Thermally reversible inactivation of Taq polymerase in an organic solvent for application in hot start PCR

Enzyme and Microbial Technology

Volumn 36, Issue 7, 2005, Pages 947-952

  Louwrier, Ariel ^a   Van Der Valk, Anne ^a,b  

^a ABgene House   (United Kingdom)

^b NONE   (United Kingdom)

Author keywords

Organic solvent; PCR; Reversible modification; Taq polymerase

Indexed keywords

CONCENTRATION (PROCESS); ORGANIC SOLVENTS; PH EFFECTS; SOLUTIONS; TEMPERATURE MEASUREMENT;

AMORPHOUS POWDER; DICARBOXYLIC ACID; INCUBATION; REACTIVATION;

ENZYMES;

ACID ANHYDRIDE; CARBOHYDRATE; DICARBOXYLIC ACID DERIVATIVE; DNA POLYMERASE; EXCIPIENT; ORGANIC SOLVENT;

AQUEOUS SOLUTION; ARTICLE; CHEMICAL MODIFICATION; CONCENTRATION (PARAMETERS); ENZYME INACTIVATION; ENZYME STABILITY; INCUBATION TIME; PH; POLYMERASE CHAIN REACTION; POWDER; TEMPERATURE;

EID: 17844389602     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2005.01.019     Document Type: Article

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