Dissection of the stepwise mechanism to β-lactam formation and elucidation of a rate-determining conformational change in β-lactam synthetase

Journal of Biological Chemistry

Volumn 284, Issue 1, 2009, Pages 207-217

  Raber, Mary L ^a   Freeman, Michael F ^a   Townsend, Craig A ^a,b  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HEAVY WATER; ISOTOPES; RATE CONSTANTS; SOLVENTS;

CATALYTIC CYCLES; CHEMICAL BARRIERS; CONFORMATIONAL CHANGE; IONIZABLE GROUPS; PROTEIN CONFORMATIONAL CHANGES; SECOND-ORDER RATE CONSTANTS; SOLVENT ISOTOPE EFFECTS; SUBSTRATE BINDING;

CONFORMATIONS;

BETA LACTAM; BETA LACTAMASE INHIBITOR; CLAVULANIC ACID; DEUTERIUM OXIDE; LYSINE; SYNTHETASE;

ADENYLATION; ANIMAL CELL; ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME SUBSTRATE COMPLEX; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IONIZATION; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS; SOLVENT EFFECT; SYNTHESIS; TEMPERATURE; THERMODYNAMICS; VISCOSITY;

EID: 58649114732     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M805390200     Document Type: Article

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