Hypocholesteremic and antioxidant effects of garlic (Allium sativum L.) extract in rats fed high cholesterol diet

Pakistan Journal of Nutrition

Volumn 8, Issue 2, 2009, Pages 161-166

  Al Numair, Khalid S ^a  

^a KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

Garlic; Lipid profile; Malondialde; Total antioxidant capacity

Indexed keywords

GARLIC EXTRACT; GLUTATHIONE PEROXIDASE; HIGH DENSITY LIPOPROTEIN CHOLESTEROL; LIPID; LOW DENSITY LIPOPROTEIN CHOLESTEROL; MALONALDEHYDE; SUPEROXIDE DISMUTASE; TRIACYLGLYCEROL;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIOXIDANT ACTIVITY; ARTICLE; CHOLESTEROL BLOOD LEVEL; CHOLESTEROL DIET; CONTROLLED STUDY; DRUG DOSE COMPARISON; DRUG MEGADOSE; ENZYME ACTIVITY; GARLIC; HYPERCHOLESTEROLEMIA; HYPOCHOLESTEROLEMIA; LIPID BLOOD LEVEL; LOW DRUG DOSE; MALE; NONHUMAN; OXIDATIVE STRESS; PROTEIN BLOOD LEVEL; RAT; TISSUE LEVEL; TRIACYLGLYCEROL BLOOD LEVEL;

ALLIUM SATIVUM; RATTUS;

EID: 54949107735     PISSN: 16805194     EISSN: None     Source Type: Journal    
DOI: 10.3923/pjn.2009.161.166     Document Type: Article

References (53)

