Site-directed mutagenesis of the cysteine residues in the Pichia stipitis xylose reductase

FEMS Microbiology Letters

Volumn 147, Issue 2, 1997, Pages 227-232

  Zhang, Yeyan ^a   Lee, Hung ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

aldose reductase; coenzyme binding; cysteine; pentose fermenting; Pichia stipitis; site directed mutagenesis; thiol specific; xylose; yeast

Indexed keywords

OXIDOREDUCTASE; UNCLASSIFIED DRUG; XYLOSE REDUCTASE;

AMINO ACID COMPOSITION; ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; GENE EXPRESSION; MOLECULAR CLONING; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; YEAST;

4-CHLOROMERCURIBENZENESULFONATE; ALDEHYDE REDUCTASE; BINDING SITES; CLONING, MOLECULAR; COENZYMES; CYSTEINE; ENZYME INHIBITORS; MUTAGENESIS, SITE-DIRECTED; NAD; NADP; PICHIA; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SERINE; XYLOSE;

EID: 0031050344     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(96)00531-9     Document Type: Article

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