Chapter 1 Approaches for Studying Angiogenesis-Related Signal Transduction

Methods in Enzymology

Volumn 443, Issue , 2008, Pages 1-23

  Derakhshan, Behrad ^a,b   Harrison, Kenneth D ^a,b   Miao, Qing Robert ^a   Sessa, William C ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; ARGININE; CARBON 14; CITRULLINE; CYCLIC GMP; ENDOTHELIAL NITRIC OXIDE SYNTHASE; NITRIC OXIDE; NITRITE; PROTEIN KINASE B; RAC PROTEIN; RAC1 PROTEIN; RNA; TRITIUM; VASCULOTROPIN; N(G) NITROARGININE METHYL ESTER; VASCULOTROPIN A;

ANGIOGENESIS; BIOASSAY; CATION EXCHANGE; CELL CULTURE; CELL LYSATE; CELL SURVIVAL; CHEMOLUMINESCENCE; ENDOTHELIUM CELL; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME PHOSPHORYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN FUNCTION; REVIEW; RNA INTERFERENCE; SAMPLING; SIGNAL TRANSDUCTION; STRESS FIBER; ANIMAL; ARTICLE; HUMAN; LUMINESCENCE; METABOLISM; NEOVASCULARIZATION (PATHOLOGY); PATHOPHYSIOLOGY; PHYSIOLOGY;

ANIMALS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; LUMINESCENCE; NEOVASCULARIZATION, PATHOLOGIC; NEOVASCULARIZATION, PHYSIOLOGIC; NG-NITROARGININE METHYL ESTER; NITRIC OXIDE SYNTHASE TYPE III; NITRITES; SIGNAL TRANSDUCTION; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 52249100142     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(08)02001-6     Document Type: Review

References (80)

1. Alessi D.R., James S.R., Downes C.P., Holmes A.B., Gaffney P.R.J., Reese C.B., and Cohen P. Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase B[alpha]. Curr. Biol. 7 (1997) 261-269
2. Ackah E., Yu J., Zoellner S., Iwakiri Y., Skurk C., Shibata R., Ouchi N., Easton R.M., Galasso G., Birnbaum M.J., Walsh K., and Sessa W.C. Akt1/protein kinase Balpha is critical for ischemic and VEGF-mediated angiogenesis. J. Clin. Invest. 115 (2005) 2119-2127
3. Alessi D.R., James S.R., Downes C.P., Holmes A.B., Gaffney P.R.J., Reese C.B., and Cohen P. Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase B[alpha]. Curr. Biol. 7 (1997) 261-269
4. Basso A.D., Solit D.B., Chiosis G., Giri B., Tsichlis P., and Rosen N. Akt Forms an intracellular complex with heat shock protein 90 (Hsp90) and Cdc37 and is destabilized by inhibitors of Hsp90 function. J. Biol. Chem. 277 (2002) 39858-39866
5. Benard V., Bohl B.P., and Bokoch G.M. Characterization of rac and cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases. J. Biol. Chem. 274 (1999) 13198-13204
6. Benard V., and Bokoch G.M. Assay of Cdc42, Rac, and Rho GTPase activation by affinity methods. Methods Enzymol. 345 (2002) 349-359
7. Bernards A. GAPs galore! A survey of putative Ras superfamily GTPase activating proteins in man and Drosophila. Biochim. Biophys. Acta 1603 (2003) 47-82
8. Bernards A., and Settleman J. GAP control: Regulating the regulators of small GTPases. Trends Cell. Biol. 14 (2004) 377-385
9. Bernatchez P.N., Acevedo L., Fernandez-Hernando C., Murata T., Chalouni C., Kim J., Erdjument-Bromage H., Shah V., Gratton J.-P., McNally E.M., Tempst P., and Sessa W.C. Myoferlin regulates vascular endothelial growth factor receptor-2 stability and function. J. Biol. Chem. 282 (2007) 30745-30753
10. Bredt D.S., and Snyder S.H. Nitric oxide mediates glutamate-linked enhancement of cGMP levels in the cerebellum. Proc. Natl. Acad. Sci. USA 86 (1989) 9030-9033
11. Bredt D.S., and Snyder S.H. Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme. Proc. Natl. Acad. Sci. USA 87 (1990) 682-685
