatsu S, Minami N, Yamada M. Effects of antioxi-dants on the development of bovine IVM/IVF embryos in various concentrations of glucose. Theriogenology 1998;50:365-375.
56. Jang K, Sato K, Igawa K, Chung U-I, Kitamori T. Development of an osteoblast-based 3D continuous-perfuion microfluidic system for drug screening. Anal Bioanal Chem. 2008;390:825-832.
57. Jeon NL, Baskaran H, Dertinger SKW, Whitesides GM, Van de Water L, Toner M. Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated device. Nat Biotechnol. 2002;20:826-830.
58. Jiang X, Bruzewicz DA, Wong AP, Piel M, Whitesides GM. Directing cell migration with asymmetric micropatterns. Proc Natl Acad Sci USA. 2005;102:975-978.
59. Kane BJ, Zinner MJ, Yarmush ML, Toner M. Liver-specific functional studies in a microfluidic array of primary mammalian hepa-tocytes. Anal Chem. 2006;78:4291-4298.
60. Kanichai M, Ferguson D, Prendergast PJ, Campbell VA. Hypoxia promotes chondrogenesis in rat mesenchymal stem cells: a role for AKT and hypoxia-inducible factor (HIF)-1alpha. J Cell Physiol. 2008;216:708-715.
61. Katakoa D, Troian S. Patterning liquid flow on the microscopic scale. Nature 1999;402:794-797.
62. Keenan TM, Folch A. Biomolecular gradients in cell culture systems. Lab Chip. 2008;8:34-57.
63. Khademhosseini A, Langer R, Borenstein J, Vacanti JP. Microscale technologies for tissue engineering and biology. Proc Natl Acad Sci USA. 2006;103:103-108.
64. Khatani SR, Bhatia SN. Microscale culture of human liver cells for drug development. Nat Biotechnol. 2008;26:120-126.
65. Kim B, Kim I, Choi W, Kim SW, Kim J, Lim G. Fabrication of cell-encapsulated alginate microfiber scaffold using microfluidic channel. J Manuf Sci Eng. 2008;130:021016-1-6.
66. Kim L, Vahey MD, Lee HY, Voldman J. Microfluidic arrays for logarithmically perfused embryonic stem cell culture. Lab Chip. 2006;6:394-406.
67. Kwanchanok V, Shuler ML. Incorporation of 3T3-L1 cells to mimic bioaccumulation in a microscale cell culture analog device for toxicity studies. Biotech. Prog. 2004;20:590-597.
68. Leclerc E, David B, Griscom L, Lepioufle B, Fujii T, Layrolle P, Legallaisa C. Study of osteoblastic cells in a microfluidic environment. Biomaterials 2006;27:586-595.
69. Lee PJ, Hung PJ, Lee LP. An artificial liver sinusoid with a microfluidic endothelial-like barrier for primary hepatocyte culture. Biotechnol Bioeng. 2007;97:1340-1346.
70. Li MT, Wang J, Zhuang L, Chou SY. Fabrication of circular optical structures with a 20 nm minimum feature size using nanoimprint lithography. Appl Phys Lett. 2000;77:673-675.
71. Lu L, Langer R, Borenstein J, Vacanti JP. Retinal pigment epithelial cell function on substrates with chemically micropatterned surfaces. Biomaterials 1999;20:2351-2361.
72. Lucchetta EM, Lee JH, Fu LA, Patel NH, Ismagilov RF. Dynamics of Drosophila embryonic patterning network perturbed in space and time using microfluidics. Nature 2005;434:1134-1138.
73. Madou M. Fundamentals of Microfabrication. Boca Raton, FL: CRC Press LLC; 1997.
74. Majeti R, Park CY, Weissman IL. Identification of a hierarchy of multipotent hematopoietic progenitors in human cord blood. Cell Stem Cell 2007;1:635-645.
75. McCormick PM, Nelson RJ, Alonso-Amigo MG, Benvegnu DJ, Hooper HH. Microchannel electrophoretic separations of DNA in injection-molded plastic substrates. Anal Chem. 1997;69:2626-2630.
76. McDonald JC, Duffy DC, Anderson JR, Chiu DT, Wu H, Schueller OJ, Whitesides GM. Fabrication of microfluidic systems in poly (dimethylsiloxane). Electrophoresis 2000;21:27-1-0.
77. Metallo CM, Vodyanik MA, de Pablo JJ, Slukvin II, Palecek SP. The response of human embryonic stem cell-derived endothelial cells to shear stress. Biotechnol. Bioeng. 2008;100:830-837.
78. Moeller HC, Mian MK, Shrivastava S, Chung BG, Khademhosseini A. A microwell array system for stem cell culture. Biomaterials 2008;29:752-763.
