HLA typing of aortic tissues from unidentified bodies using hot start polymerase chain reaction-sequence specific primers

Legal Medicine

Volumn 5, Issue SUPPL. 1, 2003, Pages

  Sato, Yayoi ^a   Hayakawa, Mutsumi ^a   Nakajima, Toru ^a   Motani, Hisako ^a   Kiuchi, Masahiro ^a  

^a CHIBA UNIVERSITY   (Japan)

Author keywords

Aorta; DNA typing; Forensic science; HLA; Hot start PCR; Sequence specific primers

Indexed keywords

CHLOROFORM; DNA; HLA A ANTIGEN; HLA ANTIGEN; HLA B ANTIGEN; HLA DQ ANTIGEN; HLA DR ANTIGEN; PHENOL;

ALLELE; AORTA; AUTOPSY; BONE TISSUE; BURN; CLINICAL ARTICLE; CONFERENCE PAPER; CONTROLLED STUDY; DNA EXTRACTION; DNA FINGERPRINTING; DROWNING; FORENSIC IDENTIFICATION; GENE LOCUS; HLA TYPING; HUMAN; HUMAN TISSUE; POLYMERASE CHAIN REACTION; SAMPLING; TOOTH;

EID: 0038077505     PISSN: 13446223     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1344-6223(02)00108-6     Document Type: Conference Paper

References (6)

1. Olerup O, Zetterquist H. HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: An alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens 1992;39:225-235.
2. Sato Y, Saitoh H, Abe M, Kurosaki K, Kiuchi M. DNA typing for HLA-DR from forensic samples using hot start PCR-SSP method. In: Fisher BAJ, editor. Proceedings of the 15th Meeting of the International Association of Forensic Sciences (IAFS), Los Angeles, CA, Colorado Springs, CO: McCormick-Armstrong, 1999. 1999. pp. 12-13.
3. Ota M, Katsuyama Y, Liu CY, Arakura A, Fukushima H. Validation of HLA-DR locus typing in forensic specimens by combining PCR-SSP with PCR-RFLP. J Forensic Sci 1997;42:929-934.
4. Chen D-F, Pastucha LT, Chen H-Y, Kadar JG, Stangel W. Simultaneous genotyping of human platelet antigens by hot start sequence-specific polymerase chain reaction with DNA polymerase AmpliTaq Gold. Vox Sang 1997;72:192-196.
5. PUREGENE™ DNA Isolation Kit Protocol, Gentra Systems, Minneapolis, MN, 2000.
6. +™ SSP HLA-DQ "Low Resolution" Protocol, DYNAL Biotech Ltd., UK, 2001.