12. Breier G. Functions of the VEGF/VEGF receptor system in the vascular system. Semin. Thromb. Hemost. 26 (2000) 553-559
13. +-dependent to the late phosphorylation-dependent activation of the endothelial nitric-oxide synthase in vascular endothelial growth factor-exposed endothelial cells. J. Biol. Chem. 276 (2001) 32663-32669
14. Bryan B.A., and D'Amore P.A. What tangled webs they weave: Rho-GTPase control of angiogenesis. Cell. Mol. Life Sci. 64 (2007) 2053-2065
15. Chamberlain C.E., Kraynov V.S., and Hahn K.M. Imaging spatiotemporal dynamics of Rac activation in vivo with FLAIR. Methods Enzymol. 325 (2000) 389-400
16. Connolly J.O., Simpson N., Hewlett L., and Hall A. Rac regulates endothelial morphogenesis and capillary assembly. Mol. Biol. Cell 13 (2002) 2474-2485
17. Das B., Shu X., Day G.J., Han J., Krishna U.M., Falck J.R., and Broek D. Control of intramolecular interactions between the pleckstrin homology and Dbl homology domains of Vav and Sos1 regulates Rac binding. J. Biol. Chem. 275 (2000) 15074-15081
18. Derakhshan B., Hao G., and Gross S.S. Balancing reactivity against selectivity: The evolution of protein S-nitrosylation as an effector of cell signaling by nitric oxide. Cardiovasc. Res. 75 (2007) 210-219
19. Desire L., Bourdin J., Loiseau N., Peillon H., Picard V., De Oliveira C., Bachelot F., Leblond B., Taverne T., Beausoleil E., Lacombe S., Drouin D., et al. RAC1 inhibition targets amyloid precursor protein processing by gamma-secretase and decreases Abeta production in vitro and in vivo. J. Biol. Chem. 280 (2005) 37516-37525
20. Dimmeler S., Fleming I., Fisslthaler B., Hermann C., Busse R., and Zeiher A.M. Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation. Nature 399 (1999) 601-605
21. Fernandez-Hernando C., Fukata M., Bernatchez P.N., Fukata Y., Lin M.I., Bredt D.S., and Sessa W.C. Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase. J. Cell. Biol. 174 (2006) 369-377
22. Feron O., Belhassen L., Kobzik L., Smith T.W., Kelly R.A., and Michel T. Endothelial nitric oxide synthase targeting to caveolae. Specific interactions with caveolin isoforms in cardiac myocytes and endothelial cells. J. Biol. Chem. 271 (1996) 22810-22814
23. Ferrara N. Role of vascular endothelial growth factor in the regulation of angiogenesis. Kidney Int. 56 (1999) 794-814
24. Ferrara N. The role of VEGF in the regulation of physiological and pathological angiogenesis. Exs. 94 (2005) 209-231
25. +)/calmodulin-dependent endothelial nitric oxide synthase activity. Circ. Res. 88 (2001) E68-E75
26. Fleming I.N., Batty I.H., Prescott A.R., Gray A., Kular G.S., Stewart H., and Downes C.P. Inositol phospholipids regulate the guanine-nucleotide-exchange factor Tiam1 by facilitating its binding to the plasma membrane and regulating GDP/GTP exchange on Rac1. Biochem. J. 382 (2004) 857-865
27. Fleming I.N., Gray A., and Downes C.P. Regulation of the Rac1-specific exchange factor Tiam1 involves both phosphoinositide 3-kinase-dependent and -independent components. Biochem. J. 351 (2000) 173-182
28. Forstermann U., Closs E.I., Pollock J.S., Nakane M., Schwarz P., Gath I., and Kleinert H. Nitric oxide synthase isozymes. Characterization, purification, molecular cloning, and functions. Hypertension 23 (1994) 1121-1131