1. Agarwal, K.C., 1996. Therapeutic action of garlic constituents. Medical Research Review, 16: 111-124.
2. Allain, C.C., L.S. Poon, W. Richmond and P.C. Fu, 1974. Enzymatic determination of total cholesterol in plasma. Clin. Chem., 470-475.
3. Aouadi, R., A. Aouadet, A. Elkadhi, C. Ben Rayana, H. Jaafoura, B. Tritar and K. Nagati, 2000. Effect of fresh garlic (Allium Sativum) on lipid metabolism in male rats. Nutrition Research, 20: 273-280.
4. Arivazhagan, S., S. Balascnthil and S. Nagini, 1999a. Chemopreventive potential of garlic and neem during gastric carcionogensis induced by N-methyl-N′-nitro-N-nitrosoguanidine. Med. Sci. Res., 27: 209-212.
5. Arivazhagan, S., S. Balascnthil and S. Nagini, 1999b. Modulatory effects of garlic and neem leaf extracts on circulatory lipid peroxides and antioxidants during N-methyl-N′-nitro-N-nitrosogunidine-induced gastric carcinogensis. Med. Sci. Res., 27: 527-529.
6. Arivazhagan, S., S. Balascnthil and S. Nagini, 2000. Garlic and neem leaf extracts enhance hepatic glutathione and glutationic-dependent enzymes during N-methyl-N′-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis in rats. Phytotherapy Res., 14: 291-293.
7. Auer, W., A. Eiber, E. Hertkorn, E. Hoehfeld, U. Koehrle, A. Lorenz, F. Mader, W. Merx, G. Otto and B. Schmid-Otto, 1990. Hypertension and hyperlipidemia: garlic helps in mild cases. Br. J. Clin. Pract., 69: 3-6.
8. Augusti, K.T., 1977. Hypocholesterolemic effect of garlic, Allium sativum Linn. Ind. J. Exp. Biol., 15: 489-490.
9. Balascnthil, S., S. Arivazhagan, C.R. Ramachandran and S. Nagini, 1999. Effects of garlic on 7, 12-dimethylbenz [a] anthracene-induced hamster buccal pouch carcinogenesis. Cancer Detection and Prevention, 23: 534-538.
10. Banerjee. S.K., M. Maulik, S.C. Mancahando, A.K. Dinda, S.K. Gupta and S.K. Maulik, 2002. Dose-dependent induction of endogenous antioxidants in rat heart by chronic administration of garlic. Life Sci., 70: 1509-1518.
11. Bordia, A. and H.C. Bansal, 1973. Essential oil of garlic in prevention of atherosclerosis. Lancet, 2: 1491-1492.
12. Bordia, A.K., S.K. Sodhya, A.S. Rathore and N. Bhu, 1975. Essential oil of garlic on blood lipids and fibrinolytic activity in patients of coronary artey disease. J. Asso. Physicians Ind., 26: 327-331.
13. Borek, C., 2001. Antioxidant health effects of aged garlic extract. J. Nutr., 131: 1010S-1015S.
14. Dillon, S.A., R.S. Burmi, G.M. Lowe, D. Billigton and K. Rahman, 2003. Antioxidant properties of aged garlic extract: an in vitro study incorporating human low-density lipoprotein. Life Sci., 72: 1583-1594.
15. Durak, L., M. Kavutcu, B. Aytac, A. Avci, E. Devrim, H. Özbek and S.H. Öxtürk, 2004. Effect of garlic extract consumption on blood lipid and oxidant/antioxidant parameters in humans with high blood cholesterol. J. Nutr. Biochem., 15: 373-377.
16. Elkayam, A., D. Mirelman, E. Peleg, M. Wilcheh, T. Miron, A. Rabinkov, M. Oron-Henman and T. Rosenthal, 2003. The effects of allicin on weight in fructose-induced hyperinsulinemic, hyperlipidemic, hypertensive rats. Am. J. Hypertension, 16: 1053-1056.
17. Folch, M.L. and G.H. Sloane, 1975. Simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem., 226: 497-509.
18. Fossati, P. and L.L. Prencipe, 1982. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem., 28: 2077-2080.
19. Friedewald, W.T., R.I. Levy and D.S. Fredrickson, 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem., 18: 499-502.
20. Gebhardt, R., 1991. Inhibition of cholesterol biosynthesis by a water soluble garlic extract in primary cultures of rat hepatocytes. Arzneimittelforschung, 41: 800-804.
21. Gebhardt, R., 1993. Multiple inhibitory effects of garlic extract on cholesterol biosynthesis in hepatocytes. Lipids, 8: 613-619.
22. Gebhardt, R. and H. Beck, 1996. Differential inhibitory effects of garlic-derived organosulfur compounds on cholesterol biosynthesis in primary rat hepatocyte cultures. Lipids, 1269-1276.
23. Gofman, J.W., W. Young and R. Tandy, 1996. Ischemic heart disease, atherosclerosis and longevity. Circulation, 34: 679-685.
24. Gorinstein, S., H. Leontowicz, M. Leontowicz, J. Drzewiecki, K. Najman, E. Katrrich, D. Garasch, K. Yamamoto and S. Trakhtenberg, 2006. Raw and boiled garlic enhances plasma antioxidant activity and improves plasma lipid metabolism in cholesterol-fed rats. Life Sciences, 78: 655-663.
25. Heid, C.A., J. Stevens, K.J. Livak and P.M. Williams, 1996. Real time quantitative PCR. Genome Research, 6: 986-994.
26. Hino, K, M. Nakamura, K. Nakanishi and M. Monabe, 1996. A new method for the homogenous assay of serum HDL-Cholesterol. Clin. Chem., 42: 299-302.
