Engineering membrane protein overproduction in Escherichia coli

Protein Science

Volumn 17, Issue 4, 2008, Pages 673-680

  Molina, Daniel Martinez ^a,b   Cornvik, Tobias ^a,b   Eshaghi, Said ^b   Haeggström, Jesper Z ^b   Nordlund, Pär ^b   Sabet, Marina Ignatushchenko ^b  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Colony filtration; In vitro evolution; Membrane protein; Overexpression; Random mutagenesis; Screening

Indexed keywords

MEMBRANE PROTEIN;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; VALIDATION STUDY;

CLONING, MOLECULAR; DETERGENTS; ESCHERICHIA COLI; GENE LIBRARY; HUMANS; MEMBRANE PROTEINS; MUTAGENESIS; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY;

ESCHERICHIA COLI;

EID: 41649116084     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.073242508     Document Type: Article

References (22)

1. Campbell, J.W., Mercer, W.D., Stammers, D.K., Watson, H.C., Wendell, P.L., Hodgson, G., Duee, E., and Muirhead, H. 1971. X-ray-diffraction studies on enzymes in glycolytic pathway. Cold Spring Harb. Symp. Quant. Biol. 36: 165-170.
2. Cornvik, T., Dahlroth, S.L., Magnusdottir, A., Herman, M.D., Knaust, R., Ekberg, M., and Nordlund, P. 2005. Colony filtration blot: A new screening method for soluble protein expression in Escherichia coli. Nat. Methods 2: 507-509.
3. Daley, D.O., Rapp, M., Granseth, E., Melen, K., Drew, D., and von Heijne, G. 2005. Global topology analysis of the Escherichia coli inner membrane proteome. Science 308: 1321-1323.
4. Drew, D., Sjostrand, D., Nilsson, J., Urbig, T., Chin, C.N., de Gier, J.W., and von Heijne, G. 2002. Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis. Proc. Natl. Acad. Sci. 99: 2690-2695.
5. Drew, D., Froderberg, L., Baars, L., and de Gier, J.W.L. 2003. Assembly and overexpression of membrane proteins in Escherichia coli. Biochim. Biophys. Acta Biomembr. 1610: 3-10.
6. Drew, D., Slotboom, D.J., Friso, G., Reda, T., Genevaux, P., Rapp, M., Meindl-Beinker, N.M., Lambert, W., Lerch, M., Daley, D.O., et al. 2005. A scalable, GFP-based pipeline for membrane protein overexpression screening and purification. Protein Sci. 14: 2011-2017.
7. Eshaghi, S., Hedren, M., Nasser, M.I.A., Hammarberg, T., Thornell, A., and Nordlund, P. 2005. An efficient strategy for high-throughput expression screening of recombinant integral membrane proteins. Protein Sci. 14: 676-683.
8. Iwata, S. 2003. Methods and results in crystallization of membrane proteins (ed. S. Iwata), Vol. 4, pp. 283-297. International University Line, La Jolla, CA.
9. Keenan, R.J., Siehl, D.L., Gorton, R., and Castle, L.A. 2005. DNA shuffling as a tool for protein crystallization. Proc. Natl. Acad. Sci. 102: 8887-8892.
10. Knaust, R.K.C. and Nordlund, P. 2001. Screening for soluble expression of recombinant proteins in a 96-well format. Anal. Biochem. 297: 79-85.
11. Krogh, A., Larsson, B., von Heijne, G., and Sonnhammer, E.L.L. 2001. Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes. J. Mol. Biol. 305: 567-580.
12. Laible, P., Scott, H., Henry, L., and Hanson, D. 2004. Towards higher-throughput membrane protein production for structural genomics initiatives. J. Struct. Funct. Genomics 5: 167-172.
13. Loll, P.J. 2003. Membrane protein structural biology: The high-throughput challenge. J. Struct. Biol. 142: 144-153.
14. Moberg, P., Stahl, A., Bhushan, S., Wright, S.J., Eriksson, A., Bruce, B.D., and Glaser, E. 2003. Characterization of a novel zinc metalloprotease involved in degrading targeting peptides in mitochondria and chloroplasts. Plant J. 36: 616-628.
15. Opekarova, M., Robl, I., Grassl, R., and Tanner, W. 1999. Expression of eukaryotic plasma membrane transporter HUP1 from Chlorella kessleri in Escherichia coli. FEMS Microbiol. Lett. 174: 65-72.
16. Pedelacq, J.D., Piltch, E., Liong, E.C., Berendzen, J., Kim, C.Y., Rho, B.S., Park, M.S., Terwilliger, T.C., and Waldo, G.S. 2002. Engineering soluble proteins for structural genomics. Nat. Biotechnol. 20: 927-932.
17. Quick, M. and Wright, E.M. 2002. Employing Escherichia coli to functionally express, purify, and characterize a human transporter. Proc. Natl. Acad. Sci. 99: 8597-8601.
18. Sorensen, H. and Mortensen, K. 2005. Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microb. Cell Fact. 4: 1. doi: 10.1186/1475-2859-4-1.
19. Tobbell, D.A., Middleton, B.J., Raines, S., Needham, M.R.C., Taylor, I.W.F., Beveridge, J.Y., and Abbott, W.M. 2002. Identification of in vitro folding conditions for procathepsin S and cathepsin S using fractional factorial screens. Protein Expr. Purif. 24: 242-254.
20. Tucker, J. and Grisshammer, R. 1996. Purification of a rat neurotensin receptor expressed in Escherichia coli. Biochem. J. 317: 891-899.
21. Waldo, G.S. 2003. Genetic screens and directed evolution for protein solubility. Curr. Opin. Chem. Biol. 7: 33-38.
22. Zhou, Y.F. and Bowie, J.U. 2000. Building a thermostable membrane protein. J. Biol. Chem. 275: 6975-6979.