29. Fryer B.H., and Field J. Rho, Rac, Pak and angiogenesis: Old roles and newly identified responsibilities in endothelial cells. Cancer Lett. 229 (2005) 13-23
30. Fulton D., Gratton J.-P., McCabe T.J., Fontana J., Fujio Y., Walsh K., Franke T.F., Papapetropoulos A., and Sessa W.C. Regulation of endothelium-derived nitric oxide production by the protein kinase Akt. Nature 399 (1999) 597-601
31. Gallis B., Corthals G.L., Goodlett D.R., Ueba H., Kim F., Presnell S.R., Figeys D., Harrison D.G., Berk B.C., Aebersold R., and Corson M.A. Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric oxide production by the phosphatidylinositol 3-kinase inhibitor LY294002. J. Biol. Chem. 274 (1999) 30101-30108
32. Gao Y., Dickerson J.B., Guo F., Zheng J., and Zheng Y. Rational design and characterization of a Rac GTPase-specific small molecule inhibitor. Proc. Natl. Acad. Sci. USA 101 (2004) 7618-7623
33. Garcia-Cardena G., Fan R., Shah V., Sorrentino R., Cirino G., Papapetropoulos A., and Sessa W.C. Dynamic activation of endothelial nitric oxide synthase by Hsp90. Nature 392 (1998) 821-824
34. Garcia-Cardena G., Fan R., Stern D.F., Liu J., and Sessa W.C. Endothelial nitric oxide synthase is regulated by tyrosine phosphorylation and interacts with caveolin-1. J. Biol. Chem. 271 (1996) 27237-27240
35. Garcia-Cardena G., Martasek P., Masters B.S., Skidd P.M., Couet J., Li S., Lisanti M.P., and Sessa W.C. Dissecting the interaction between nitric oxide synthase (NOS) and caveolin. Functional significance of the nos caveolin binding domain in vivo. J. Biol. Chem. 272 (1997) 25437-25440
36. Goligorsky M.S., Abedi H., Noiri E., Takhtajan A., Lense S., Romanov V., and Zachary I. Nitric oxide modulation of focal adhesions in endothelial cells. Am. J. Physiol. 276 (1999) C1271-C1281
37. Guo F., Debidda M., Yang L., Williams D.A., and Zheng Y. Genetic deletion of Rac1 GTPase reveals its critical role in actin stress fiber formation and focal adhesion complex assembly. J. Biol. Chem. 281 (2006) 18652-18659
38. Hall A. Rho GTPases and the control of cell behaviour. Biochem. Soc. Trans. 33 (2005) 891-895
39. He T.-C., Zhou S., da Costa L.T., Yu J., Kinzler K.W., and Vogelstein B. A simplified system for generating recombinant adenoviruses. Proc. Natl. Acad. Sci. USA 95 (1998) 2509-2514
40. Huesken D., Lange J., Mickanin C., Weiler J., Asselbergs F., Warner J., Meloon B., Engel S., Rosenberg A., Cohen D., Labow M., Reinhardt M., et al. Design of a genome-wide siRNA library using an artificial neural network. Nature Biotech. 23 (2005) 995-1001
41. Kim F., Gallis B., and Corson M.A. TNF-alpha inhibits flow and insulin signaling leading to NO production in aortic endothelial cells. Am. J. Physiol. Cell. Physiol. 280 (2001) C1057-C1065
42. Kowanetz M., and Ferrara N. Vascular endothelial growth factor signaling pathways: Therapeutic perspective. Clin Cancer Res. 12 (2006) 5018-5022
43. Kraynov V.S., Chamberlain C., Bokoch G.M., Schwartz M.A., Slabaugh S., and Hahn K.M. Localized Rac activation dynamics visualized in living cells. Science 290 (2000) 333-337
44. Lane P., and Gross S.S. The autoinhibitory control element and calmodulin conspire to provide physiological modulation of endothelial and neuronal nitric oxide synthase activity. Acta Physiol. Scand. 168 (2000) 53-63
45. Lantin-Hermoso R.L., Rosenfeld C.R., Yuhanna I.S., German Z., Chen Z., and Shaul P.W. Estrogen acutely stimulates nitric oxide synthase activity in fetal pulmonary artery endothelium. Am. J. Physiol. 273 (1997) L119-L126
