Elucidation of the chemistry of enzyme-bound thiamin diphosphate prior to substrate binding: Defining internal equilibria among tautomeric and ionization states

Biochemistry

Volumn 46, Issue 37, 2007, Pages 10739-10744

  Nemeria, Natalia ^a   Korotchkina, Lioubov ^b   McLeish, Michael J ^c   Kenyon, George L ^c   Patel, Mulchand S ^b   Jordan, Frank ^a  

^a RUTGERS UNIVERSITY   (United States)

^b UNIVERSITY AT BUFFALO   (United States)

^c UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACID-BASE CATALYSTS; SUBSTRATE BINDING; THIAMIN DIPHOSPHATES;

CATALYSIS; IONIZATION; PH EFFECTS; PHASE EQUILIBRIA; STABILIZATION;

COENZYMES;

4' AMINOPYRIMIDINIUM; BENZALDEHYDE LYASE; BENZOYLFORMATE DECARBOXYLASE; CARBOXYLYASE; COCARBOXYLASE; LYASE; MULTIENZYME COMPLEX; PYRIMIDINE; PYRUVATE DEHYDROGENASE; PYRUVATE DEHYDROGENASE E1; PYRUVATE OXIDASE; UNCLASSIFIED DRUG;

ACIDITY; ARTICLE; CATALYSIS; CRYSTALLOGRAPHY; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; IONIZATION; NONHUMAN; PKA; PRIORITY JOURNAL; THERMODYNAMICS;

ALDEHYDE-LYASES; CARBOXY-LYASES; CIRCULAR DICHROISM; ENZYMES; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; LACTOBACILLUS PLANTARUM; PSEUDOMONAS FLUORESCENS; PSEUDOMONAS PUTIDA; PYRUVATE DEHYDROGENASE COMPLEX; PYRUVATE OXIDASE; STEREOISOMERISM; SUBSTRATE SPECIFICITY; THIAMINE PYROPHOSPHATE;

EID: 34548671397     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi700838q     Document Type: Article

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