27. Horie, T., T. Murayama and T. Mishima, 1989. Protection of liver microsomal membranes from lipid peroxidation by garlic extract. Planta. Medica, 55: 506-508.
28. Jain, R.C., 1977. Effect of garlic on serum lipids, coagulability and fibrinolytic activity of blood. Am. J. Clin. Nutr., 30: 13801381.
29. Jain, R.C. and C.R. Vyas, 1975. Garlic and alloxan-induced diabetic rabbits. Am. J. Clin. Nutr., 28: 684-685.
30. Koscielny, J., D. Klüßendorf, R. Latza, R. Schmitt, H. Radtke, G. Siegel and J. Kieswetter, 1999. The antiatherosclerotic effect of Allium sativum. Atherosclerosis, 144: 237-249.
31. Lau, B.H., 2001. Suppression of LDL oxidation by garlic. J. Nutr., 131: 985S-988S.
32. Miller, C., M.J. Rice-Evans, V. Davies, Q.A. Gopinathan and A. Milner, 1993. A novel method for measuring antioxidant capacity and its application to monitoring antioxidant status in premature neonates. Clin. Sci., 84: 407-412.
33. Munday, J.S., K.A. James, L.M. Ray, S.W. Kirkawood and K.G. Thompson, 1999. Daily supplementation with aged garlic, but not raw garlic, protects low density lipoprotein against in vitro oxidation. Atherosclerosis, 1453: 399-404.
34. Ohkawa, H., N. Ohishi and K. Yagi, 1979. Assay for lipid peroxides in animal tissues by thiobarbitiuric acid reaction. Analytical Biochemistry, 95: 351-358.
35. Ou, C.C., S.M. Tsao, M.C. Lin and M.C. Yin, 2003. Protective action on human LDL against oxidation and glycation by four organosulfur compounds derived from garlic. Lipids, 38: 219-224.
36. Pinto, J.T., C. Qiao and J. Zing, 1997. Effects of garlic thioallyl derivatives on growth, glutathione concentration and polyamine formation of human prostate carcinoma cells in culture. Am. J. Clin. Nutr., 66: 398-405.
37. Poglia, D.E. and V.W. Alenline, 1970. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med., 70: 158-178.
38. Prasad, K., V.A. Laxdal, M. Yu and B.L. Raney, 1995. Antioxidant activity of allicin and active principal in garlic. Mol. Cell. Biochem., 148: 183-189.
39. Rahman, K., 2001. Historical perspective on garlic and cardiovascular disease. J. Nutr., 131: 977S-979S.
40. Rassoul, F., V. Richter and W.W. Rotzsch, 1992. Total and HDL-cholesterol screening in the town of Leipzig: influence of diet and Allium sativum. Eur. J. Clin. Res., 3A: 9.
41. Reeves, P.C., F.H. Nielson and G.C. Fahey, 1993. AI-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J. Nutr., 123: 1939-1951.
42. Richter, V., F. Rassoul and W. Rotzsch, 1992. Postprandial hyperlipidaemia under the influence of Allium sativum. Eur. J. Clin. Res., 3A: 10.
43. Santos, O.S. and J. Grunwals, 1993. Effects of garlic powder tablets on blood lipids and blood pressures. A six month placebo-controlled double blind study. Br. J. Clin., 4: 37-44.
44. Silagy, C.A. and H.A. Neil, 1994. A meta-analysis of the effect of garlic on blood pressure. J. Hypertens., 12: 463-468.
45. Slowing, K., P. Ganado, M. Sanz, M. Ruiz and T. Tejerina, 2001. Study of garlic extract and fractions on cholesterol plasma levels and vascular reactivity in cholesterol-fed-rats. J. Nutr., 131: 994S-999S.
46. Sodimu, O., P.K. Joseph and K.T. Augusti, 1984. Certain biochemical effects of garlic oil on rats maintained on high fat- high cholesterol diet. Experiential, 40: 78-80.
47. Steiner, M., A.H. Khan, D. Holbert and R.I. Lin, 1996. A double-blind crossover study in moderately hypercholesterolemic men that compared the effect of aged garlic extract and placebo administration on blood lipids. Am. J. Clin. Nutr., 64: 866-870.
48. Steiner, M. and W. Li, 2001. Aged garlic extract, a modulator of cardiovascular risk factors a dose-finding study on the effects of AGE on platelet functions. J. Nutr., 31: 980S-984S.
49. Thomson, M., K.K. Al-Qattan, T. Bordia and M. Ali, 2006. Including garlic in the diet may help lower blood glucose, cholesterol and triglycerides. J. Nutr., 136: 800S-802S.
50. Walper, A., F. Rassoul, K. Purschwitz and V. Schulz, 1994. Effizienz einer Diätempfehlung and einer zusätzlichen Phytotherapie mit Allium sativum bie leichter bis mäßiger Hypercholesterolämie. Med. Welt., 45: 3-6.
51. Wei, Z. and B.H. Lau, 1998. Garlic inhibits free radical generation and augments antioxidant enzyme activity in vascular endothelial cells. Nutr. Res., 18: 61-70.
52. Wolliams, J.A., G. Wiener, P.H. Anderson and C.H. McMurray, 1983. Variation in the activity of glutathione peroxidase and superoxide dismutase in the blood in various breed crosses of sheep. Research in Veterinary Science, 34: 253-256.
53. Yeh, Y.Y. and S.M. Yeh, 1994. Garlic reduces plasma lipids by inhibiting hepatic cholesterol and triacylglycerol synthesis. Lipids, 29: 189-193.