46. Manning B.D., and Cantley L.C. AKT/PKB signaling: Navigating downstream. Cell 129 (2007) 1261-1274
47. McCabe T.J., Fulton D., Roman L.J., and Sessa W.C. Enhanced electron flux and reduced calmodulin dissociation may explain "calcium-independent" eNOS activation by phosphorylation. J. Biol. Chem. 275 (2000) 6123-6128
48. Miao R.Q., Fontana J., Fulton D., Lin M.I., Harrison K.D., and Sessa W.C. Dominant-negative Hsp90 reduces VEGF-stimulated nitric oxide release and migration in endothelial cells. Arterioscler. Thromb. Vasc. Biol. 28 (2008) 105-111
49. Miao R.Q., Gao Y., Harrison K.D., Prendergast J., Acevedo L.M., Yu J., Hu F., Strittmatter S.M., and Sessa W.C. Identification of a receptor necessary for Nogo-B stimulated chemotaxis and morphogenesis of endothelial cells. Proc. Natl. Acad. Sci. USA 103 (2006) 10997-11002
50. +-calmodulin and caveolin. J. Biol. Chem. 272 (1997) 15583-15586
51. Michell B.J., Chen Z., Tiganis T., Stapleton D., Katsis F., Power D.A., Sim A.T., and Kemp B.E. Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase. J. Biol. Chem. 276 (2001) 17625-17628
52. Minami Y., Kawasaki H., Miyata Y., Suzuki K., and Yahara I. Analysis of native forms and isoform compositions of the mouse 90-kDa heat shock protein, HSP90. J. Biol. Chem. 266 (1991) 10099-10103
53. Moffat J., and Sabatini D.M. Building mammalian signalling pathways with RNAi screens. Nature Rev. Cell. Mol. Biol. 7 (2006) 177-187
54. Morales-Ruiz M., Fulton D., Sowa G., Languino L.R., Fujio Y., Walsh K., and Sessa W.C. Vascular endothelial growth factor-stimulated actin reorganization and migration of endothelial cells is regulated via the serine/threonine kinase Akt. Circ. Res. 86 (2000) 892-896
55. Nemoto T., and Sato N. Oligomeric forms of the 90-kDa heat shock protein. Biochem. J. 330 (1998) 989-995
56. Pei Y., and Tuschl T. On the art of identifying effective and specific siRNAs. Nature Methods 3 (2006) 670-676
57. Quilliam L.A., Rebhun J.F., and Castro A.F. A growing family of guanine nucleotide exchange factors is responsible for activation of Ras-family GTPases. Progr. Nucl. Acid Res. Mol. Biol. 71 (2002) 391-444
58. Rickert P., Weiner O.D., Wang F., Bourne H.R., and Servant G. Leukocytes navigate by compass: Roles of PI3Kgamma and its lipid products. Trends Cell. Biol. 10 (2000) 466-473
59. Ridley A.J., Paterson H.F., Johnston C.L., Diekmann D., and Hall A. The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell 70 (1992) 401-410
60. Rousseau S., Houle F., Landry J., and Huot J. p38 MAP kinase activation by vascular endothelial growth factor mediates actin reorganization and cell migration in human endothelial cells. Oncogene 15 (1997) 2169-2177
61. Royal I., Lamarche-Vane N., Lamorte L., Kaibuchi K., and Park M. Activation of cdc42, rac, PAK, and rho-kinase in response to hepatocyte growth factor differentially regulates epithelial cell colony spreading and dissociation. Mol. Biol. Cell 11 (2000) 1709-1725
62. Sander E.E., van Delft S., ten Klooster J.P., Reid T., van der Kammen R.A., Michiels F., and Collard J.G. Matrix-dependent Tiam1/Rac signaling in epithelial cells promotes either cell-cell adhesion or cell migration and is regulated by phosphatidylinositol 3-kinase. J. Cell. Biol. 143 (1998) 1385-1398
