Hot Start PCR with heat-activatable primers: A novel approach for improved PCR performance

Nucleic Acids Research

Volumn 36, Issue 20, 2008, Pages

  Lebedev, Alexandre V ^a   Paul, Natasha ^a   Yee, Joyclyn ^a   Timoshchuk, Victor A ^a   Shum, Jonathan ^a   Miyagi, Kei ^a   Kellum, Jack ^a   Hogrefe, Richard I ^a   Zon, Gerald ^a,b  

^a TriLink BioTechnologies Inc   (United States)

^b APPLIED BIOSYSTEMS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 OXO 1PENTYLOLIGONUCLEOTIDE; DNA POLYMERASE; OLIGONUCLEOTIDE; PHOSPHODIESTER OLIGONUCLEOTIDE; PHOSPHOTRIESTER OLIGONUCLEOTIDE; POLYNUCLEOTIDE; PRIMER DNA; RECOMBINANT DNA; UNCLASSIFIED DRUG; DNA DIRECTED DNA POLYMERASE; FLUORESCENT DYE; ORGANIC COMPOUND; ORGANOPHOSPHORUS COMPOUND; PHOSPHORAMIDOUS ACID; SYBR GREEN I; VIRUS DNA;

ARTICLE; CONTROLLED STUDY; DNA MODIFICATION; DNA PROBE; ENZYME SUBSTRATE; GENE AMPLIFICATION; GENE NUMBER; GENE TECHNOLOGY; HEATING; HIGH TEMPERATURE; INCUBATION TEMPERATURE; METHODOLOGY; NUCLEOTIDE BINDING SITE; NUCLEOTIDE METABOLISM; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; CHEMISTRY; DIMERIZATION; DNA TEMPLATE; EVALUATION; GENETICS; HEAT; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; KINETICS; METABOLISM; POLYMERASE CHAIN REACTION; SYNTHESIS;

DIMERIZATION; DNA PRIMERS; DNA, VIRAL; DNA-DIRECTED DNA POLYMERASE; FLUORESCENT DYES; HIV-1; HOT TEMPERATURE; HUMANS; KINETICS; ORGANIC CHEMICALS; ORGANOPHOSPHORUS COMPOUNDS; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TEMPLATES, GENETIC;

EID: 56649100796     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkn575     Document Type: Article

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