Expression of δ, κ and μ human opioid receptors in Escherichia coli and reconstitution of the high-affinity state for agonist with heterotrimeric G proteins

European Journal of Biochemistry

Volumn 260, Issue 2, 1999, Pages 430-438

  Stanasila, Laura ^a   Massotte, Dominique ^a   Kieffer, Brigitte L ^a   Pattus, Franc ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

Expression in Escherichia coli; GTP binding protein; Opioid receptor; Pharmacology; Reconstitution

Indexed keywords

DIPRENORPHINE; ENDORPHIN; GUANINE NUCLEOTIDE BINDING PROTEIN; KAPPA OPIATE RECEPTOR; MALTOSE BINDING PROTEIN; MEMBRANE PROTEIN; MU OPIATE RECEPTOR; OPIATE RECEPTOR; SIGMA OPIATE RECEPTOR;

ARTICLE; BACTERIAL MEMBRANE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CYTOPLASM; DISSOCIATION CONSTANT; ESCHERICHIA COLI; IMMUNOBLOTTING; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING;

ANIMALS; COS CELLS; DIPRENORPHINE; ESCHERICHIA COLI; GTP-BINDING PROTEINS; HUMANS; KINETICS; RECEPTORS, OPIOID, DELTA; RECEPTORS, OPIOID, KAPPA; RECEPTORS, OPIOID, MU; SODIUM;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

EID: 0033105541     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00187.x     Document Type: Article

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