Evidence for dramatic acceleration of a C-H bond ionization rate in thiamin diphosphate enzymes by the protein environment

Biochemistry

Volumn 44, Issue 7, 2005, Pages 2237-2243

  Zhang, Sheng ^a   Zhou, Leon ^a   Nemeria, Natalia ^a   Yan, Yan ^a   Zhang, Zhen ^a   Zou, Yu ^a   Jordan, Frank ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACCELERATION; CATALYSIS; CATALYST ACTIVITY; CHEMICAL BONDS; COMPLEXATION; ENZYME KINETICS; ESCHERICHIA COLI; IONIZATION; PROTEINS; RATE CONSTANTS;

DIPOLAR INTERMEDIATES; STEADY-STATE METHODS; THIAMIN DIPHOSPHATE-DEPENDENT ENZYMES; ZWITTERIONS;

AMINES;

COCARBOXYLASE; ENAMINE; HYDROXYETHYLTHIAMINE DIPHOSPHATE; PYROPHOSPHATE; PYRUVATE DEHYDROGENASE; THIAMINE DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CHEMICAL BOND; CHEMICAL REACTION KINETICS; ENZYME KINETICS; ESCHERICHIA COLI; ION EXCHANGE; IONIZATION; MOLECULAR STABILITY; PRIORITY JOURNAL; STEADY STATE;

ACETYLATION; DEUTERIUM EXCHANGE MEASUREMENT; ENZYME STABILITY; ESCHERICHIA COLI PROTEINS; HYDROGEN BONDING; KINETICS; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; OXIDATION-REDUCTION; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; PROTONS; PYRUVATE DEHYDROGENASE COMPLEX; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; SUBSTRATE SPECIFICITY; THIAMINE PYROPHOSPHATE;

EID: 14044251561     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi047696j     Document Type: Article

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