79. Mohr JC, de Pablo JJ, Palecek SP. 3-D microwell culture of human embryonic stem cells. Biomaterials 2006;27:6032-6042. Muller R, Schmid P, Munding A, Gronmaier R, Kohn E. Elements for surface microfluidics in diamond. Diamond Relat Mater 2004;13:780-784.
80. Namihira M, Kohyama J, Abematsu M, Nakashima K. Epigenetic mechanisms regulating fate specification of neural stem cells. Philos Trans R Soc Lond B Biol Sci. 2008;363:2099-2109.
81. Ong SM, Chi Zhang C, Toh YC, Kim SH, Foo HL, Tan CH, van Noort D, Park S, Yu H. A gel-free 3D microfluidic cell culture system. Biomaterials 2008;29:3237-3244.
82. Ostrovidov S, Jiang JL, Sakai Y, Fujii T. Membrane-based PDMS microbioreactor for perfused 3D primary rat hepatocyte cultures. Biomed Microdevices. 2004;6:279-287.
83. Palumbo R, Galvez BG, Pusterla T, De Marchis F, Cossu G, Marcu KB, Bianchi ME. Cells migrating to sites of tissue damage in response to the danger signal HMGB1 require NF-kappaB activation. J Cell Biol. 2007;179:33-40.
84. Pampaloni F, Reynaud EG, Ernst HK, Stelzer EHK. Essay: the third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol. 2007;8:839-845.
85. Park SH, Sim WY, Park SW, Yang SS, Choi BH, Park SR, Park K, Min BH. An electromagnetic compressive force by cell exciter stimulates chondrogenic differentiation of bone marrow-derived mesenchymal stem cells. Tissue Eng. 2006;12:3107-3117.
86. Park TH, Shuler ML. Integration of cell culture and microfabrica-tion technology. Biotechnol Prog. 2003;19:243-253.
87. Peerani R, Rao BM, Bauwens C, Yin T, Wood GA, Nagy A, Kuma-cheva E, Zandstra PW. Niche-mediated control of human embryonic stem cell self-renewal and differentiation. EMBO J. 2007;26:4744-4755.
88. Ponte AL, Marais E, Gallay N, Langonné A, Delorme B, Hérault O, Charbord P, Domenech J. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of che-mokine and growth factor chemotactic activities. Stem Cells 2007;25:1737-1745.
89. Quake SR, Schere A. From micro-to nanofabrication with soft materials. Science 2000;290:1536-1540.
90. Ramalho-Santos M, Willenbring H. On the origin of the term ''stem cell''. Cell Stem Cell 2007;1:35-38.
91. Randall GC, Doyle PS. Permeation-driven flow in poly(dimethylsi-loxane) microfluidic devices. PNAS 2005;102:10813-10818.
92. Saha S, Ji L, de Pablo JJ, Palecek SP. Inhibition of human embryonic stem cell differentiation by mechanical strain. J Cell Physiol. 2006;206:126-137.
93. Schmeichel KL, Bissell MJ. Modeling tissue-specific signaling and organ function in three dimensions. J Cell Sci. 2003;116:2377-2388.
94. Senoo H, Tsukada Y, Sato T, Hata R. Co-culture of fibroblasts and hepatic parenchymal cells induces metabolic changes and formation of a three-dimensional structure. Cell Biol Int Rep.1989;2:197-206.
95. Sim WY, Park SW, Park SH, Min BH, Park SR, Yang SS. A pneumatic micro cell chip for the differentiation of human mesenchy-mal stem cells under mechanical stimulation. Lab Chip. 2007;7:1775-1782.
96. Sin A, Chin KC, Jamil MF, Kostove Y, Rao G, Shuler M. The design and fabrication of three-chamber microscale cell culture analog devices with integrated dissolved oxugen sensors. Biotechnol Prog. 2004;20:338-345.
97. Sivaraman A, Leach JK, Townsend S, Iida T, Hogan BJ, Stolz DB, Fry R, Samson LD, Tannenbaum SR, Griffith LG. A microscale in vitro physiological model of the liver: predictive screens for drug metabolism and enzyme induction. Curr Drug Metab. 2005;6:569-591.
98. Stroock AD, Dertiner SKW, Ajdari A, Mezic I, Stone HA, White-sides GM. Chaotic mixer for microchannels. Science 2002;295: 647-651.
99. Tabata T, Takei Y. Morphogens, their identification and regulation. Development 2004;131:703-712.
100. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007;131:861-872.
101. Takayama S, McDonald JC, Ostuni E, Liang MN, Kenis PJ, Ismagi-lov RF, Whitesides GM. Patterning cells and their environments using multiple laminar fluid flows in capillary networks. Proc Natl Acad Sci USA. 1999;96:5545-5548.
102. Takayama S, Ostuni E, LeDuc P, Naruse K, Ingber DE, Whitesides GM. Laminar flows: subcellular positioning of small molecules. Nature 2001;411:1016.
