Improved yield of a ligand-binding GPCR expressed in E. coli for structural studies

Protein Expression and Purification

Volumn 64, Issue 1, 2009, Pages 32-38

  Attrill, Helen ^a   Harding, Peter J ^a   Smith, Eleanor ^a   Ross, Simon ^a   Watts, Anthony ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Autoinduction; G protein coupled receptor; GPCR; Neurotensin; Neurotensin receptor

Indexed keywords

BUFFER; G PROTEIN COUPLED RECEPTOR; HYBRID PROTEIN; LIGAND; NEUROTENSIN; NEUROTENSIN RECEPTOR; NEUROTENSIN TYPE 1 RECEPTOR; PHOSPHOLIPID;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PH; PROTEIN BINDING; RAT; SOLUBILITY; TEMPERATURE;

AMINO ACID SEQUENCE; ANIMALS; BUFFERS; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; LIGANDS; MOLECULAR SEQUENCE DATA; NEUROTENSIN; PHOSPHOLIPIDS; PROTEIN BINDING; RATS; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, NEUROTENSIN; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TEMPERATURE;

ESCHERICHIA COLI; RATTUS;

EID: 57649105479     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.10.001     Document Type: Article

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