Controlled hot start and improved specificity in carrying out PCR utilizing touch-up and loop incorporated primers (TULIPS)

BioTechniques

Volumn 29, Issue 5, 2000, Pages 1018-1024

  Ailenberg, M ^a   Silverman, M ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED DNA POLYMERASE ALPHA; GELATINASE A; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE;

ARTICLE; CHROMOSOME PAIRING; DNA CONFORMATION; DNA FLANKING REGION; DNA POLYMORPHISM; DNA SEQUENCE; DNA TEMPLATE; GENE AMPLIFICATION; HOUSEKEEPING GENE; POLYMERASE CHAIN REACTION; TRANSGENE; TRANSGENIC MOUSE;

EID: 0033729713     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00295st03     Document Type: Article

References (16)

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2. Site-directed mutagenesis using a PCR-based staggered re-annealing method without restriction enzymes
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4. The elimination of primer-dimer accumulation in PCR
5. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplification
6. Maximizing sensitivity and specificity of PCR by preamplification heating
7. Suppression subtractive hybridization: A versatile method for identifying differentially expressed genes
8. Touchdown PCR to circumvent spurious priming during gene amplification
9. Production of transgenic mice
10. An alternative hot start technique for PCR in small volumes using beads of wax-embedded reaction components dried in trehalose
11. Specificity-enhanced hot-start-PCR: Addition of double-stranded DNA fragments adapted to the annealing temperatures
12. TaqStart antibody: 'Hot start' PCR facilitated by neutralizing monoclonal antibody directed against Taq DNA polymerase
13. Simple method for 'hot starting' RT-PCR
14. Inhibition of multiple thermostable DNA polymerases by a heterodimeric aptamer
15. Enhancement of PCR amplification yield and specificity using AmpliTaq Gold DNA polymerase
16. A hot-start reverse transcription-polymerase chain reaction protocol that initiates multiple analyses simultaneously