63. Sarbassov D.D., Guertin D.A., Ali S.M., and Sabatini D.M. Phosphorylation and regulation of Akt/PKB by the Rictor-mTOR complex. Science 307 (2005) 1098-1101
64. Schleicher M., Shepherd B.R., Suarez Y., Fernandez-Hernando C., Yu J., Pan Y., Acevedo L.M., Shadel G.S., and Sessa W.C. Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence. J. Cell Biol. 180 (2008) 101-112
65. Soga N., Connolly J.O., Chellaiah M., Kawamura J., and Hruska K.A. Rac regulates vascular endothelial growth factor stimulated motility. Cell. Commun. Adhes. 8 (2001) 1-13
66. Soga N., Namba N., McAllister S., Cornelius L., Teitelbaum S.L., Dowdy S.F., Kawamura J., and Hruska K.A. Rho family GTPases regulate VEGF-stimulated endothelial cell motility. Exp. Cell. Res. 269 (2001) 73-87
67. Soldi R., Mitola S., Strasly M., Defilippi P., Tarone G., and Bussolino F. Role of alphavbeta3 integrin in the activation of vascular endothelial growth factor receptor-2. EMBO J. 18 (1999) 882-892
68. Stamler J.S., Lamas S., and Fang F.C. Nitrosylation. The prototypic redox-based signaling mechanism. Cell 106 (2001) 675-683
69. Stamler J.S., Toone E.J., Lipton S.A., and Sucher N.J. (S)NO signals: Translocation, regulation, and a consensus motif. Neuron 18 (1997) 691-696
70. Stephens L., Anderson K., Stokoe D., Erdjument-Bromage H., Painter G.F., Holmes A.B., Gaffney P.R.N.J., Reese C.B., McCormick F., Tempst P., Coadwell J., and Hawkins P.T. Protein kinase B kinases that mediate phosphatidylinositol 3,4,5-trisphosphate-dependent activation of protein kinase B. Science 279 (1998) 710-714
71. Stuehr D.J., and Griffith O.W. Mammalian nitric oxide synthases. Adv. Enzymol. Relat. Areas Mol. Biol. 65 (1992) 287-346
72. Suarez Y., Fernandez-Hernando C., Pober J.S., and Sessa W.C. Dicer dependent microRNAs regulate gene expression and functions in human endothelial cells. Circ. Res. 100 (2007) 1164-1173
73. Venema V.J., Zou R., Ju H., Marrero M.B., and Venema R.C. Caveolin-1 detergent solubility and association with endothelial nitric oxide synthase is modulated by tyrosine phosphorylation. Biochem. Biophys. Res. Commun. 236 (1997) 155-161
74. Waldman S.A., and Murad F. Cyclic GMP synthesis and function. Pharmacol. Rev. 39 (1987) 163-196
75. Waltenberger J., Claesson-Welsh L., Siegbahn A., Shibuya M., and Heldin C.H. Different signal transduction properties of KDR and Flt1, two receptors for vascular endothelial growth factor. J. Biol. Chem. 269 (1994) 26988-26995
76. Welch H.C., Coadwell W.J., Stephens L.R., and Hawkins P.T. Phosphoinositide 3-kinase-dependent activation of Rac. FEBS Lett. 546 (2003) 93-97
77. Whitesell L., Mimnaugh E., Costa B., Myers C., and Neckers L. Inhibition of heat shock protein HSP90-pp60v-src heteroprotein complex formation by benzoquinone ansamycins: Essential role for stress proteins in oncogenic transformation. Proc. Natl. Acad. Sci. USA 91 (1994) 8324-8328
78. Wojciak-Stothard B., Potempa S., Eichholtz T., and Ridley A.J. Rho and Rac but not Cdc42 regulate endothelial cell permeability. J. Cell Sci. 114 (2001) 1343-1355
79. Wojciak-Stothard B., and Ridley A.J. Rho GTPases and the regulation of endothelial permeability. Vascul. Pharmacol. 39 (2002) 187-199
80. Xue Y., Bi F., Zhang X., Pan Y., Liu N., Zheng Y., and Fan D. Inhibition of endothelial cell proliferation by targeting Rac1 GTPase with small interference RNA in tumor cells. Biochem. Biophys. Res. Commun. 320 (2004) 1309-1315