Detection and time course of formation of major thiamin diphosphate-bound covalent intermediates derived from a chromophoric substrate analogue on benzoylformate decarboxylase

Biochemistry

Volumn 48, Issue 5, 2009, Pages 981-994

  Chakraborty, Sumit ^a   Nemeria, Natalia S ^a   Balakrishnan, Anand ^a   Brandt, Gabriel S ^c   Kneen, Malea M ^b   Yep, Alejandra ^b   McLeish, Michael J ^b,d   Kenyon, George L ^b   Petsko, Gregory A ^c   Ringe, Dagmar ^c   Jordan, Frank ^a  

^a RUTGERS UNIVERSITY   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

^c BRANDEIS UNIVERSITY   (United States)

^d INDIANA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABSORBANCE; CHROMOPHORIC SUBSTRATES; COVALENT COMPLEXES; DECARBOXYLASE; DECARBOXYLATION REACTIONS; ENAMINE; ENAMINE INTERMEDIATES; HIGH RESOLUTIONS; NON-OXIDATIVE; REACTION PATHWAYS; THIAMIN DIPHOSPHATE; TIME COURSE; TRANSIENT INTERMEDIATES;

ALDEHYDES; CRYSTAL ATOMIC STRUCTURE;

CARBOXYLATION;

2 OXO 4(PYRIDIN 3 YL) 3 BUTENOIC ACID; 3 (PYRIDIN 3 YL)ACRYLALDEHYDE; ALDEHYDE DERIVATIVE; BENZOYLFORMATE DECARBOXYLASE; BUTYRIC ACID; CARBOXYLYASE; ENAMINE; THIAMIN DIPHOSPHATE; THIAMINE; UNCLASSIFIED DRUG; 2 OXO 4 (PYRIDIN 3 YL) 3 BUTENOIC ACID; 2-OXO-4-(PYRIDIN-3-YL)-3-BUTENOIC ACID; BUTYRIC ACID DERIVATIVE; COCARBOXYLASE; FLUORESCENT DYE; PYRIDINE DERIVATIVE;

ARTICLE; CHEMICAL STRUCTURE; CHROMATOPHORE; COMPLEX FORMATION; COVALENT BOND; CRYSTAL STRUCTURE; DECARBOXYLATION; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTON NUCLEAR MAGNETIC RESONANCE; BINDING SITE; CATALYSIS; CHEMISTRY; COMPARATIVE STUDY; ENZYME SPECIFICITY; ENZYMOLOGY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PROTEIN BINDING; PSEUDOMONAS PUTIDA; TIME; X RAY CRYSTALLOGRAPHY;

BINDING SITES; BUTYRIC ACIDS; CARBOXY-LYASES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; DECARBOXYLATION; FLUORESCENT DYES; KINETICS; PROTEIN BINDING; PSEUDOMONAS PUTIDA; PYRIDINES; SUBSTRATE SPECIFICITY; THIAMINE PYROPHOSPHATE; TIME FACTORS;

EID: 61449240107     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi801810h     Document Type: Article

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