The coenzyme specificity of Candida tenuis xylose reductase (AKR2B5) explored by site-directed mutagenesis and X-ray crystallography

Biochemical Journal

Volumn 385, Issue 1, 2005, Pages 75-83

  Petschacher, Barbara ^a   Leitgeb, Stefan ^a,b   Kavanagh, Kathryn L ^b   Wilson, David K ^b   Nidetzky, Bernd ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Aldo keto reductase; Coenzyme selectivity; NADH; NADPH; Site directed mutagenesis; Xylose fermentation

Indexed keywords

CATALYSIS; CHEMICAL BONDS; CONFORMATIONS; GROUND STATE; MUTAGENESIS; SUBSTRATES;

CO-SUBSTRATES; CONFORMATIONAL SWITCHES; GROUND-STATE BINDING; MUTANTS;

ENZYMES;

FUNGAL ENZYME; NICOTINAMIDE ADENINE DINUCLEOTIDE; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; XYLOSE; XYLOSE REDUCTASE;

ARTICLE; CANDIDA; CANDIDA TENUIS; CRYSTAL STRUCTURE; ENZYME ASSAY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ESCHERICHIA COLI; FERMENTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS; STEADY STATE; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

ADENOSINE; ALDEHYDE REDUCTASE; CANDIDA; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; NAD; NADP; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; RIBOSE; SUBSTRATE SPECIFICITY; THERMODYNAMICS; XYLOSE;

CANDIDA; CANDIDA TENUIS; ESCHERICHIA COLI;

EID: 12844287005     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040363     Document Type: Article

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