103. Tan W, Desai TA. Microfluidic patterning of cells in extracellular matrix biopolymers: effects of channel size, cell type, and matrix composition on pattern integrity. Tissue Eng. 2003;9:255-267.
104. Taylor AM, Blurton-Jones M, Rhee SW, Cribbs DH, Cotman CW, Jeon NL. A microfluidic culture platform for CNS axonal injury, regeneration and transport. Nat Methods. 2005;2:599-605.
105. Toh YC, Zhang C, Zhang J, Khong YM, Chang S, Samper VD, van Noort D, Hutmacher DW, Yu H. A novel 3D mammalian cell perfusion-culture system in microfluidic channels. Lab Chip.2007;7:302-309.
106. Tourovskaia A, Figueroa-Masot X, Folch A. Differentiation-on-a-chip: a microfluidic platform for long-term cell culture studies. Lab Chip. 2005;5:14-19.
107. Underhill GH, Bhatia SN. High-throughput analysis of signals regulating stem cell fate and function. Curr Opin Chem Biol. 2007;11:357-366.
108. Unger MA, Chou HP, Thorsen T, Scherer A, Quake SR. Monolithic microfabricated valves and pumps by multilayer soft lithography. Science 2000;288:113-116.
109. van Noort D, Landweber LF. Towards a re-programmable DNA computer. J Nat Comput. 2005;4:163-175.
110. Voldman J, Gray ML, Schmidt MA. Microfabrication in biology and medicine. Annu Rev Biomed Eng. 1999;1:401-425.
111. Wang GJ, Chen CL, Hsu SH, Chiang YL. Bio-MEMS fabricated artificial capillaries for tissue engineering. Microsyst Technol. 2005;12:120-127.
112. Wang TW, Wu HC, Wang HY, Lin FH, Sun JS. Regulation of adult human mesenchymal stem cells into osteogenic and chondrogenic lineages by different bioreactor systems. J Biomed Mater Res A. 2008 Apr 2 Published Online.
113. Watt FM, Estrach S, Ambler CA. Epidermal Notch signalling: differentiation, cancer and adhesion. Curr Opin Cell Biol. 2008;20:171-179.
114. Weibel DB, Diluzio WR, Whitesides GM. Microfabrication meets microbiology. Nat Rev Microbiol. 2007;5:209-218.
115. Weibel DB, Garsteck P, Whitesides GM. Combining microscience and neurobiology. Curr Opin Neurobiol. 2005;15:560-567.
116. Whitesides GM. The origins and the future of microfluidics. Nature 2006;442:368-373.
117. Wood DK, Braun GB, Fraikin J-L, Swenson LJ, Reich NO, Cleland AN. A feasible approach to all-electronic digital labeling and readout for cell identification. Lab Chip. 2007;7:469-474.
118. Xia N, Hunt TP, Mayers BT, Alsberg E, Whitesides GM, Wester-velt RM, Ingber DE. Combined microfluidic-micromagnetic separation of living cells in continuous flow. Biomed Microdevices.2006;8:299-308.
119. Yang D, Zhang ZJ, Oldenburg M, Ayala M, Zhang SC. Human embryonic stem cell-derived dopaminergic neurons reverse functional deficit in Parkinsonian Rats. Stem Cells 2008;26:55-63.
120. Yin H, Killeen K, Brennen R, Sobek D, Werlich M, van de Goor T. Microfluidic chip for peptide analysis with an integrated HPLC column, sample enrichment column, and nanoelectrospray tip. Anal Chem. 2005;77:527-533.
121. Zandstra PW, Nagy A. Stem cell bioengineering. Annu Rev Biomed Eng. 2001;3:275-305.
122. Zhang MY, Lee PJ, Hung PJ, Johnson T, Lee LP, Mofrad MRK. Microfluidic environment for high density hepatocyte culture. Biomed Microdevices. 2008;10:117-121.
123. Zhang Q, Xu J-J, Chen H-Y. Glucose microfluidic biosensors based on immobilizing glucose oxidase in poly(dimethylsiloxane) elec-trophoretic microchips. J Chromatogr A. 2006;1135:122-126.
124. Zinchenko YS, Coger RN. Engineering micropatterned surfaces for the coculture of hepatocytes and Kupffer cells. J Biomed Mater Res A. 2005;75:242-248.
125. Zinchenko YS, Culberson CR, Coger RN. Contribution of non-parenchymal cells to the performance of micropatterned hepato-cytes. Tissue Eng. 2006a;12:2241-1251.
126. Zinchenko YS, Schrum LW, Clemens M, Coger RN. Hepatocyte and kupffer cells co-cultured on micropatterned surfaces to optimize hepatocyte function. Tissue Eng. 2006b;12:751-761.